Searchable abstracts of presentations at key conferences in endocrinology

ea0038p240 | Obesity, diabetes, metabolism and cardiovascular | SFEBES2015

Effects of androgen on the adipokinome and metabolic genes in white and brown adipose tissue

Lerner Avi , Kewada Drashti , Okolo Anthony , Hardy Kate , Franks Stephan

Polycystic ovary syndrome (PCOS) is a common endocrinopathy that is associated with an adverse metabolic profile including insulin resistance and dyslipidaemia. Hyperandrogenism is the hallmark of PCOS and obesity increases androgen synthesis, partly due to accompanying hyperinsulinemia but also as a result of adipokines affecting ovarian steroidogenesis (Comim et al., 2013, PLoS ONE 8(11). Adipokines are factors secreted by adipose tissue and while a clear link betwe...

ea0037gp.09.07 | Nuclear receptors and signalling | ECE2015

Androgen modulates expression of energy-related genes in brown adipocytes

Lerner Avi , Kewada Drashti , Okolo Anthony , Hardy Kate , Franks Stephen

Polycystic ovary syndrome (PCOS) is a common endocrinopathy is associated with an adverse metabolic profile including obesity, insulin resistance, and dyslipidaemia. Hyperandrogenism is the hallmark of PCOS and androgen production is increased in the presence of increased adiposity. While a clear link between obesity and the severity of PCOS exists, the relationship between hyperandrogenism and adipose tissue is less clear. Interestingly, women with PCOS and raised androgen le...

ea0086p123 | Reproductive Endocrinology | SFEBES2022

Androgen receptor splice-variants in granulosa-lutein cells of women with polycystic ovary syndrome (PCOS)

Anujan Priyanka , Lerner Avi , Owens Lisa , Markou Andrea , Hardy Kate , Hanyaloglu Aylin , Bevan Charlotte , Franks Stephen

Defects in any of the four functional domains of the androgen receptor (AR) (resulting from loss-of-function or gain-of-function mutations), may affect androgen action. Specific AR splice variants have been reported in women with PCOS (Wang et al, PNAS 2015 112 4743). In this study we sought to identify AR splice variants in granulosa-lutein (GL) cells of women with and without PCOS and to assess their functional significance. GL cells were ...

ea0038p221 | Obesity, diabetes, metabolism and cardiovascular | SFEBES2015

Altered adipocytes in an ovine model of polycystic ovary syndrome

Siemienowicz Katarzyna , Couckan Flavien , Lerner Avi , Franks Steve , Rae Mick , Duncan Colin

Women with Polycystic Ovary Syndrome (PCOS) are at increased risk of developing insulin resistance, obesity and dyslipidemia, however obesity per se does not explain the higher incidence of insulin resistance in PCOS women when compared to the general population. Altered adipose tissue morphology and function may be a central factor contributing to metabolic disturbances in PCOS. Using a clinically realistic ovine model of PCOS we reported hyperinsulinaemia and early ...

ea0073s13.2 | Symposium 13: TGF-β signalling in ovaries in women with PCOS | ECE2021

LH-receptor activity and interaction with TGF-β family members in women with PCOS

Owens Lisa , Kristensen Stine Gry , Lerner Avi , Hanyaloglu Aylin , Hardy Kate , Andersen Claus Yding , Franks Stephen

The luteinising hormone/chorionic gonadotropin receptor (LHCGR) plays an essential role in ovarian follicular function, mediating the action of LH on theca cell steroidogenesis and, in the preovulatory follicle, on granulosa cell (GC) steroid production and the ovulatory cascade. Following ovulation, LH (and CG) are responsible for maintenance and function of the corpus luteum. Arrested antral follicle development in PCOS is characterised by premature responsiveness of GCs to ...

ea0059oc6.4 | Neuroendocrinology and Reproduction | SFEBES2018

Kisspeptin receptor activity in human granulosa lutein cells

Owens Lisa , Abbara Ali , Lerner Avi , Christopoulous Georgios , Lavery Stuary , Hardy Kate , Dhillo Waljit , Hanyaloglu Aylin , Franks Stephen

Background: Kisspeptin stimulates gonadotropin secretion indirectly by stimulation of hypothalamic GnRH neurons. Kisspeptin and kisspeptin receptor, a G-protein coupled receptor (GPCR), are also expressed in the human ovary, but their direct actions on ovary, if any, are unclear.Objectives: To examine the direct actions of kisspeptin on granulosa lutein cells (GL cells) and the role of kisspeptin in steroidogenesis.Materials and me...

ea0044p211 | Reproduction | SFEBES2016

Insight into the molecular mechanisms underlying alterations in gonadotropin receptor activity in polycystic ovarian syndrome

Owens Lisa , Lerner Avi , Sposini Silvia , Christopoulos George , Khanjani Shiran , Islam Razia , Lavery Stuart , Tsui Vicky , Hardy Kate , Franks Stephen , Hanyaloglu Aylin

Introduction: Polycystic ovary syndrome (PCOS) is a common endocrine disorder, affecting 5–10% of women of reproductive age, and is the major cause of anovulatory infertility. Aberrant secretion and/or action of gonadotropins are implicated but, to date, we have only limited knowledge about the precise mechanisms involved. Recent genome wide association studies have discovered signals at loci close to the genes coding for gonadotropin receptors. The functional significanc...

ea0056oc2.1 | Look who is controlling your gonads! | ECE2018

The in vivo and in vitro effects of kisspeptin on human ovarian function

Owens Lisa , Abbara Ali , Lerner Avi , O'Floinn Shannon , Christopoulos Georgios , Khanjani Shirin , Islam Rumana , Liyanage Maneshka , Hardy Kate , Lavery Stuart , Hanyaloglu Aylin , Dhillo Waljit , Franks Stephen

Background: Infertility is a common problem and the number of couples receiving assisted reproductive treatment (ART) is increasing. Using GnRH agonists (GnRHa) to trigger oocyte maturation has been shown to reduce risk of ovarian hyperstimulation syndrome (OHSS), a common complication of IVF, compared to the more traditional use of human chorionic gonadotropin (hCG). Kisspeptin has recently been shown to be a safe trigger of oocyte maturation in women at high risk of OHSS. Ki...