Searchable abstracts of presentations at key conferences in endocrinology

ea0049gp44 | Bone & Calcium Homeostasis 2 | ECE2017

Investigation of total and free 25OHD vitamin levels in patients with chronic renal failure on different dose of cholecalciferol

Toldy Erzsebet , Kovacs Laszlo , Nemeth Reka , Szakacs Gyulane , Kulcsar Imre , Locsei Zoltan

The total 25-hydroxy-vitamin-D (t-25OHD) level reflects the vitamin-D supply, but it is also influenced by the levels of vitamin-D-binding-proteins (DBP) and albumin. The type of dialysis influences the levels of serum proteins. The ‘free-hormone hypothesis’ states that only the free molecules (f25OHD) can diffuse intracellular. Our aim was to evaluate the total, calculated (c-f25OHD) and direct measured (dm-f25OHD) 25OHD levels in patients with chronic renal disease...

ea0041ep158 | Calcium and Vitamin D metabolism | ECE2016

Investigation of interference with binding proteins in two commonly used 25-hydroxyvitamin D assays

Toldy Erzsebet , Viragh Eva , Horvath Dora Eszter , Kovacs Laszlo , Kovacs Eniko Andrea , Lakatos Peter , Locsei Zoltan

Background: Adequate vitamin D supply is necessary for bone metabolism and also for the general maintenance of health. 25OHD is transported primarily bound to vitamin-D binding protein (DBP), but also to albumin. Lower concentration of plasma proteins may cause difficulties in immunoanalytics. Our aim was to investigate the protein dependence of the two most often used 25OHD immuno- (CLIA) and protein binding- (ECLPBA) assays.Methods: levels were measure...

ea0037ep292 | Calcium and Vitamin D metabolism | ECE2015

Relationship between vitamin D supply and healing in patients with hip fracture

Toldy Erzsebet , Salamon Antal , Hepp Balazs , Matrai Akos , Biro Csaba , Agota Katalin , Fata Emoke , Locsei Zoltan

Background: Vitamin D (VD) deficiency is important risk factor of fractures. Yet, the relation of serum 25-hydroxyvitamin D (25OHD) levels and recovery of fractures is poorly studied. Our aim was to investigate the VD supply in hip fractures also with regard to lifestyle and other clinical (malignancy, kidney and liver disease, osteoporosis) conditions.Method: 25OHD (protein binding assay, Cobas, Roche) and parathyroid hormone (PTHi) (electrochemilumines...

ea0032p113 | Calcium and Vitamin D metabolism | ECE2013

Evaluation of two routinely used 25OHD assays and serum variables in patients on dialyses

Locsei Zoltan , Kovacs Laszlo , Balogh Dora , Szijarto Adrienn , Kalman Bernadette , Kovacs Gabor L , Toldy Erzsebet

The total 25-hydoxy-vitamin-D (t25OHD) level can be assessed by various methods and reflects vitamin D intake. Results are influenced by the serum variables affected by dialyses.Aims: To examine t25OHD and bioavailable vitamin D (b25OHD) by two methods in patients on peritoneal-(PD) and hemodialysis (HD).Methods: We studied 37 HD (64±15 years) and 36 PD (63±18 years) patients. The t25OHD was assessed by chemiluminescence ...

ea0022p61 | Adrenal | ECE2010

Comparison of plasma aldosterone/signal activity and aldosterone/active signal ratios in different clinical conditions

Locsei Zoltan , Toldy Erzsebet , Horvath Dora , Nagy Rita , Racz Karoly , Szabolcs Istvan , Kovacs Gabor L

Because measurement of plasma signal activity (PRA) is time-consuming an automated method directly measuring active signal (REN) may be more suitable for clinical practice. The aim of the study was to analyse the diagnostic utility of REN and aldosterone (ALD)/REN ratio as compared to PRA and ALD/PRA ratio.Materials and methods: PRA, REN (DiaSorin) and ALD (Immunotech) were simultaneously measured in 123 plasma samples (83 females and 40 males; mean age,...

ea0022p246 | Clinical case reports and clinical practice | ECE2010

Serum prolactin levels in rheumatoid arthritis and other autoimmune diseases

Toldy Erzsebet , Opra Barna , Horvath Dora , Locsei Zoltan , Kovacs Gabor L , Racz Karoly

In certain patients with autoimmune diseases (AID) and with rheumatoid arthritis (RA), moderate hyperprolactinemia can be present.Aim: Our aim was to investigate free PRL levels in RA, using two different PRL assays known to react with a different sensitivity to MPRL. The relationship between PRL and the disease-specific autoantibodies (antibody to cyclic citrullinated peptide:cCP-Ab and antinuclear antibody:AN-Ab) were analyzed also.<p class="abstex...

ea0022p833 | Thyroid | ECE2010

Is there a need to constitute laboratory- and/or region-specific reference ranges for the screening of thyroid disorders?

Horvath Dora , Toldy Erzsebet , Locsei Zoltan , Nagy Rita , Kovacs Gabor L , Szabolcs Istvan

Usually, the first laboratory marker for thyroid disorders is TSH. FT4 and FT3 must not be measured unless TSH is out of the reference range. However, the reference range for TSH (and for free thyroid hormones) given by the manufacturers of immunoassays may be not completely valid for screening in iodine-deficient areas and among hospitalized patients either.Aim: Our aim was to constitute specific TSH, FT4, FT3...

ea0014p331 | (1) | ECE2007

From sampling to analytics: experience and diagnostic consequences with some thyroid markers

Toldy Erzsebet , Locsei Zoltan , Szabolcs Istvan , Toldy Erzsebet , Kovacs Gabor L.

In the clinical laboratory practice, endocrine biomolecules are mainly measured by immunoassay. Storage of the samples can not be avoided in many cases. Measurement in the low concentration ranges require exact knowledge on how storage would influence the functional sensitivity of the measurement.Aim: To evaluate the effect of storage of serum samples on their stability and the functional sensitivity of the applied method.Methods: ...

ea0032p114 | Calcium and Vitamin D metabolism | ECE2013

Vitamin D supply in healthy women with different reproductive stages: is there any relationship with DBP levels?

Toldy Erzsebet , Locsei Zoltan , Horvath Dora Eszter , Koppany Csaba , Kovacs Klaudia , Tancsics Emanuela , Kalman Bernadette , Racz Karoly

The total 25-hydoxy-vitamin-D (t-25OHD) level reflects the supply of vitamin D, but is also influenced by the levels of 25OHD binding proteins (DBP). We aimed studying t-25OHD and bioavailable 25OHD (bio-25OHD) levels in healthy females with different reproductive ages.Methods: t-25OHD, bio-intact parathormone (PTHi-1–84) (ECLIA, cobas e411, Roche), DBP (turbidimetry, Dako), calcium and albumin (Alb) levels were measured by routine methods (Modular,...