Searchable abstracts of presentations at key conferences in endocrinology

ea0011p762 | Steroids | ECE2006

Differential regulation of Cyp4A isoforms in mouse kidney during the development of mineralocorticoid, salt-sensetive induced hypertension

Marshall E , Roy D , Mullins J , Kenyon C , Brown R

Arachidonic acid (AA) metabolites derived from cytochrome P450 enzymes regulate vascular tone and renal tubular function. The cytochrome P450 4A (Cyp4A) enzymes are responsible for the synthesis of 20- hydroxyeicosatetraenoic (20-HETE), the most abundantly produced AA Cyp P450 metabolite in the kidney. Cyp4a expression and the production of 20-HETE, have been implicated in the development of hypertension. Here we have investigated the expression of various renal isoforms of Cy...

ea0009p138 | Steroids | BES2005

WNK kinases; a novel pathway regulating electrolyte balance and blood pressure, which is mutated in Pseudohypoaldosteronism type II

O'Reilly M , Marshall E , Mittal M , Kenyon C , Brown R

WNK1 and WNK4 are serine/threonine kinases, With-No-K (lysine) residue at a key catalytic position within the active site. Mutations in either cause Pseudohypoaldosteronism type II (Gordon syndrome), an autosomal dominant, hypertensive, hyperkalaemic disorder, especially responsive to thiazide diuretics (first line treatment for essential hypertension). This novel WNK pathway is implicated in normal regulation of blood pressure (BP) and distal nephron Na+/K+</s...

ea0015p369 | Thyroid | SFEBES2008

Raised serum FT4 and inappropriately detectable serum TSH: evaluation of recommendations from the UK TFT guidelines

Han T S , Woolman E , Marshall K , Thomas M , Vanderpump M

Objectives: The UK TFT Guidelines (2006) recommend screening for heterophilic antibodies (HA), thyroid hormone resistance (THR) and TSHoma in samples with raised FT4 and detectable or raised TSH levels. The aim was to determine the frequency and aetiology of such ¬Ďabnormal¬í thyroid function tests (TFTs) and to evaluate the use of further analyses for this common biochemical abnormality.Design: Six-month retrospective study (1/08/06&#...

ea0011p397 | Diabetes, metabolism and cardiovascular | ECE2006

Dietary potassium driven responses in the renal WNK kinase pathway in vivo

O’Reilly M , Marshall E , MacGillvray T , Mittal M , Kenyon CJ , Brown RW

WNK1 (With-No-K, lysine) and WNK4 are serine/threonine kinases, mutated in Gordon syndrome (Pseudohypoaldosteronism Type II), a dominant, hypertensive, hyperkalaemic disorder; implicating this novel WNK pathway in normal regulation of blood pressure (BP) and electrolyte balance. Previous Xenopus oocyte work implicates WNK4 in regulation of K+ secretion via ROMK (renal outer medullary K+ channel) and Cl- transport pathways both paracellularly via tight junctions (claudin...

ea0009p139 | Steroids | BES2005

Profiling aldosterone and salt responsive pathways in an inducible hypertensive mouse model

Marshall E , Forster T , Dickenson P , Roy D , Mullins J , Kenyon C , Brown R

The kidney plays a dominant role in long-term blood pressure (BP) control and electrolyte homeostasis but aldosterone (aldo) responsive molecular pathways in the kidney involved in regulating BP remain poorly understood despite identification of several participant genes. Many human single gene disorders affecting BP have been faithfully reproduced in mice by targeting the corresponding genes. Thus mouse is an excellent model to study molecular pathways underlying hypertension...

ea0007p225 | Steroids | BES2004

Gene expression responses induced in kidney by aldosterone excess and salt

Marshall E , Dickenson P , Forster T , Roy D , Kenyon C , Brown R

Renal sodium handling and responses to aldosterone (aldo) are conserved across species and intrinsic to the dominant role of the kidney in long-term blood pressure (BP) control. Molecular pathways involved in hypertensive responses to aldo and escape from these remain largely unclear despite identification of several participant genes. We have characterized renal gene expression changes in mice, receiving aldo and/or dietary salt excess, using microarray analysis and concurren...

ea0005p231 | Steroids | BES2003

Development of a mouse hypertension model induced by aldosterone excess

Marshall E , Speirs H , Coan S , Mullins J , Kenyon C , Brown R

The pathway from aldosterone to upregulation of renal sodium re-absorption via the amiloride sensitive sodium channel (ENaC) is strongly implicated in blood pressure control and causation of hypertension (HT) in humans. In order to study these processes further an animal model is required to correlate physiological changes as hypertension develops with the molecular pathways underpinning these in kidney. Transgenic studies indicate mouse is an excellent species for modeling th...