Searchable abstracts of presentations at key conferences in endocrinology

ea0005p207 | Reproduction | BES2003

Polycystic ovary syndrome (PCOS) has a significant negative impact on health-related quality of life (HRQoL)

Coffey S , Bano G , Mason H

PCOS is a chronic medical condition consisting of PCO on ultrasound in conjunction with menstrual cycle disturbance, insulin resistance, obesity and/or hyperandrogenism manifest as acne, hirsutism and androgenic alopecia. These symptoms can be expected to have a significant impact on quality of life. The aim of this study was to compare the health-related quality of life (HRQoL) of women with PCOS with that of women in the general population.HRQoL is generally measured by ...

ea0011p687 | Reproduction | ECE2006

Anti-Mullerian hormone (AMH) production by and amh type-II receptor (AMHRII) in normal human ovaries

Hanna L , Pellatt L , Rice S , Whitehead S , Mason H

AMH, the fetal testicular sexual differentiative factor, is now implicated in adult ovarian function. Antral follicle immunohistochemistry demonstrated AMH protein and message, the staining peaking around 4 mm. Interestingly, AMH-knockout mice have increased FSH sensitivity. Our aim was to measure AMH in follicular fluid and cell-conditioned medium and AMHRII in normal ovaries from women undergoing TAH/BSO.Follicles were dissected intact, follicular flui...

ea0011p712 | Reproduction | ECE2006

Stage-specific mRNA expression of androgen receptor correlated with FSH receptor in individual pre-antral follicles isolated from human ovary

Rice S , Ojha K , Whitehead S , Mason H

Recent evidence indicates that the increase in follicle numbers seen in PCOS occurs early in folliculogenesis, and that androgens are implicated in this. In primates and sheep, androgen excess in-utero results in ovarian changes similar to those in PCOS. We have recently shown using a novel in ovo model, that testosterone added to implanted human tissue increased primary follicles compared to untreated tissue (Qureshi et al. 2005). This is similar to the p...

ea0007p181 | Reproduction | BES2004

The CAM technique and follicle development

Qureshi A , Bano G , Whitehead S , Nussey S , Mason H

BackgroundCurrent techniques to study the early stages of follicular development (i)in vitro(/i) are handicapped by the spontaneous wholesale transition of primordial to primary follicles and by primary follicle arrest. We have circumvented this problem by culturing fragments of ovarian cortical tissue on the chorioallantoic membrane (CAM) of chick embryos and shown it to be successful for the (i)in ovo(/i) culture of cortical slices from a variety of sp...

ea0006p57 | Reproduction | SFE2003

Ovarian follicle culture: an old technique revisited

Qureshi A , Bano G , Whitehead S , Nussey S , Mason H

Current techniques to study (i)in vitro(/i) the early stages of follicular development are handicapped by the spontaneous wholesale transition of primordial to primary follicles. Implantation of ovarian tissue into SCID mice is possible but expensive. We have, therefore, adapted a model first described in 1958. Fragments of ovarian cortex are implanted on the chorioallantoic membrane (CAM) of 5-6 day old fertilised chick eggs. The membrane is rich in blood vessels and results ...

ea0005p209 | Reproduction | BES2003

Immunopurification of gonadotrophin surge-attenuating factor (GnSAF) bioactivity

Sorsa-Leslie T , Harris W , Mason H , Fowler P

Objective We aimed to immunopurify GnSAF, using antibodies with demonstrated GnSAF bioactivity-blocking/binding activity, in order to confirm 2 new candidate amino acid sequences for human ovarian GnSAF (Fowler et al. Mol Human Reprod 2002;8:823-832).Methods Rat antiserum, raised against a 60-70 kDa band of partially purified GnSAF, was immobilised on anti-rat IgG Dynabeads. In addition, a human antibody, derived from a phage display antibody library a...

ea0003p231 | Reproduction | BES2002

Engineering of single chain antibodies selected from a phage display library into human IgGs that recognise gonadotrophin surge-attenuating factor (GnSAF) bioactivity

Sorsa-Leslie T , Harris W , Mason H , Fowler P

Introduction. We have previously used a synthetic phage display library to derive single chain (ScAbs) antibodies which bind GnSAF bioactivity in-vitro. However, recovery of GnSAF using the ScAbs, which tended to aggregate and become unstable, was limited. We have therefore engineered these ScAbs into full-length human IgG forms.Methods. CDR regions from soluble expression plasmids for two different GnSAF-recognising ScAbs were amplified by PCR. Two uni...

ea0003p232 | Reproduction | BES2002

1,25-dihydroxyvitamin D3 receptors (VDR) are present on human granulosa cells and cells of the corpus luteum

Brain H , Bano G , Murray J , Nussey S , Mason H

There is evidence from animal studies that 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3) is essential for normal reproductive function. Vitamin D deficient rats have reduced fertility, and VDR null mutant mice demonstrate impaired folliculogenesis. We have recently demonstrated that 1,25(OH)2D3 suppressed the production of oestradiol (E2) independently of progesterone (P) in luteinised, but not in non-luteinised human granulosa cells, suggesting that 1,25(OH)<s...

ea0002oc22 | Growth Regulation | SFE2001


Wright R , Perks C , Holly J , Mason H

IGFs act as amplifiers of gonadotrophin (Gn) action in the ovary, but their activity is modulated by IGFBPs. In addition, we have shown IGF-independent inhibitory effects of IGFBP-4 on ovarian steroidogenesis. The aim of this study was to investigate the possible mechanism of action of IGFBP-4 in ovarian cells.Granulosa cells (GC) and theca were harvested from intact follicles (2.5-14mm) dissected from 8 pairs of ovaries collected with ethics committee a...

ea0002oc26 | Reproduction | SFE2001


Perks C , Wright R , Mason H , Holly J

The role of prolactin in the regulation of ovarian folliculogenesis and in particular it's relationship to follicular atresia is as yet unclear; although a decline in prolactin concentrations in bovine follicular fluid appears to be related to morphological signs of atresia. In addition to the pituitary and endometrium sources of prolactin, there is also a local ovarian source of prolactin. Hence a potential autocrine mechanism of prolactin action may exist in the ovary. We in...