Searchable abstracts of presentations at key conferences in endocrinology

ea0044se1.2 | Senior Endocrinologists' Session | SFEBES2016

Conservation endocrinology: What, are there more than two species to consider?

McNeilly Alan

Conservation is a tricky subject to discuss when people actually are asked to consider the present situation, and the virtual world that many live in today. Many of the losses of species are due to human intervention, through killing for pleasure, food, or profit (greed), or through habitat destruction. The species are innocent. Zoos provide a sanctuary for maintaining some numbers of species on a limited scale, but can only ever be a stop-gap to maintain the species, although...

ea0015s1 | Society for Endocrinology Dale Medal Lecture | SFEBES2008

Hormones and ovaries: a lifelong journey

McNeilly Alan

Birth of a single baby gives that infant the best possible start to life. In mono-ovular species like man there is a tight control over the hypothalamic–pituitary–ovarian axis to try to ensure that there is also sufficient time between births to allow the infant to develop to its full potential. Breastfeeding suppresses fertility by modulating pulsatile LH but not FSH secretion delaying the final stages of ovarian follicle maturation and the return of fertility in br...

ea0015s1biog | Society for Endocrinology Dale Medal Lecture | SFEBES2008

Society for Endocrinology Dale Medal Lecture

McNeilly Alan

Alan McNeilly, MRC Human Reproduction Sciences Unit, Edinburgh, UK AbstractAfter graduating from Nottingham (BSc) and Reading (PhD) Universities with degrees in animal physiology, Dr McNeilly joined Tim Chard at Barts for 4 years developing gonadotrophin and prolactin assays for clinical application. During those 4 years he gained experience in clinical endocrinology. Collaborations with Roger Short and David Baird led...

ea0028p316 | Steroids | SFEBES2012

Impact of prenatal androgens on glucocorticoid metabolism in an ovine model of PCOS.

McIlvride Saraid , Ellison Alicia , Hogg Kirsten , McNeilly Alan , Duncan Colin , Michael Anthony

Within target cells, cortisol-cortisone inter-conversion is catalysed by both a NADP(H)-dependent, bidirectional type 1 11β-hydroxysteroid dehydrogenase (11βHSD1) enzyme and by a NAD+-dependent, unidirectional type 2 11βHSD (11βHSD2) isoenzyme. Since several published studies have reported altered glucocorticoid metabolism in polycystic ovary syndrome (PCOS), the current study assessed whether prenatal exposure of lambs to testosterone propionate...

ea0015oc13 | Reproduction | SFEBES2008

Effect of hypoxia and inflammatory and anti-inflammatory signals on gene expression in sheep ovarian surface epithelium in vitro

Harlow Christopher R , Rae Michael , Price Deborah , McNeilly Alan S , Rhind Stewart M , Hillier Stephen G

The intraperitoneal environment of the ovarian surface epithelium (OSE) has an oxygen (O2) tension of 5–12%. This could impact cellular events leading to ovulation. Furthermore, ovarian cancers arising from the OSE (∼80–90% of ovarian malignancies) are likely to experience increased hypoxia due to the relatively poor vascularisation of solid tumours.Using 1%, 5% or 20% O2, we measured the expression of HSD11B1, COX-2...

ea0028p240 | Pituitary | SFEBES2012

Transcriptional regulation of prolactin in the rat oestrous cycle

Patist Amanda , Featherstone Karen , Spiller David , Semprini Sabrina , McNeilly Judith , McNeilly Alan , Mullins John , White Michael , Davis Julian

Circulating levels of prolactin are subject to acute and long-term regulation by many factors including oestrogen and dopamine. We have studied the regulation of prolactin promoter activity in living pituitary cells using transgenic Fischer rats in which reporter gene expression is regulated by the human prolactin gene locus (hPRL-d2EGFP). We have previously identified pulsatile prolactin transcription patterns in living lactotroph cells in fetal tissue, that became stabilised...

ea0021p298 | Pituitary | SFEBES2009

Onset of prolactin gene transcription in nascent pituitary lactotroph cells

Featherstone Karen , Harper Claire , McNamara Anne , Semprini Sabrina , Spiller David , McNeilly Alan , Mullins John , White Michael , Davis Julian

Pituitary development, in particular the differentiation of anterior pituitary endocrine cells, remains to be fully understood, and may have implications for adult pituitary plasticity and hyperplasia. We have used prolactin-reporter transgenic rats to characterise prolactin transcription during the appearance of newly formed lactotroph cells during fetal development.Transgenic rats with the firefly luciferase reporter gene inserted into exon 1b of a 160...

ea0015oc29 | Pituitary, disease | SFEBES2008

Targeting Cyp11b1 expression in mice to model sequelae of congenital adrenal hyperplasia

Mullins Linda , Peter Audrey , Wrobel Nicola , Aldujaili Emad , McNeilly Judith , Brownstein David , McNeilly Alan , Mullins John , Kenyon Christopher

We have created transgenic mice in which Cyp11b1, the gene encoding 11β-hydroxylase, has been knocked out. Since mice do not secrete adrenal androgens, this knockout line allows a more detailed investigation of phenotypes associated with congenital adrenal hyperplasia (CAH) without the overwhelming virilisation that characterises patients with CAH. Starting with a BAC containing the mouse Cyp11b1/b2 locus and including flanking up- and downstream sequences, a const...

ea0034oc5.5 | Pituitary | SFEBES2014

Spatio-temporal analysis of prolactin gene transcription dynamics reveals short range co-ordination of lactotroph transcription activity in pituitary tissue

Featherstone Karen , Hey Kirsty , Momiji Hiroshi , McNamara Anne , Patist Amanda , Spiller David , Christian Helen , McNeilly Alan , Mullins John , Finkenstadt Barbel , Rand David , White Michael , Davis Julian

Mammalian genes display pulsatile transcription dynamics with bursts of expression occurring with variable duration and frequency. Using the human prolactin gene as a model of tissue specific gene regulation, we have characterised the transcription dynamics of this gene in cell lines, primary cells and pituitary tissue slices. Our data indicate that the tissue environment may have an important influence on cellular transcription activity. Cultures of cells from enzymatically d...

ea0021p25 | Bone | SFEBES2009

Extra-pituitary expression of the prolactin gene in the bone and cartilage of an hPRL-Luc transgenic rat model

Semprini Sabrina , McNeilly Judith R , Ramage Lindsay , Brownstein David G , Salter Donald M , McNeilly Alan , White Michael R H , Davis Julian R E , Mullins John J

Prolactin (PRL) is a peptide hormone produced by the anterior pituitary gland and it is commonly known for its lactogenic and mammotrophic effects. In humans and primates, prolactin is also expressed in extra-pituitary sites where it is associated with numerous biological functions, but its spectrum of expression varies in different species.Hyperprolactinemia-induced osteoporosis is believed to be mediated by hypogonadism, but the expression of the prola...