Searchable abstracts of presentations at key conferences in endocrinology

ea0063p711 | Pituitary and Neuroendocrinology 2 | ECE2019

Whole genome demethylation status of somatic DNA extracted from different pituitary adenoma types

Szabo Borbala , Nemeth Kinga , Meszaros Katalin , Szucs Nikolett , Czirjak Sandor , Reiniger Lilla , Patocs Attila , Butz Henriett

Background: Although the role of hypermethylation of certain tumor suppressor genes are known in pituitary adenomas little information is available regarding whole methylation-demethylation status and especially regarding its correlation with clinical parameters. High-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) is an easy and accurate method to detect the level of methylcytosine (5 mC) and the demethylation intermedier hydroxymethylcytosine (5 hmC) ...

ea0081ep691 | Pituitary and Neuroendocrinology | ECE2022

Genomic and epigenomic aspects of Aspirin antitumoral effect in pituitary adenoma

Szabo Borbala , Krokker Lilla , Meszaros Katalin , Saskői Eva , Nemeth Krisztina , Szabo Pal , Szucs Nikolette , Czirjak Sandor , Patocs Attila , Butz Henriett

Background: In our previous works the interdependence of DNA demethylation with proliferation and differentiation of pituitary neuroendocrine tumours (PitNET) and the inhibitory effect of Aspirin on pituitary cell proliferation were demonstrated. Although the role of Aspirin in epigenetic regulation was described in other malignancies, its correlation with pituitary tumorigenesis is currently unknown.Our objective: was to investigate the genomic and epig...

ea0063gp6 | Adrenal and Neuroendocrine - Tumour | ECE2019

Cellular modelling of SDH-associated pathomechanism of pheochromocytomas and paragangliomas

Sarkadi Balazs , Meszaros Katalin , Krencz Ildiko , Zakarias Sara , Nemeth Kinga , Barja Gabor , Sebestyen Anna , Papay Judit , Borka Katalin , Hujber Zoltan , Toth Miklos , Igaz Peter , Chinopoulos Christos , Patocs Attila

Introduction: The capability of cancer to accommodate to special metabolic circumstances is a hallmark of itÂ’s existence. Pheochromocytoma/paragangliomas (Pheo/PGL) are rare neuroendocrine tumors with strong and specific metabolic phenotype due to mutations of genes encoding succinate dehydrogenase (SDH) subunits. In this study our aim was to evaluate the expression of glutaminase-1 in hereditary Pheo/PGL tissues and to inhibit SDH activity via pharmacological treatments ...

ea0063p444 | Adrenal and Neuroendocrine Tumours 2 | ECE2019

The Arg244His missense mutation in SDHB-1 leads to altered metabolism in Caenorhabditis elegans: a new disease model

Saskoi Eva , Hujber Zoltan , Liko Istvan , Meszaros Katalin , Sarkadi Balazs , Matyasi Barbara , Kovacs Attila L , Patthy Laszlo , Nyiro Gabor , Svab Gergely , Tretter Laszlo , Mehta Anil , Sebestyen Anna , Patocs Attila , Takacs-Vellai Krisztina

The succinate dehydrogenase (SDH) enzyme complex consisting of four subunits (SDHA, SDHB, SDHC and SDHD) has a dual function in the process of mitochondrial energy generation. It converts succinate to fumarate as part of the TCA cycle and also transfers electrons to ubiquinone as part of the electron transport chain. Mutation in any subunit of the enzyme complex increases the risk for the development of neuroendocrine tumors including paraganglioma (PGL) and pheochromocytoma (...

ea0070oc1.1 | Adrenal and Cardiovascular Endocrinology | ECE2020

Inhibition of glutaminases as a potential novel treatment for SDHB-associated pheochromocytomas/paragangliomas

Sarkadi Balázs , Katalin Meszaros , Krencz Ildiko , Canu Letizia , Krokker Lilla , Zakarias Sara , Barna Gabor , Sebestyen Anna , Papay Judit , Hujber Zoltan , Butz Henriett , Darvasi Otto , Igaz Peter , Doczi Judit , Luconi Michaela , Chinopoulos Christos , Patocs Attila

Pheochromocytoma/paragangliomas (Pheo/PGL) are rare neuroendocrine cancers with strong genetic background. Mutations in the SDHB subunit of succinate dehydrogenase (SDH) predispose to malignant disease with limited therapeutic options and poor prognosis. Novel prognostic markers and therapeutic targets are required to decrease the high mortality rate. Glutaminases play a crucial role in the metabolism of SDH impaired tumor cells. By using a host of cellular and molecu...