Endocrine Abstracts

GPR120 is a rhodopsin-like GPCR that has a high affinity for long-chain saturated fatty acids 14–18 carbons and unsaturated fatty acids 16–22 carbons. The beneficial effects of GPR120 on β-cell function and glucose homeostasis have recently been reported however its role in glucagon secretion is unknown. GPR120 and glucagon expression were examined by double immunohistochemical studies using a glucagon secreting cell line (α-TC1.9) and pancreatic tissue fro...

Background: G-Protein-Coupled Receptor GPR55, a member of the endocannabinoid system, has gained significant interest as a treatment for diabetes. This study aimed to investigate GPR55 as a drug target using a novel GPR55-agonist, ML-184 (high selectivity EC50=0.26µM), and the downstream induction of signalling pathways.Methods: High-fat-fed (HFF) C57BL/6 mice were treated with a daily oral dose of ML-184 (0.1μmol/kg) alone or in combination wi...

Background: G-Protein Coupled Receptors-120 (GPR120) and GPR40 are of interest as targets for treating diabetes and obesity due to their involvement in incretin secretion. This study investigated the mechanistic function of dual agonism on regulating the regeneration of beta cell mass, proliferation, and the insulin secretory response, using CRISPR/Cas9 gene knockout cells and intracellular-signalling analysis.Methods: Clonal pancreatic BRIN-BD11 cells a...