Searchable abstracts of presentations at key conferences in endocrinology

ea0065p248 | Metabolism and Obesity | SFEBES2019

Using problem-based learning (PBL) to engage undergraduate medical students in endocrinology at Edinburgh Medical School

Pender Frederic T , Morley Steven D

Background: Endocrinology, taught in Year 2 of the Edinburgh undergraduate MBChB curriculum to approximately 200 medical students, can present a complex challenge and a test of engagement. Core biomedical curriculum content is delivered as large group teaching (lectures) and then revisited in smaller groups (clinical-cased based tutorials, PBL) designed to integrate learning by applying basic knowledge in a clinical context. PBL first implemented in the 1960s at McMaster Unive...

ea0013p133 | Growth and development | SFEBES2007

The mosaic pattern of 21-OHase/LacZ transgene expression changes during organogenesis and growth of the adrenal cortex

Chang Su-Ping , West John , Morley Steven

The mouse steroid 21-hydroxylase-A gene promoter (21-OH) directs adrenal cortex-specific expression of the β-galactosidase reporter in 21-OHase/LacZ transgenic mice. Multiple independent 21-OHase/LacZ transgenic lines show a similar mosaic pattern of reporter expression probably reflecting stochastic transgene silencing. In the adult, the mosaic reporter expression occurs as radial stripes spanning the adrenal cortex. A similar radial striped pattern occurs ...

ea0034p336 | Reproduction | SFEBES2014

Elevation of HRPE773 (ZG16B) expression in amnion at term and in human ectocervical cell lines treated with inflammatory mediators is consistent with a function in innate immunity

Hayhurst Rachel Rachel , Hibbert Nanette , Frew Lorraine , Stock Sarah , Riley Simon , Morley Steven

Introduction: Labour is an inflammatory process involving the innate immune system, premature activation of which, for example by infection, can lead to preterm birth before 37 weeks of gestation with profound pathophysiological consequences for the offspring. The putative antimicrobial protein HRPE773 (ZG16B) expressed in human female reproductive secretory epithelia, has been shown previously to be elevated in human endometrium during the menstrual early secretory phase and ...

ea0028p290 | Reproduction | SFEBES2012

Uterine cell turnover in a mouse model of adenomyosis caused by congenital adrenal hyperplasia

Sadraie Maryam , Hastings Julie , Mullins Linda , Mullins John , Morley Steven , Kenyon Christopher

We have investigated cell proliferation and apoptosis in Cyp11b1 null (ko) mice. Reduced glucocorticoid (corticosterone) synthesis in ko mice is associated with increased progesterone and deoxycorticosterone and the development of adenomyosis. Numbers of putative uterine stem cells were assessed in wild type (wt) and ko at an age (10–12 months) when the onset of adenomyosis was considered imminent. Mice were infused with bromodeoxyuridine (BrdU) for seven days then killed...

ea0028p312 | Steroids | SFEBES2012

Effects of In-Utero Exposure to Endocrine Disruptors on the Ovine Fetal Adrenal Gland

Buckley Charlotte , Danga Mercy , Hogg Kirsten , Amezaga Maria , Rhind Stewart , Fowler Paul , Rae Michael , Morley Steven

Introduction: The repercussions of long-term exposure to low level mixtures of endocrine disrupting compounds (EDCs) which are present, ubiquitously, in the environment are not well understood. Heat-treated sewage sludge, an EDC-rich product used as fertiliser, has been shown to compromise testicular and ovarian development. However, to date, there has been limited investigation in exposed animals of the adrenal glands, the steroid hormone products of which play key roles in f...

ea0025p281 | Reproduction | SFEBES2011

HRPE773 (ZG16B) expression is elevated in human endometrium during the early secretory phase of the menstrual cycle and in uterine decidua following miscarriage

Ng Bonnie , McDonald Sarah , Ren Xia , Mullins John , Rae Michael , Critchley Hillary , Horne Andrew , Morley Steven

Introduction: Expression of murine CSP-1/Dcpp secretory proteins was first identified in sublingual salivary glands and subsequently in secretory epithelia of several other tissues, including the mouse female reproductive tract where expression is regulated by oestrogen. Preliminary studies indicated that HRPE773, the human CSP-1/Dcpp orthologue, displayed a similar pattern of expression to its murine counterparts. We therefore hypothesized that HRPE773 might be expressed in h...

ea0021p172 | Diabetes and metabolism | SFEBES2009

Aldosterone production by the mouse adrenal gland is compromised by a high fat diet and 11β-hydroxysteroid dehydrogenase type 1 deficiency

Nilsson Frida , Morrison Hamish , Ramage Lynne , Morton Nicholas , Seckl Jonathan , Morley Steven , Kenyon Christopher

Deficiency of the intracellular glucocorticoid (GC) reactivating enzyme 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) invokes compensatory activation of the HPA axis. Nevertheless, 11β-HSD1−/− mice resist dietary-induced obesity indicating that target tissues are protected from GC actions. Since endocrine adaptation to diet is mediated by altered HPA activity, we investigated whether the adrenal response to high fat (HF) diet is compr...

ea0021p351 | Steroids | SFEBES2009

Responses of putative adrenocortical stem/progenitor cells and transiently amplifying cells to ACTH

Morrison Hamish , Nilsson Frida , Chang Su-Ping , West John , Kenyon Christopher , Morley Steven

Steroidogenic cells of the adrenal cortex are thought to originate from a peripherally-located self-renewing population of undifferentiated stem or progenitor cells. These cells divide infrequently to give daughters, which proliferate transiently (termed transiently amplifying (TA) cells), migrate and differentiate to replenish the functionally-differentiated zones of the adrenal cortex, or remain in situ as undifferentiated stem cells. Theoretically such stem cells can...

ea0059p038 | Adrenal and steroids | SFEBES2018

QRT-PCR analysis of the effect of in utero exposure to sewage sludge on steroidogenic gene expression in ovine foetal adrenal gland

Cooper Erin A , Reddy Sreedath , Allenson Abbie Z , Cooper Duncan P , Fowler Paul A , Rae Michael T , Morley Steven D

Endocrine disruptors are chemicals which in low concentrations can disturb gene expression in a range of endocrine glands and organs including the fetal and adult adrenal glands, potentially resulting in altered steroidogenic flux. With exposure to endocrine disruptors affecting both animals and humans, it is important to assess both the mechanisms and consequences of disruption in steroidogenic pathways, particularly as foetal development may be especially sensitive to endocr...