Searchable abstracts of presentations at key conferences in endocrinology

ea0084ps1-02-18 | Hypothyroidism | ETA2022

Human cerebral organoids as in vitro platform to assess thyroid hormone system disrupting chemicals

Fernandez-Vallone Valeria , Hellwig Lina , Opitz Robert , Obermayer-Wasserscheid Benedikt , Wiese Niklas , Rijntjes Eddy , Kohrle Josef , Mergenthaler Philipp , Stachelscheid Harald

Thyroid hormones (TH) play a fundamental role in brain development. Changes on TH availability during pregnancy and early childhood can lead to neurological disorders. Accordingly, concerns are mounting that exposure to environmental compounds capable of interfering with TH action can disrupt neuronal development. Current approaches to categorize chemicals as thyroid hormone system disrupting chemicals (THSDC) are mainly based on determination of changes in circulating TH conc...

ea0084ps2-09-81 | Thyroid Hormone ACTION | ETA2022

An experimental framework for probing molecular mechanisms of local thyroid hormone action during cortical neurogenesis in human cerebral organoids

Amber Julie Bresser Audrey , Graffunder Adina , Anthofer Larissa , Megges Matthias , Obermayer-Wasserscheid Benedikt , Rijntjes Eddy , Wiese Niklas , Fernandez-Vallone Valeria , Stachelscheid Harald , Kuhnen Peter , Opitz Robert

Recently, human cerebral organoids (hCOs) emerged as powerful three-dimensional in vitro models recapitulating key aspects of early human cerebral cortex development. Improved protocols for hCO generation from induced pluripotent stem cells (hiPSC) paved the way for new disease modeling approaches and enable multimodal analyses of perturbed neurogenesis resulting from manipulation of signaling pathways or gene function. These prospects prompted us to adopt this techno...

ea0073oc5.1 | Oral Communications 5: Thyroid | ECE2021

Identification of iodothyronine deiodinase 2 inhibitors among FDA-approved drug library using high throughput screening

Frädrich Caroline , Renko Kostja , Wiese Niklas , Fischbach Anja , Seyffarth Carola , Neuenschwander Martin , von Kries Jens Peter , Köhrle Josef

BackgroundDevelopment and application of novel drugs frequently fail due to adverse changes of TH-levels or thyroid histology in animal experiments. Such effects potentially arise from interference with thyroid gland function or key regulators of local thyroid hormone (TH) transport, metabolism and action. Deiodinases are key enzymes in TH in-/activation, with impact on development, cell differentiation and energy metabolism among other physiological pro...

ea0073pep10.1 | Presented ePosters 10: Thyroid | ECE2021

A semi-automatic, non-radioactive 384-well high throughput screening DIO2 assay

Frädrich Caroline , Renko Kostja , Wiese Niklas , Fischbach Anja , Seyffarth Carola , Neuenschwander Martin , von Kries Jens Peter , Köhrle Josef

Thyroid hormone (TH) homeostasis depends on the coordination of several key events to maintain proper local TH signaling, including iodide uptake, hormone synthesis, metabolism, and elimination. The three isoenzymes, Deiodinases (DIO1-3), are essential components of hormone metabolism. They catalyze iodide release the outer and/or inner ring of TH structure to convert between their active and inactive forms. The activity of DIOs has been identified as an important endpoint reg...