Searchable abstracts of presentations at key conferences in endocrinology

ea0019p170 | Endocrine tumours and neoplasia | SFEBES2009

The influence of oestrogen on PBF expression, secretion and invasion in MCF7 cells

Watkins RJ , Read ML , Smith VE , Lewy G , Eggo MC , Loubiere LS , Vasilopoulou E , Boelaert K , Franklyn JA , McCabe CJ

Pituitary tumor transforming gene binding factor (PBF) is a relatively uncharacterized gene implicated in endocrine neoplasia. Given the presence of putative oestrogen response elements (ERE) in its promoter, we assessed PBF regulation by oestrogen. PBF mRNA expression was induced maximally at 48 h by 20 nM diethylstilbestrol in ERalpha-positive MCF-7 cells (2.1±0.1-fold, P<0.001, N=6). PBF protein expression levels were also significantly up-regulated b...

ea0019p371 | Thyroid | SFEBES2009

Enlargement of the thyroid gland in a heterozygous PBF knock-in transgenic mouse model

Read M , Fong J , Lewy G , Smith V , Turnell A , Watkins R , Seed R , Stewart S , Warfield A , Leadbeater W , Kim D , Watkinson J , Franklyn J , Boelaert K , McCabe C

PTTG binding factor (PBF) is a poorly characterised 22 kDa protein that is implicated in the aetiology of pituitary and thyroid tumours. Our recent investigations have attempted to discern the function of PBF in vitro. In particular, we reported that PBF can inhibit the activity of the sodium iodide symporter (NIS), which is responsible for iodide uptake into thyroid cells. However, little is known about the precise role of PBF in vivo. We have now generated a mu...

ea0029p1783 | Thyroid cancer | ICEECE2012

Regulation of hPTTG expression and phosphorylation: autocrine interactions with growth factors in thyroid cells

Lewy G. , Ryan G. , Read M. , Fong J. , Seed R. , Sharma N. , Smith V. , Kwan P. , Stewart S. , Warfield A. , Melmed S. , Eggo M. , Franklyn J. , McCabe C. , Boelaert K.

Introduction: The human Pituitary Tumor Transforming Gene (hPTTG) is a phosphorylated proto-oncogene induced in multiple tumour types. hPTTG phosphorylation is mediated by cyclin-dependent kinase 2 (CDC2) and expression is regulated by specificity protein 1 (SP1). In thyroid cancer, hPTTG induces genetic instability and propagates growth through induction of growth factors (GFs).Methods: The interplay between hPTTG phosphorylation, SP1 regulation and GF ...