Searchable abstracts of presentations at key conferences in endocrinology

ea0014p623 | (1) | ECE2007

Differential effects of two-week treatment with atorvastatin or elocalcitol, two RhoA/ROK signalling modulators, on erectile function and sildenafil responsiveness in spontaneously hypertensive rats

Morelli Annamaria , Zhang Xin-Hua , Filippi Sandra , Vignozzi Linda , Mungai Sara , Adorini Luciano

Increased RhoA/Rho-kinase (ROK) signalling is known to impair erectile function. Spontaneously hypertensive rats (SHR) over-express penile RhoA and show an impaired erectile response. We tested treatments known to inhibit RhoA activation, on erectile function and sildenafil responsiveness in SHR. SHR have been treated for two weeks with atorvastatin (5 and 30 mg/Kg/day), or with elocalcitol (30 μg/Kg/day), a vitamin D receptor (VDR) agonist. The normotensive Wistar Kyoto ...

ea0014oc7.5 | Reproductive endocrinology I | ECE2007

Estrogens regulate epididymal contractility through RhoA/Rho-kinase signaling

Filippi Sandra , Morelli Annamaria , Vignozzi Linda , Mancina Rosa , Mungai Sara , Ambrosini Stefano , Vannelli Gabriella Barbara , Forti Gianni , Maggi Mario

Epididymis (epi) is a sex steroid-sensitive duct provided with spontaneous motility, allowing sperm transport. We previously demonstrated that human epi expresses a high abundance of mRNA for ER-alpha and ER-beta. We demonstrated that in epi estrogens up-regulate either oxytocin (OT) responsiveness, acting at the receptor level, and responsiveness to endothelin-1 (ET-1), another well-known stimulator of epididymal motility. However, we did not find any significant change eithe...

ea0014oc11.3 | Reproductive endocrinology II | ECE2007

Sex steroid and leptin regulation of KISS1/GPR54 system, a new regulator of the neuroendocrine reproductive axis, in human fetal GnRH-secreting neurons

Morelli Annamaria , Marini Mirca , Mancina Rosa , Vignozzi Linda , Pezzatini Anna , Luconi Michaela , Mungai Sara , Fibbi Benedetta , Filippi Sandra , Barbara Vannelli Gabriella , Forti Gianni , Maggi Mario

The molecular mechanisms underlying the reawakening of hypothalamic GnRH neurons at puberty remain to be elucidated. Recently, the G protein-coupled receptor 54 (GPR54) and its endogenous ligand kisspeptin, encoded by the KISS1 gene, have been involved. In fact, GPR54 mutations cause idiopathic hypogonadotropic hypogonadism in human and mice. We used the previously characterized primary culture of human fetal olfactory GnRH-secreting neurons, FNC-B4, to study in vitro t...

ea0014p624 | (1) | ECE2007

Testosterone regulates RhoA/Rho-kinase signalling in two distinct animal models of chemical diabetes

Vignozzi Linda , Morelli Annamaria , Filippi Sandra , Ambrosini Stefano , Mancina Rosa , Luconi Michaela , Mungai Sara , Barbara Vannelli Gabriella , Zhang Xin-Hua , Forti Gianni , Maggi Mario

The contractile RhoA/Rho-kinase (Rock) signalling pathway is up-regulated in penile tissue in animal models of experimental diabetes and has been proposed to contribute to diabetes-related erectile dysfunction (ED). In previous studies we demonstrated that testosterone (T) restores diabetes-induced ED by influencing the NO/cGMP/PDE5 pathway.AimTo investigate the effect of T on the RhoA/Rock signalling in course of diabetes.MethodsWe used two distinct animal models of chemical ...