Searchable abstracts of presentations at key conferences in endocrinology

ea0031p182 | Obesity, diabetes, metabolism and cardiovascular | SFEBES2013

Vitamin B12 and folate imbalance induces cholesterol synthesis and endoplasmic reticulum stress in human adipocytes

Adaikalakoteswari Antonysunil , Moore Jonathan , Ciara Mccarthy , Voyais Philip , Mcternan Philip , Saravanan Ponnusamy , Tripathi Gyanendra

Adipose tissue (AT) plays a central role in integrating energy metabolism and glucose homeostasis. It is the major site of fatty acid storage as triglycerides and is body’s largest cholesterol pool. In AT cholesterol is mostly found in its free, non-esterified form. There is accumulating evidence that cholesterol imbalance in AT is closely associated with adipocyte dysfunction and obesity-mediated metabolic complications, including low levels of high-density lipoprotein c...

ea0038p215 | Obesity, diabetes, metabolism and cardiovascular | SFEBES2015

The role of vitamin B12 deficiency in molecular mechanism of metformin

Khin May Oo , Antonysunil Adaikala , Kumsaiyai Warunee , Voyias Philip , Mcternan Philip , Gates Simon , Tripathi Gyanandra , Saravanan Ponnusamy

The anti-diabetic drug, metformin, is associated with progressive decrease in serum vitamin B12 levels whereas vitamin B12 deficiency is related to increased insulin resistance and dyslipidaemia by altered methylation. As the risk of vitamin B12 deficiency is increased among metformin users, it is of utmost importance to examine how metformin response in vitamin B12 deficient population.We investigated the cellular mechanism of metformin in vitamin B12 i...

ea0059p157 | Obesity & metabolism | SFEBES2018

The impact of lipopolysaccharide on mitochondrial efficiency in brown adipocytes

Omran Farah , McTernan Philip , Christian Mark

Background: The presence of brown adipose tissue (BAT) in adults offers an opportunity to examine inflammatory factors that may affect metabolic function in states of obesity. Gut-derived lipopolysaccharide (LPS), which is elevated in obesity, and initiates the innate immune response in white adipose tissue, has not been fully studied in BAT. The interactions between LPS, TLR4 and ß3-adrenergic receptors in BAT is unknown. ß3-adrenergic receptor ligands as CL 316,243...

ea0077p71 | Metabolism, Obesity and Diabetes | SFEBES2021

Lipopolysaccharide signalling modulates brown fat transcriptome and cytokine secretion

Omran Farah , Murphy Alice , McTernan Philip , Christian Mar

Background: Brown adipose tissue (BAT) thermogenesis offers an appealing prospect to combat obesity. Obesity is characterised by a state of chronic inflammation in adipose tissue mediated by the secretion of a range of inflammatory-cytokines. Our previous work has highlighted that a gut-derived inflammatory agent, lipopolysaccharide (LPS), reduces brown adipocyte activity, insulin sensitivity and mitochondrial-function and is increased with obesity and type 2 diabetes mellitus...

ea0077p175 | Metabolism, Obesity and Diabetes | SFEBES2021

Pressure from pericytes: angiotensin II induced neuropathic pain development as a complication of hypertension

Hardowar Lydia , Sheavyn Matt , McTernan Philip , Bates Dave , Hulse Richard

The renin angiotensin system (RAS) is targeted as means of medical intervention for hypertensive associated complications of stroke, heart failure and obesity. Ultimately, the inhibition of Angiotensin II(ANGII)/Angiotensin II type 1 (AT1) receptor complex has been key in reducing raised blood pressure. Diabetic neuropathic pain (NP) is highly prevalent in the United Kingdom, associated with an increasingly aged population. There is growing acknowledgment that the vascular com...

ea0077p183 | Metabolism, Obesity and Diabetes | SFEBES2021

The influence of metabolic states and a high fat meal on circulating chemerin

Murphy Alice , Dumbell Rebecca , Varma Madhu , Helfer Gisela , McTernan Philip

Background: Chemerin is a multifunctional adipokine involved in pathogenesis of metabolic disease by regulating inflammation, adipocyte plasticity and glucose metabolism. It is known to be influenced by age, adiposity and triglycerides, and supports important roles in systemic lipid and glucose metabolism.However, the direct acute effects of circulating chemerin on varying metabolic disease states given a high–saturated fat meal has not been addressed.<p clas...

ea0065p184 | Metabolism and Obesity | SFEBES2019

Vitamin B12 deficiency leads to fatty acid metabolism dysregulation and increased pro-inflammatory cytokine production in human adipocytes and in maternal subcutaneous and omental adipose tissue

Samavat Jinous , Adaikalakoteswari Antonysunil , Boachie Joseph , Jackisch Laura , McTernan Philip , Christian Mark , Saravanan Ponnusamy

Vitamin B12 (B12) is an essential micronutrient required for several metabolic reactions. Animal and clinical studies show that B12-deficiency is associated with metabolic syndrome. Given the key metabolic role of adipose tissue, we investigated whether B12 deficiency may affect triglyceride synthesis and lipid metabolism leading to adipose tissue inflammation. The AbdSc pre-adipocyte cell line (Chub-S7) and human AbdSc primary pre-adipocytes were differentiated under differen...

ea0065p185 | Metabolism and Obesity | SFEBES2019

Endotoxin and adiposity as mediators of down-regulating the BRITE fat phenotype

Omran Farah , Murphy Alice , Jackisch Laura , Samavat Jinus , McTernan Philip G , Christian Mark

Background: The acquisition of brown-adipocyte-properties by white-adipocytes (BRITE-adipocytes) is an appealing-prospect to combat obesity and type-2-diabetes-Mellitus (T2DM). This may support counteracting the impact of inflammation and mitochondrial-dysfunction, which contribute to the obesity-pathogenesis. However, our previous-finding shave shown that the gut-derived-inflammatory-agent endotoxin can increase the inflammatory-response in white-adipose-tissue impacted by ob...

ea0065p202 | Metabolism and Obesity | SFEBES2019

Endoplasmic reticulum stress directly impacts mitochondrial function in human adipocytes

Jackisch Laura , Murphy Alice , Kumar Sudhesh , Randeva Harpal , Tripathi Gyanendra , McTernan Philip

Background: Dysfunctional endoplasmic reticula (ER) and mitochondria contribute to the pathogenesis of obesity and type 2 diabetes mellitus (T2DM). This may, in part, be facilitated by cross-talk between the two organelles during conditions of nutrient excess such as obesity, however the potential impact of ER stress on mitochondrial function has not been well studied. This study investigated whether induction of ER stress in human adipocytes may contribute to mitochondrial dy...

ea0059p168 | Obesity &amp; metabolism | SFEBES2018

The impact of freeze dried broccoli extract to mitigate inflammation in human adipocytes through the mevalonate pathway

Murphy Alice , Azharian Sahar , Tripathi Gyanendra , Barker Guy , Chappell Michael , McTernan Philip G

Background: Delivery of nutrient excess in obesity can disrupt protein folding in the endoplasmic reticulum (ER) within adipose tissue; this activates the unfolded protein response (UPR) and contributes to type 2 diabetes mellitus (T2DM) risk. Thus, the aims of this study were to utilise freeze-dried broccoli extract (BE) as a nutrient to mitigate such cellular damage in human adipocytes, understand the relevance of associated pathways, and create a mathematical model of the U...