Searchable abstracts of presentations at key conferences in endocrinology

ea0060p13 | (1) | UKINETS2018

Automated finger prick genomic diagnosis of neuroendocrine tumors

Modlin Irvin , Kidd Mark , Drozdov Ignat , Bodei Lisa , Malczewska Anna , Matar Somer , Chung Kyung-Min

Background: The diagnosis of neuroendocrine tumor disease has been tardy due to a lack of sensitive, noninvasive technology to facilitate identification. In the 1960’s, diagnosis required tumor tissue attained by surgery or biopsy. Identification was by histopathology and immunohistochemistry (1975). Acquisition of information was limited by the morbidity of invasive strategies, inability to reassess and interpretation was user-dependent with low kappa values. The introdu...

ea0060p16 | (1) | UKINETS2018

Validation of the NETest liquid biopsy as a diagnostic for gastroenteropancreatic neuroendocrine tumors (GEP-NETs)

Malczewska Anna , Kos-Kudla Beata , Witkowska Magdalena , Walter Agata , Makulik Karolina , Bocian Agnes

Background: The NETest, a neuroendocrine multigene transcript ‘liquid biopsy’, is a novel biomarker for GEP-NETs. Current biomarkers are monoanalyte amines or peptides whereas the NETest is a multianalyte signature providing molecular biological information pertinent to clinical disease.Aim: Independently validate the utility of the NETest for the diagnosis of GEP-NETs and identification of disease progression.Material an...

ea0060p15 | (1) | UKINETS2018

NETest liquid biopsy is diagnostic of lung neuroendocrine tumors and identifies progressive disease

Malczewska Anna , Kos-Kudla Beata , Filosso Pier-Luigi , Aslanian Harry , Lewczuk Anna , Cwikla Jaroslaw , Rofinella Mateo , Bodei Lisa

Background: There are no effective blood biomarkers for bronchopulmonary carcinoids (BPC). We examined the utility of a neuroendocrine multigene transcript ‘liquid biopsy’ (NETest) in bronchopulmonary carcinoids (BPC) for diagnosis and monitoring of disease status.Aim: To independently validate the utility of the NETest in diagnosis and management of BPC in a prospective multicenter, multinational blinded study.Material a...

ea0052p26 | (1) | UKINETS2017

Multi-parametric assessment improves prognostication of small bowel neuroendocrine neoplasms: external validation of the NET nomogram

Clift Ashley , Faiz Omar , Martin John , Wasan Harpreet , Liedke Marc-Olaf , Schloericke Erik , Malczewska Anna , Rindi Guido , Kidd Mark , Modlin Irvin , Frilling Andrea

Background: Small-bowel neuroendocrine tumours (SB-NET) commonly metastasise despite most being low grade. The NET nomogram developed, estimates 5- and 10-year survival in SB-NET by allocating scores for 15 clinicopathological parameters. We comparatively evaluated the prognostic power of this nomogram, the WHO/ENETS grading and AJCC/UICC staging systems.Methods: Patients with histologically-confirmed SB-NET were identified from databases at two tertiary...

ea0070aep567 | Pituitary and Neuroendocrinology | ECE2020

Multigene liquid biopsy (NETest) is diagnostic of pancreatic and small bowel neuroendocrine tumours and correlates with imaging

Malczewska Anna , Witkowska Magdalena , Makulik Karolina , Bocian Agnes , Walter Agata , Wojcik-Giertuga Monika , Pilch-Kowalczyk Joanna , Zajecki Wojciech , Bodei Lisa , Oberg Kjell , Kos-Kudla Beata

Introduction: There is a substantial unmet clinical need for an accurate and effective blood biomarker for gastroenteropancreatic neuroendocrine tumours (GEP-NETs). Current monoanalyte biomarkers are ineffective. The NETest, a novel multianalyte signature, provides molecular information relevant to disease biology. We therefore evaluated, under real-world conditions, the clinical utility of the NETest as a liquid biopsy in GEP-NETs.Aim(s): Independently ...

