Searchable abstracts of presentations at key conferences in endocrinology

ea0025p244 | Pituitary | SFEBES2011

BAC recombineering to understand the role of the alternative promoter in the regulation of prolactin expression

Awais Raheela , McNamara Anne , Harper Claire , Adamson Anthony , Spiller Dave , Semprini Sabrina , Mullins John , Davis Julian , White Michael R H

Alternative promoters control many genes including prolactin (PRL), which in man is expressed at extra-pituitary sites controlled by an alternative promoter located 5.8 Kbp upstream of the pituitary transcription start site. Previous studies using short promoter fragments may be misleading, as the human hPRL genomic locus has many conserved far-upstream regions. To study the function of the alternative promoter, we engineered a bacterial artificial chromosome (BAC) expressing ...

ea0021p298 | Pituitary | SFEBES2009

Onset of prolactin gene transcription in nascent pituitary lactotroph cells

Featherstone Karen , Harper Claire , McNamara Anne , Semprini Sabrina , Spiller David , McNeilly Alan , Mullins John , White Michael , Davis Julian

Pituitary development, in particular the differentiation of anterior pituitary endocrine cells, remains to be fully understood, and may have implications for adult pituitary plasticity and hyperplasia. We have used prolactin-reporter transgenic rats to characterise prolactin transcription during the appearance of newly formed lactotroph cells during fetal development.Transgenic rats with the firefly luciferase reporter gene inserted into exon 1b of a 160...

ea0021p299 | Pituitary | SFEBES2009

Immuno-modulatory role of prolactin in immune tissues driven by the alternative promoter

McNamara Anne , Awais Raheela , Semprini Sabrina , Harper Claire , Featherstone Karen , Spiller Dave , Davis Julian , Mullins John , White Mike

Prolactin (PRL) is a hormone mainly produced by the lactotroph cells of the anterior pituitary gland. Besides its pivotal role in reproduction, PRL has also been found to have immuno-modulatory properties. The pituitary is the main source of circulating PRL, however it is also expressed in humans at extra-pituitary sites1. In extra-pituitary tissues, PRL mRNA contains an extra exon (exon 1a), and expression is regulated by an alternative promoter upstream of the pit...

ea0034oc5.5 | Pituitary | SFEBES2014

Spatio-temporal analysis of prolactin gene transcription dynamics reveals short range co-ordination of lactotroph transcription activity in pituitary tissue

Featherstone Karen , Hey Kirsty , Momiji Hiroshi , McNamara Anne , Patist Amanda , Spiller David , Christian Helen , McNeilly Alan , Mullins John , Finkenstadt Barbel , Rand David , White Michael , Davis Julian

Mammalian genes display pulsatile transcription dynamics with bursts of expression occurring with variable duration and frequency. Using the human prolactin gene as a model of tissue specific gene regulation, we have characterised the transcription dynamics of this gene in cell lines, primary cells and pituitary tissue slices. Our data indicate that the tissue environment may have an important influence on cellular transcription activity. Cultures of cells from enzymatically d...