Searchable abstracts of presentations at key conferences in endocrinology

ea0038ecp1.1 | (1) | SFEBES2015

The physiology and pharmacology of the fasting-induced hormone, FGF21

Owen Bryn

The ability to adapt to changing environmental stimuli, such as the availability of food, is essential for the survival of species. As such, complex mechanisms have evolved to maintain metabolic homeostasis during periods of nutritional challenge. In 2007, the Fibroblast Growth Factor 21 (FGF21) was identified as a nuclear receptor-regulated hepatokine that is induced during starvation. Since then, considerable effort has been devoted to elucidate the breadth of actions of FGF...

ea0077oc2.3 | Endocrine Cancer and Late Effects | SFEBES2021

A novel in vivo platform for studying tumour vascularization and endocrine responses

Lopes Tatiana , Prague Julia , Salem Victoria , Owen Bryn

Background: Tumour growth is critically dependent on blood perfusion, its source of oxygen and nutrients. Therefore, tumour vascularization has become an attractive target for the treatment of many cancers types. The study of endocrine-responsive tumours, in particular, needs improved platforms to screen drugs targeting vascularization that have better resolution and that do not compromise on inter-individual variability.Aim: Hence, we’ve set out to...

ea0065p220 | Metabolism and Obesity | SFEBES2019

GLP-1/Glucagon dual agonist affects amino acid metabolism

Scott Rebecca , Hope David , Owen Bryn , Bloom Steve , Tan Tricia

Background: GLP-1/glucagon dual agonists are being developed as treatments for obesity due to their combined effect of reducing food intake while increase energy expenditure. Though the effect of the dual agonist on carbohydrate and lipid metabolism is well studied, little is known about the effects on protein metabolism. This study aimed to examine the acute and chronic effects of the GLP-1/glucagon dual agonist on amino acid metabolism.Methods: The lon...

ea0065p177 | Metabolism and Obesity | SFEBES2019

Investigating the role of GPR119 in the vagus nerve

Cheng Sijing , Roberts Anna , Norton Mariana , Owen Bryn , Hanyaloglu Aylin , Murphy Kevin

The prevalence of obesity and its associated metabolic diseases are increasing, but current treatments are ineffective or impractical. Understanding how the gut–brain axis senses nutrients to regulate appetite and glucose homeostasis may identify new drug targets and treatments. The G protein-coupled receptor 119 (GPR119) has several endogenous lipid ligands and has been proposed to act as a nutrient sensor in the gastrointestinal tract. GPR119 is expressed on enteroendoc...

ea0059p198 | Reproduction | SFEBES2018

Dax1 controls female fertility as a hypothalamic rheostat of estrogen receptor-alpha

Fernandes Freitas Isabel , Manchishi Stephen , Colledge William , Dhillo Waljit , Owen Bryn

Coupling the release of pituitary hormones to the developmental stage of the oocyte is essential for female fertility. It requires estrogen to have simultaneous positive and negative feedback effects on spatially-distinct regions of the hypothalamus. However, the mechanistic basis for this differential effect is not known. We have found that negative-feedback is mediated by the nuclear receptor Dax1, which is present in the arcuate hypothalamic nucleus and serves as a ligand-d...

ea0077oc3.5 | Metabolism, Obesity and Diabetes | SFEBES2021

Microbial tryptophan metabolites modulate L-cell induced GLP-1 secretion to improve glucose homeostasis

Phuah Phyllis , Cheng Sijing , Norton Mariana , Roberts Anna , Otsubo Emile , Gribble Fiona , Reimann Frank , Hanyaloglu Aylin , Owen Bryn , Murphy Kevin

Growing evidence implicates gut microbiota-derived metabolites in metabolic homeostasis. Gut microbial dysbiosis occurs in obesity, while high-fibre and high-protein diets, which improve glucose tolerance and induce weight loss, are associated with the generation of microbial metabolites. Understanding how the gut responds to microbial metabolites may identify mechanisms that induce satiety and improve glucoregulation, revealing novel therapeutic targets. Indole is generated f...

ea0028p168 | Obesity, diabetes, metabolism and cardiovascular | SFEBES2012

Cholestatic pregnancy has a gender-specific programming effect in mouse adult offspring

Papacleovoulou Georgia , Abu-Hayyeh Shadi , Nikolopoulou Evanthia , Owen Bryn , Nikolova Vanya , Jansen Eugene , Knisely Alex , Williamson Catherine

Alterations in maternal metabolism can programme disease in the offspring. Intrahepatic cholestasis of pregnancy is a liver disease of pregnancy that presents with increased maternal serum bile acids (BA) and dyslipidaemia. We aimed to investigate whether cholestatic pregnancy affects the subsequent health of the offspring. We developed a cholestatic mouse model by supplementation of a normal chow (NC) diet with 0.5% cholic acid during pregnancy. 18 weeks old female and male o...

ea0077p41 | Metabolism, Obesity and Diabetes | SFEBES2021

Investigating 2-oleoylglycerol responsive neuronal pathways

Cheng Sijing , Norton Mariana , Roberts Anna , Martin Alonso Aldara , Phuah Phyllis , Tulloch Emily , Vinette Heloise , Ng Kaa-Yung , Owen Bryn , Hanyaloglu Aylin , Murphy Kevin

Dietary fat intake is an important source of energy and in excess can drive the development of obesity. Investigating how dietary fat intake alters neuronal activity in the brain and drives behavioural changes may help us understand the mechanisms behind high fat diet induced obesity. 2-oleoyglycerol (2-OG) is a naturally occurring unsaturated long chain fatty acid produced by fat digestion in the gut and a ligand of G protein-coupled receptor 119 (GPR119). Published data has ...

ea0077p243 | Reproductive Endocrinology | SFEBES2021

The effect of exogenous kisspeptin administration in a novel mouse model of hypothalamic amenorrhoea

Shrewsbury Jed V , Ng Kah-Yan , McIntyre Caitlin , Li Xiao Feng , Phylactou Maria , O’Byrne Kevin T , Abbara Ali , Dhillo Waljit S , Owen Bryn M

Kisspeptin is integral to hypothalamo-pituitary-gonadal (HPG) axis function and overall fertility. Functional deficiency of GnRH/lH secretion in the central reproductive disease, hypothalamic amenorrhoea (HA), indicates diminished kisspeptin signalling. Clinical trials have shown kisspeptin to be a viable therapeutic intervention. However, repeated administration led to the development of tachyphylaxis and so is likely a problem for future implementation. Indeed, elevated LH r...

ea0065oc6.6 | Reproductive Endocrinology and Biology | SFEBES2019

Kisspeptin enhances the brain processing of attraction in men

Yang Lisa , Demetriou Lysia , Wall Matt , Mills Edouard , Zargaran David , Sykes Mark , Prague Julia , Abbara Ali , Owen Bryn , Bassett Paul , Rabiner Eugenii , Comninos Alexander , Dhillo Waljit

Background: Successful reproduction relies on integration of sensory cues of attraction with corresponding emotions and behaviours. However, the intrinsic factors integrating these fundamental aspects of human attraction with limbic and reproductive pathways have not been fully identified. Kisspeptin is a crucial activator of the reproductive axis and together with its receptor is widely expressed throughout the limbic and olfactory systems in humans suggesting a potential rol...