Searchable abstracts of presentations at key conferences in endocrinology

ea0031p182 | Obesity, diabetes, metabolism and cardiovascular | SFEBES2013

Vitamin B12 and folate imbalance induces cholesterol synthesis and endoplasmic reticulum stress in human adipocytes

Adaikalakoteswari Antonysunil , Moore Jonathan , Ciara Mccarthy , Voyais Philip , Mcternan Philip , Saravanan Ponnusamy , Tripathi Gyanendra

Adipose tissue (AT) plays a central role in integrating energy metabolism and glucose homeostasis. It is the major site of fatty acid storage as triglycerides and is body’s largest cholesterol pool. In AT cholesterol is mostly found in its free, non-esterified form. There is accumulating evidence that cholesterol imbalance in AT is closely associated with adipocyte dysfunction and obesity-mediated metabolic complications, including low levels of high-density lipoprotein c...

ea0038p215 | Obesity, diabetes, metabolism and cardiovascular | SFEBES2015

The role of vitamin B12 deficiency in molecular mechanism of metformin

Khin May Oo , Antonysunil Adaikala , Kumsaiyai Warunee , Voyias Philip , Mcternan Philip , Gates Simon , Tripathi Gyanandra , Saravanan Ponnusamy

The anti-diabetic drug, metformin, is associated with progressive decrease in serum vitamin B12 levels whereas vitamin B12 deficiency is related to increased insulin resistance and dyslipidaemia by altered methylation. As the risk of vitamin B12 deficiency is increased among metformin users, it is of utmost importance to examine how metformin response in vitamin B12 deficient population.We investigated the cellular mechanism of metformin in vitamin B12 i...

ea0059p157 | Obesity & metabolism | SFEBES2018

The impact of lipopolysaccharide on mitochondrial efficiency in brown adipocytes

Omran Farah , McTernan Philip , Christian Mark

Background: The presence of brown adipose tissue (BAT) in adults offers an opportunity to examine inflammatory factors that may affect metabolic function in states of obesity. Gut-derived lipopolysaccharide (LPS), which is elevated in obesity, and initiates the innate immune response in white adipose tissue, has not been fully studied in BAT. The interactions between LPS, TLR4 and ß3-adrenergic receptors in BAT is unknown. ß3-adrenergic receptor ligands as CL 316,243...

ea0077p71 | Metabolism, Obesity and Diabetes | SFEBES2021

Lipopolysaccharide signalling modulates brown fat transcriptome and cytokine secretion

Omran Farah , Murphy Alice , McTernan Philip , Christian Mar

Background: Brown adipose tissue (BAT) thermogenesis offers an appealing prospect to combat obesity. Obesity is characterised by a state of chronic inflammation in adipose tissue mediated by the secretion of a range of inflammatory-cytokines. Our previous work has highlighted that a gut-derived inflammatory agent, lipopolysaccharide (LPS), reduces brown adipocyte activity, insulin sensitivity and mitochondrial-function and is increased with obesity and type 2 diabetes mellitus...

ea0077p175 | Metabolism, Obesity and Diabetes | SFEBES2021

Pressure from pericytes: angiotensin II induced neuropathic pain development as a complication of hypertension

Hardowar Lydia , Sheavyn Matt , McTernan Philip , Bates Dave , Hulse Richard

The renin angiotensin system (RAS) is targeted as means of medical intervention for hypertensive associated complications of stroke, heart failure and obesity. Ultimately, the inhibition of Angiotensin II(ANGII)/Angiotensin II type 1 (AT1) receptor complex has been key in reducing raised blood pressure. Diabetic neuropathic pain (NP) is highly prevalent in the United Kingdom, associated with an increasingly aged population. There is growing acknowledgment that the vascular com...

ea0077p183 | Metabolism, Obesity and Diabetes | SFEBES2021

The influence of metabolic states and a high fat meal on circulating chemerin

Murphy Alice , Dumbell Rebecca , Varma Madhu , Helfer Gisela , McTernan Philip

Background: Chemerin is a multifunctional adipokine involved in pathogenesis of metabolic disease by regulating inflammation, adipocyte plasticity and glucose metabolism. It is known to be influenced by age, adiposity and triglycerides, and supports important roles in systemic lipid and glucose metabolism.However, the direct acute effects of circulating chemerin on varying metabolic disease states given a high–saturated fat meal has not been addressed.<p clas...

ea0086p54 | Metabolism, Obesity and Diabetes | SFEBES2022

Metabolic Endotoxemia impacts mitochondrial function and adipocyte browning in a depot specific manner in Human abdominal adipose tissue

Murphy Alice , Omran Farah , Piya Milan , Christian Mark , McTernan Philip

Introduction: Central obesity is a significant risk factor for type 2 diabetes mellitus (T2DM), with omental adipose tissue (AT) particularly involved in such risk. Additionally, obesity can cause low-level gut-derived endotoxemia, which may drive metabolic dysfunction in AT through mitochondrial damage and reduced BRITE (brown-in-white) adipocytes. Bariatric surgery reduces obesity and may prevent such dysfunction. This study investigated whether endotoxin: 1) impairs mitocho...

ea0086p201 | Metabolism, Obesity and Diabetes | SFEBES2022

Angiotensin II dependent pericyte activation causes neuropathic pain in a type-2 rodent model of diabetes

Hardowar Lydia , McTernan Philip , Bates David , Hulse Richard

Neuropathic pain (NP) is a microvascular complication affecting between 30-50% of people living with diabetes. Current painkillers including non-steroidal pain killers and anti-convulsant are ineffective. At the level of the spinal cord (SC), capillary regression is associated with the onset of NP in diabetic rodent models. Our aim was to investigate angiotensin II mediated pericyte activation in relation to vascular degeneration and the onset of NP. Adult male C57Bl/6J mice w...

ea0086p220 | Metabolism, Obesity and Diabetes | SFEBES2022

Endotoxin Impairs Brown Fat Phenotype and Mitochondrial Function in 2D and 3D Brown Adipocytes Models

Omran Farah , Murphy Alice , McTernan Philip G , Christian Mark

Background: Dysfunctional adipose tissue in obesity is known to contribute to metabolic diseases, including type 2 diabetes mellitus (T2DM). This may be due to increased gut-derived endotoxemia (LPS) reducing brown adipose tissue (BAT) activity and altering mitochondrial function. However, the effect of LPS on BAT activity in 3D culture models has not been studied, despite giving a better representation of in-vivo tissue. Therefore, this study investigated the effects of LPS o...

ea0065p184 | Metabolism and Obesity | SFEBES2019

Vitamin B12 deficiency leads to fatty acid metabolism dysregulation and increased pro-inflammatory cytokine production in human adipocytes and in maternal subcutaneous and omental adipose tissue

Samavat Jinous , Adaikalakoteswari Antonysunil , Boachie Joseph , Jackisch Laura , McTernan Philip , Christian Mark , Saravanan Ponnusamy

Vitamin B12 (B12) is an essential micronutrient required for several metabolic reactions. Animal and clinical studies show that B12-deficiency is associated with metabolic syndrome. Given the key metabolic role of adipose tissue, we investigated whether B12 deficiency may affect triglyceride synthesis and lipid metabolism leading to adipose tissue inflammation. The AbdSc pre-adipocyte cell line (Chub-S7) and human AbdSc primary pre-adipocytes were differentiated under differen...