Searchable abstracts of presentations at key conferences in endocrinology

ea0065p169 | Metabolism and Obesity | SFEBES2019

Intravascular subcutaneous adipose tissue blood flow measured with Doppler ultrasound for experimental medicine studies

Lempesis Ioannis , Goossens Gijs , Manolopoulos Konstantinos

Background: Adipose tissue blood flow (ATBF) is important for delivering nutrients and oxygen to adipose tissue (AT), and distributing adipokines and metabolites into the circulation. ATBF has been measured with the 133Xenon wash-out technique (gold standard), microdialysis, laser Doppler Fluximetry, and contrast-enhanced ultrasound. However, due to decline in world-wide 133Xenon production and the invasive nature of other techniques, an alternative metho...

ea0065p199 | Metabolism and Obesity | SFEBES2019

Systemic and femoral adipose tissue-specific nontargeted plasma metabolome during acute hypercortisolaemia

Prete Alessandro , Dunn Warwick B , Manolopoulos Konstantinos

Background: Glucocorticoids have pleiotropic metabolic functions and acute glucocorticoid excess causes dramatic disruption of human metabolism. Whether glucocorticoids exert adipose tissue depot-specific effects on the nontargeted metabolome in unknown.Aim: Assess the nontargeted metabolome in the systemic circulation and in femoral adipose tissue-specific blood samples in response to physiological hyperinsulinaemia and acute hypercortisolaemia.<p c...

ea0065p382 | Reproductive Endocrinology and Biology | SFEBES2019

An evaluation of the current clinical care pathway of patients referred to a large UK Tertiary Centre with suspected PCOS

Bajaj Mitali , Elhassan Yasir , Gleeson Helena , Manolopoulos Konstantinos

Background: PCOS is a common female endocrine disorder, exacerbated by obesity. International guidelines therefore suggest weight loss as first line management. This service evaluation assessed the care pathway of patients referred to Queen Elizabeth Hospital Birmingham (QEHB) with suspected PCOS. We aimed to assess the referral wait time, reason for referral, treatment offered, and weight management in clinic.Methods: We undertook a cross-sectional stud...

ea0038p191 | Obesity, diabetes, metabolism and cardiovascular | SFEBES2015

Hypercortisolaemia increases femoral adipose tissue blood flow but not net fatty acid release in vivo

Manolopoulos Konstantinos , Bujalska Iwona , Luzio Steve , Arlt Wiebke , Tomlinson Jeremy

Hypercortisolaemia (Cushing’s Syndrome) is characterised by abdominal fat accumulation and gluteofemoral fat loss. The mechanisms underpinning this fat mass redistribution are unknown. Adipose tissue blood flow (ATBF) and net fatty acid release rate (lipolysis) are important determinants of depot-specific fat mass. We hypothesized that hypercortisolaemia may result in increased femoral ATBF and lipolysis promoting gluteofemoral fat mass loss. We recruited nine healthy mal...

ea0034p98 | Clinical practice/governance and case reports | SFEBES2014

Outcomes following pituitary surgery: a single centre audit

Manolopoulos Konstantinos , Bates Andrew S , Shad Amjad , Rahim Assad

Introduction: Pituitary surgery is associated with post-operative complications including hypopituitarism and visual field defects. In order to ensure that our surgical outcomes were comparable with other centres, we audited the outcome of a cohort of patients referred for surgery at a single centre.Methods: A retrospective audit of 27 consecutive patients diagnosed with pituitary adenoma at Heartlands Hospital Birmingham, who underwent trans-sphenoidal ...

ea0081p449 | Reproductive and Developmental Endocrinology | ECE2022

Global impact of PCOS awareness month: challenges and opportunities

Malhotra Kashish , Synn Cuen Pan Carina , Davitadze Meri , Manolopoulos Konstantinos , O'Reilly Michael , Arlt Wiebke , Kempegowda Punith

Background: September is celebrated as PCOS awareness month worldwide and is aimed at improving the lives of those with PCOS, promoting research, and strengthening advocacy efforts. While significant financial and human resources are invested in promotions during this month, to the best of our knowledge, there is no evidence to show the impact of this initiative. In our study, we evaluated the global digital impact of PCOS Awareness Month, tracking commonly used hashtags as su...

ea0038p219 | Obesity, diabetes, metabolism and cardiovascular | SFEBES2015

Non-selective beta-adrenergic agonist infusion acutely stimulates the temperature of brown adipose tissue in adult males

Scotney Hannah , Symonds Michael , Law James , Budge Helen , Sharkey Don , Manolopoulos Konstantinos

Brown adipose tissue (BAT) is present in small quantities in human adults (~100 g) and can have a significant influence on metabolism. This effect is mediated by rapid activation of the BAT specific uncoupling protein 1 (UCP1), following stimulation of β-adrenergic receptor (AR) by the sympathetic nervous system. AR agonists stimulate rodent BAT, but have so far provided inconsistent findings in humans when administered orally (Vosselman et al. 2012). Rapid activ...

ea0081ep355 | Diabetes, Obesity, Metabolism and Nutrition | ECE2022

Distinct inflammatory signatures of upper- and lower-body adipose tissue in postmenopausal women with normal weight and obesity

Lempesis Ioannis , Hoebers Nicole , Essers Yvonne , Jocken Johan WE , Blaak Ellen E , Manolopoulos Konstantinos N , Goossens Gijs H

Background: Abdominal obesity is associated with insulin resistance and increased cardiometabolic disease risk, whereas lower-body fat accumulation seems protective against metabolic derangements. Differences in upper-body and lower-body adipose tissue (AT) function seem to underlie these opposing associations. Here, we investigated the inflammatory signature of upper- and lower-body AT in women with normal weight and obesity, as well as in human primary abdominal (ABD) and fe...

ea0049oc3.3 | Receptors &amp; Signalling | ECE2017

AKR1C3-mediated adipose androgen generation drives lipotoxicity in polycystic ovary syndrome

O'Reilly Michael , Kempegowda Punith , Walsh Mark , Taylor Angela , Manolopoulos Konstantinos , Allwood William , Semple Robert , Hebenstreit Daniel , Dunn Warwick , Tomlinson Jeremy , Arlt Wiebke

Polycystic ovary syndrome (PCOS) is prevalent metabolic disorder in women, associated with androgen excess and insulin resistance. These two major features are closely correlated, but the direction of causality remains unclear. Aldoketoreductase type 1C3 (AKR1C3) converts the androgen precursor androstenendione to testosterone (T), and is highly expressed in subcutaneous (SC) adipose tissue. We hypothesised that adipose tissue represents an important site linking androgen acti...

ea0059cc6 | Featured Clinical Cases | SFEBES2018

What lies beneath: cutaneous Kaposi’s sarcoma as a first manifestation of ectopic ACTH-dependent Cushing’s syndrome

Tresoldi Alberto S , Elhassan Yasir S , Asia Miriam , Elshafie Mona , Lane Peter , Manolopoulos Konstantinos N , Velangi Shireen S , Watkins Steven , Arlt Wiebke , O'Reilly Michael W

Introduction: Immune dysregulation is a feature of Cushing’s syndrome (CS). We report a case of CS that presented with rapidly developing cutaneous Kaposi’s sarcoma (KS).Case description: A previously well 59-year-old heterosexual man presented with a two-month history of proximal muscle weakness, recurrent mouth ulcers, and purplish skin lesions. He had a background history of hypertension. Skin biopsies were compatible with KS. History of pas...