Searchable abstracts of presentations at key conferences in endocrinology

ea0063p711 | Pituitary and Neuroendocrinology 2 | ECE2019

Whole genome demethylation status of somatic DNA extracted from different pituitary adenoma types

Szabo Borbala , Nemeth Kinga , Meszaros Katalin , Szucs Nikolett , Czirjak Sandor , Reiniger Lilla , Patocs Attila , Butz Henriett

Background: Although the role of hypermethylation of certain tumor suppressor genes are known in pituitary adenomas little information is available regarding whole methylation-demethylation status and especially regarding its correlation with clinical parameters. High-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) is an easy and accurate method to detect the level of methylcytosine (5 mC) and the demethylation intermedier hydroxymethylcytosine (5 hmC) ...

ea0056p779 | Pituitary - Basic | ECE2018

Next generation sequencing for characterization of mitochondrial genome in pituitary adenomas

Nemeth Kinga , Darvasi Otto , Liko Istvan , Szucs Nikolette , Czirjak Sandor , Reiniger Lilla , Szabo Borbala , Igaz Peter , Patocs Attila , Butz Henriett

Introduction: Disrupted mitochondrial functions and genetic variations of mitochondrial DNA (mtDNA) have been observed in different tumors. Regarding pituitary adenomas mtDNA was evaluated only in oncocytic type using PCR based methods and it showed high prevalence of Complex I variants. Next generation sequencing (NGS) allows high throughput sequencing and it is useful for accurate identification of heteroplasmy of mitochondrial genome as well.Aim: We a...

ea0070aep730 | Pituitary and Neuroendocrinology | ECE2020

Global methylation-demethylation status in pituitary neuroendocrine tumors as potential therapeutic target

Szabó Borbála , Németh Kinga , Mészáros Katalin , Szücs Nikolette , Czirják Sándor , Reiniger Lilla , Patócs Attila , Butz Henriett

Background: The altered DNA methylation of certain genes in Pituitary Neuroendocrine Tumors (PitNETs) are well known. However little information is available regarding global methylation changes and the process of demethylation in these tumors. In addition, influencing global methylation-demethylation could be a potential new therapeutic option especially in clinically non-functional PitNETs.Material and Methods: Overall, 44 fresh frozen pituitary adenom...