ea0052p02 | (1) | UKINETS2017

Validation of a blood biomarker test for the diagnosis and management of bronchopulmonary neuroendocrine tumors

Modlin Irvin , Kidd Mark , Lewczuk Anna , Chung Kjung-Min , Kolasinska-Cwikla Agnieska , Cwikla Jaroslaw , Lowczak Anna , Doboszynska Anna , Tesselaar Margot , Buikhuisen Wieneke , Malczewska Anna , Kos-Kudla Beata , Roffinella Matteo , Filosso Pier Luigi , Korse Tiny , Papotti Mauro , Bodei Lisa , Drozdov Ignat

Background: There is currently no effective blood biomarker for lung neuroendocrine neoplasia diagnosis and management. We describe the clinical utility of a 51 neuroendocrine-specific gene expression set in blood to diagnose bronchopulmonary neuroendocrine tumors (BPNETs) and define their clinical status.Methods: The discovery set included BPNETs (n=154) and controls (n=90), randomly assigned (1:1) to a test and validation set. Specifi...

ea0052p17 | (1) | UKINETS2017

Surgical therapy for appendiceal neuroendocrine tumours: is appendicectomy adequate?

Clift Ashley , Pawa Nikhil , Drymousis Panagiotis , Cichocki Andrzej , Flora Rashpal , Goldin Rob , Patsouras Dimitrios , Baird Alan , Malczewska Anna , Kinross James , Faiz Omar , Antoniou Anthony , Wasan Harpreet , Kaltsas Gregory , Darzi Ara , Cwikla Jaroslaw , Frilling Andrea

Background: Neuroendocrine tumours of the appendix (ANET) are relatively indolent tumours typically identified incidentally at surgery for suspected appendicitis. The role of right hemicolectomy (RH) for tumours with ‘high risk’ features is debated. We compared the management of ANET at three centres against ENETS criteria for therapy selection.Methods: Retrospective review of all patients diagnosed with ANET at three tertiary centres. Patients...

ea0046p1 | (1) | UKINETS2016

MicroRNAs associated with small bowel neuroendocrine tumours and their metastases

Miller Helen C , Frampton Adam E , Malczewska Anna , Ottaviani Silvia , Stronach Euan A , Flora Rashpal , Kaemmerer Daniel , Schwach Gert , Pfragner Roswitha , Faiz Omar , Kos-Kudla Beata , Hanna George B , Stebbing Justin , Castellano Leandro , Frilling Andrea

Introduction: Novel molecular analytes are needed in small bowel neuroendocrine tumours (SBNETs) to better determine disease aggressiveness and predict treatment response.Aim of the study: To profile the global miRNome of SBNETs, and identify microRNAs (miRNAs) involved in tumour progression for use as potential biomarkers.Material and methods: Two independent miRNA profiling experiments were performed (n=90), including pr...

ea0072p1 | (1) | UKINETS2020

Post-operative NETest scores detect residual NET disease and accurately predicts tumor recurrence in R0

Modlin Irvin , Kidd Mark , Oberg Kjell , Falconi Massimo , Luigi Filosso5 Pier , Frilling Andrea , Malczewska Anna , Salem Ronald , Toumpanakis Christos , Laskaratos Faidon-Marios , Partelli Stefano , Roffinella Matteo , Arx Claudia von , Kos-Kudla Beata , Bodei Lisa , Drozdov Ignat , Kitz Alexandra

Introduction: Surgery is the only cure for neuroendocrine tumor (NET) disease. R0 resection is critical for successful tumor resection. Early detection of residual disease is key for optimal management. Both imaging and current biomarkers have intrinsic limitations and are largely ineffective up to 3 months post-surgery. NETest, a multigene blood biomarker test, identifies NETs with >90% accuracy. We hypothesized that surgery would decrease NETest levels and that elevated scor...