Searchable abstracts of presentations at key conferences in endocrinology

ea0059p037 | Adrenal and steroids | SFEBES2018

Time-dependent cortisol turnover in tissues using stable isotope tracers and MALDI Mass Spectrometry (MS) sampling

Khan Shazia , Cobice Diego F , Livingstone Dawn EW , Mackay C Logan , Webster Scott P , Walker Brian R , Andrew Ruth

Excess action of glucocorticoid hormones is implicated in metabolic disease and cognitive decline, 11β-hydroxysteroid dehydrogenase 1 (11βHSD1) catalysing generation of active glucocorticoid hormones in tissues. The penetration rates and tissue-specific contribution of 11βHSD1 to glucocorticoid turnover were assessed using tracer kinetics. 9,11,12,12-d4-Cortisol (d4F) was infused (1.75 mg/day, 7 days) into C57Bl6/J male mice and mice lacking 11βHSD1 (n<...