Searchable abstracts of presentations at key conferences in endocrinology

ea0028p290 | Reproduction | SFEBES2012

Uterine cell turnover in a mouse model of adenomyosis caused by congenital adrenal hyperplasia

Sadraie Maryam , Hastings Julie , Mullins Linda , Mullins John , Morley Steven , Kenyon Christopher

We have investigated cell proliferation and apoptosis in Cyp11b1 null (ko) mice. Reduced glucocorticoid (corticosterone) synthesis in ko mice is associated with increased progesterone and deoxycorticosterone and the development of adenomyosis. Numbers of putative uterine stem cells were assessed in wild type (wt) and ko at an age (10–12 months) when the onset of adenomyosis was considered imminent. Mice were infused with bromodeoxyuridine (BrdU) for seven days then killed...

ea0013p191 | Diabetes, metabolism and cardiovascular | SFEBES2007

Renal effects of ACTH: functional and microarray studies in the mouse

Kenyon Christopher , Mullins Linda , Dunbar Donald , Mullins John , Bailey Matthew

We investigated ACTH-induced hypertension in mice by studying renal function in vivo and by analysing renal gene expression by microarray and RT PCR methods. During two weeks sc infusion with Synacthen, mean blood pressure in adult male mice increased (89±5 vs 110±2 mmHg), and plasma corticosterone, adrenal weights and drinking rate increased by 5, 2 and 2.5-fold respectively (P<0.01); renal mass was unaffected. Greater mineralocorticoid activity wa...

ea0028oc4.6 | Steroid | SFEBES2012

Fatty liver in a mouse model of congenital adrenal hyperplasia is driven by peroxisome proliferator-activated receptors

McInnes Kerry , Gallacher Stuart , Caughey Sarah , Carter Rod , Yau Joyce , Mullins Linda , Kenyon Chris

Mice with congenital adrenal hyperplasia due to a null mutation of cyp11b1, the gene encoding the steroidogenic enzyme 11β-hydroxylase, have low glucocorticoid levels, reduced adiposity and increased liver weight. It is notable that null mice are also glucose intolerant. Here, we have investigated these phenotypes in more detail. Plasma corticosterone levels were less responsive to restraint stress and cholesterol and triglyceride levels were not significantly different i...

ea0015oc29 | Pituitary, disease | SFEBES2008

Targeting Cyp11b1 expression in mice to model sequelae of congenital adrenal hyperplasia

Mullins Linda , Peter Audrey , Wrobel Nicola , Aldujaili Emad , McNeilly Judith , Brownstein David , McNeilly Alan , Mullins John , Kenyon Christopher

We have created transgenic mice in which Cyp11b1, the gene encoding 11β-hydroxylase, has been knocked out. Since mice do not secrete adrenal androgens, this knockout line allows a more detailed investigation of phenotypes associated with congenital adrenal hyperplasia (CAH) without the overwhelming virilisation that characterises patients with CAH. Starting with a BAC containing the mouse Cyp11b1/b2 locus and including flanking up- and downstream sequences, a const...