Searchable abstracts of presentations at key conferences in endocrinology

ea0035p13 | Adrenal cortex | ECE2014

Effects of everolimus, nilotinib, imatinib and ZOL in combination with mitotane in monolayer and spheroids human adrenocortical tumor cell line

Silveira Elaine , Lotfi Claudimara

The primary treatment of adrenocortical carcinoma (ACC) is surgery and the adrenolytic mitotane. In order to test the effect of everolimus, nilotinib, imatinib and zoledronic Acid/ZOL in combination with mitotane were used ACC cell line NCI-H295R, in monolayer (2D) and spheroid (3D) cell cultures. The cytotoxicity promoted by treatments was analyzed by MTS and Cell Death assays. These assays were also performed intending of use them to test drugs in tumor cell cultures from pa...

ea0070aep45 | Adrenal and Cardiovascular Endocrinology | ECE2020

Regression of the adrenal X-zone controlled by JAK pathway in SF1/SOCS3 KO mice

Barabba Auricino Thais , Correa Bento Eduarda , Ferini Pacicco Lotfi Claudimara

Our previous data showed that histological markers of the X-zone in mice adrenal (fetal zone in human adrenal) were still present in adult males and postpartum SF1/SOCS3 KO females. This change led to a distinct distribution of lipid droplets along the adrenal cortex and a blunted ACTH-induced corticosterone secretion in SF1/SOCS3 KO mice. Here we have examined the morphological and functional adrenals of SF1/SOCS3KO male mice adrenals at 3, 8 and 15 weeks through histochemica...

ea0063oc10.4 | Adrenal 2 | ECE2019

Cullin 3 is a partner of armadillo repeat containing 5 (ARMC5), the product of the gene responsible for primary bilateral macronodular adrenal hyperplasia

Cavalcante Isadora , Vaczlavik Anna , Drougat Ludivine , Lotfi Claudimara , Fragoso Maria , Clauser Eric , Rizk-Rabin Marthe , Bertherat Jerome , Ragazzon Bruno

Background: Germline mutations of ARMC5 (Armadillo repeat containing 5 gene) were identified as a frequent cause of primary bilateral macronodular adrenal hyperplasia (PBMAH). ARMC5 is considered as a tumor suppressor gene regulating apoptosis and steroidogenesis by unknown mechanisms. The ARMC5 protein contains a N-terminal domain made of Armadillo (ARM) repeats and a C-terminal ‘Bric-a-Brac, Tramtrack, Broad-complex/Pox virus and Zinc finger (BTB/POZ)&...

ea0049gp6 | Adrenal 1 | ECE2017

Involvement of ARMC5 in proliferation and cell cycle control of human cell cultures from adrenal nodules of primary macronodular adrenocortical hyperplasia (PMAH)

Cavalcante Isadora , Nishi Mirian , Zerbini Maria Claudia , Chambo Jose Luiz , Almeida Madson , Lotfi Claudimara , Fragoso Maria Candida

Background: The mechanisms causing hypercortisolism in primary macronodular adrenocortical hyperplasia (PMAH) are not fully clarified. The participation of ectopic receptors and autocrine/paracrine regulation of intra-adrenal ACTH in hyperplastic tissue have been considered. Additionally, germline ARMC5 mutations have been described as main cause of PMAH. So far, the functional study that analyzed the role of ARMC5 used the adrenocortical carcinoma cell line, H295R. T...

ea0056oc5.5 | Diving deep into adrenal cortex diseases | ECE2018

Cullin 3 is a partner of Armadillo Repeat Containing 5 (ARMC5), the product of the gene responsible for Primary Bilateral Macronodular Adrenal Hyperplasia

Cavalcante Isadora , Clauser Eric , Vaczlavik Anna , Drougat Ludivine , Lotfi Claudimara , Fragoso Maria , Rizk-Rabin Marthe , Bertherat Jerome , Ragazzon Bruno

Background: ARMC5 (armadillo repeat containing 5) has been identified as the gene responsible for PBMAH (Primary Bilateral Macronodular Adrenal Hyperplasia). ARMC5 inactivating mutations are reported in 20 to 25% of PBMAH patients. ARMC5, is considered as a tumor suppressor gene controlling apoptosis and regulating steroidogenesis. The mechanisms of action of ARMC5 are unknown. The structure of the ARMC5 protein contains ARM repeats and a BT...