Searchable abstracts of presentations at key conferences in endocrinology

ea0037ep162 | Reproduction, endocrine disruptors and signalling | ECE2015

Modulation of the circadian clock by glucocorticoid receptor in H295R cell line

Nagy Zsolt , Butz Henriett , Liko Istvan , Racz Karoly , Patocs Attila

Background: Peripheral clocks are set by different nervous, hormonal and metabolic stimuli and regulate the circadian expression of several genes. It has been demonstrated that circadian oscillation of gene expression can be detected in various cell lines in vitro.Aim: To explore whether a peripheral clock could be induced in human adrenocortical cell line H295R and what are the effects of glucocorticoids on this clock system.<p class="abste...

ea0020p566 | Neuroendocrinology, Pituitary and Behaviour | ECE2009

MicroRNA expression in human sporadic pituitary adenomas

Butz Henriett , Liko Istvan , Boyle Belema , Czirjak Sandor , Igaz Peter , Patocs Attila , Racz Karoly

Introduction: MicroRNAs (miRs) are 16–29 nucleotide long, non-coding RNA molecules that post-transcriptionally regulate gene expression via RNA interference. It has been shown that they participate in control of cell proliferation, cell differentiation, signal transduction, cell death and carcinogenesis.Aim: To examine the role of the miRs in sporadic pituitary tumourigenesis.Methods: Twenty-five sporadic pituitary adenoma spe...

ea0037gp.18.06 | Pituitary–Basic and IGF-1 | ECE2015

Comparison of high-throughput platforms in evaluation of whole genome miRNA expression profiles in pituitary tissues

Butz Henriett , Darvasi Otto , Szabo Peter M , Liko Istvan , Pongor Lorinc , Czirjak Sandor , Racz Karoly , Patocs Attila

Introduction: There are three principal high-throughput methods that have been widely used to determine whole genome miRNAs expression profiles: i) microarrays, ii) qPCR based arrays, and iii) next generation sequencing (NGS). Our aim was to compare the results obtained from these platforms in normal and adenomatous pituitary samples and to validate the results by an independent sample set using qPCR.Material and methods: Using four normal pituitary (NP)...

ea0056gp37 | Adrenal cortex | ECE2018

MicroRNA expression profiling in adrenal myelolipoma, adrenocortical cancer and adrenocortical adenoma

Decmann Abel , Perge Pal , Nyiro Gabor , Darvasi Otto , Liko Istvan , Borka Katalin , Micsik Tamas , Patocs Attila , Igaz Peter

Background: Adrenal myelolipoma (AML) is a relatively common and invariably benign tumor composed of adipose tissue and hematopoietic elements. Due to the variable proportion of fat and hematopoietic elements, it is sometimes challenging to differentiate AML from adrenocortical carcinoma (ACC). MicroRNAs have been identified as promising biomarkers in many tumors, including adrenocortical neoplasms, but the microRNA expression of adrenal myelolipoma has not been investigated, ...

ea0056p125 | Endocrine tumours and neoplasia | ECE2018

Heterogeneous genetic background of Hungarian patients with pheochromocytoma/paraganglioma requires gene panel testing

Sarkadi Balazs , Zakarias Sara , Liko Istvan , Grolmusz Vince Kornel , Butz Henriett , Toth Miklos , Szucs Nikolette , Igaz Peter , Patocs Attila

Introduction: Pheochromocytomas and paragangliomas (Pheo/PGL) are rare neuroendocrine tumours arising from the adrenal medulla or the symphathetic paraganglia, respectively. Germline mutations are present in ~40% of the patients. To date, at least 16 genes have been demonstrated to be involved in the genetic background of Pheo/PGL. Prioritization in order of genes tested can be applied, but if the probability of a disease-associated germline mutation exceeds 10% the testing of...

ea0056p779 | Pituitary - Basic | ECE2018

Next generation sequencing for characterization of mitochondrial genome in pituitary adenomas

Nemeth Kinga , Darvasi Otto , Liko Istvan , Szucs Nikolette , Czirjak Sandor , Reiniger Lilla , Szabo Borbala , Igaz Peter , Patocs Attila , Butz Henriett

Introduction: Disrupted mitochondrial functions and genetic variations of mitochondrial DNA (mtDNA) have been observed in different tumors. Regarding pituitary adenomas mtDNA was evaluated only in oncocytic type using PCR based methods and it showed high prevalence of Complex I variants. Next generation sequencing (NGS) allows high throughput sequencing and it is useful for accurate identification of heteroplasmy of mitochondrial genome as well.Aim: We a...

ea0032p533 | Endocrine tumours and neoplasia | ECE2013

Polymorphisms of the glucocorticoid receptor gene, as phenotype modifiers in patients with hormonally inactive adrenal adenomas

Acs Bence , Szappanos Agnes , Feldman-Kovacs Karolina , Liko Istvan , Majnik Judit , Acs Orsolya , Szucs Nikolette , Toth Miklos , Racz Karoly , Patocs Attila

Introduction: Altered sensitivity against glucocorticoids is partly influenced by polymorphisms (SNP) of the glucocorticoid receptor gene (GR). The aim of the present study was to extend our earlier study by inclusion and evaluation the role of the BclI and A3669G polymorphisms of the GR in patients with hormonally inactive adrenal adenomas.Description of methods: This is a retrospective, single-centre genetic association study The study included 99 pati...

ea0020oc1.4 | Endocrine Tumours | ECE2009

ESE Young Investigator Award

Tombol Zsofia , Szabo Peter M , Molnar Viktor , Wiener Zoltan , Horanyi Janos , Riesz Peter , Liko Istvan , Patocs Attila , Gaillard Rolf-Christian , Falus Andras , Racz Karoly , Igaz Peter

Introduction: MicroRNAs (miRNA) are non-coding RNA molecules involved in the posttranscriptional regulation of gene expression. MiRNAs bind mRNA molecules at their 3′ untranslated regions and induce translational repression or target degradation. MiRNAs play important roles in the pathogenesis of several neoplasms. There are no reports, however, on the possible involvement of miRNAs in the pathogenesis of adrenocortical tumors.Objective: To study s...

ea0063p444 | Adrenal and Neuroendocrine Tumours 2 | ECE2019

The Arg244His missense mutation in SDHB-1 leads to altered metabolism in Caenorhabditis elegans: a new disease model

Saskoi Eva , Hujber Zoltan , Liko Istvan , Meszaros Katalin , Sarkadi Balazs , Matyasi Barbara , Kovacs Attila L , Patthy Laszlo , Nyiro Gabor , Svab Gergely , Tretter Laszlo , Mehta Anil , Sebestyen Anna , Patocs Attila , Takacs-Vellai Krisztina

The succinate dehydrogenase (SDH) enzyme complex consisting of four subunits (SDHA, SDHB, SDHC and SDHD) has a dual function in the process of mitochondrial energy generation. It converts succinate to fumarate as part of the TCA cycle and also transfers electrons to ubiquinone as part of the electron transport chain. Mutation in any subunit of the enzyme complex increases the risk for the development of neuroendocrine tumors including paraganglioma (PGL) and pheochromocytoma (...