Searchable abstracts of presentations at key conferences in endocrinology

ea0081p614 | Diabetes, Obesity, Metabolism and Nutrition | ECE2022

Dopaminergic system and adipose tissue: in vitro effect of cabergoline on white, brown and beige adipogenesis and lipogenesis processes

Negri Mariarosaria , Pivonello Claudia , Amatrudo Feliciana , Patalano Roberta , Monto Tatiana , Angelis Cristina de , Simeoli Chiara , Simona Auriemma Renata , Colao Annamaria , Pivonello Rosario

Adipose tissue is an endocrine organ releasing adipokines and expressing specific markers and intracellular mediators, which regulate whole-body energy by balancing lipid accumulation and utilization through adipogenesis, the differentiation of preadipocytes into mature adipocytes associated with gradual lipid storage, and lipogenesis, the additional lipid accumulation in mature adipocytes. Adipogenesis and lipogenesis, strongly induced by insulin, occur in white adipocytes, s...

ea0063p1020 | Interdisciplinary Endocrinology 2 | ECE2019

Circulating miRNAs expression profile in in vitro muscle cell model exposed to cortisol excess

Patalano Roberta , Pivonello Claudia , Monto Tatiana , Simeoli Chiara , Negri Mariarosaria , Di Gennaro Gilda , Papa Fortuna , de Angelis Cristina , Cristina De Martino Maria , Pivonello Rosario

Cushing’s Syndrome (CS) is associated with a severe myopathy mainly affecting type 2 muscle fibres, with a higher prevalence in male subjects. The excess of glucocorticoids (GC) levels represents the most common cause of myopathy due to an alteration of protein metabolism. The non-coding post-transcriptional gene regulators muscle-specific microRNAs (miRNA) control muscle development and differentiation but when dysregulated lead to skeletal muscle (SM) disorder. The aim ...

ea0049ep927 | Pituitary - Basic | ECE2017

Ketoconazole induces inhibition of cell viability and apoptosis in an ACTH-secreting tumour cell line model

Patalano Roberta , Pivonello Claudia , Solari Domenico , Vitulli Francesca , Iacuaniello Davide , Leo Monica De , Negri Mariarosaria , Provvisiero Donatella Paola , Cavallo Luigi Maria , Cappabianca Paolo , Colpo Annamaria , Pivonello Rosario

Chronic cortisol excess as a consequence of ACTH overproduction from a pituitary tumour is responsible for the development of Cushing’s disease (CD). The first-line treatment for CD is pituitary surgery, but medical treatment is an alternative second-line approach to control cortisol excess. Among pharmacological agents, the adrenal-blocking drug ketoconazole (KT), is able to control cortisol excess in the majority of patients with CD. During KT treatment, the adrenal blo...

ea0041ep599 | Endocrine tumours and neoplasia | ECE2016

Dual targeting of PIK3/AKT/mTOR and IGF1/KRAS/BRAF pathways in an in vitro model of ovarian cancer: strategies for cell proliferation control

de Angelis Cristina , Galdiero Giacomo , Pivonello Claudia , Patalano Roberta , Bifulco Giuseppe , Di Spiezio Sardo Attilio , Alviggi Carlo , De Placido Giuseppe , Colao Annamaria , Pivonello Rosario

Ovarian cancer (OC) is the most lethal gynecological cancer. Debulking surgery and platinum-based chemotherapy are the cornerstone of OC management; however, after a partial initial response, tumors invariably relapse. Therapeutic approaches should account for interindividual heterogeneity since OC histiotypes show distinct genetic profile. A2780 cell line has been annotated as high grade serous ovarian cancer (HGSOC); nevertheless, recent research underlined that the genetic ...

ea0041ep620 | Endocrine tumours and neoplasia | ECE2016

Potential role of the adrenolitic drug mitotane in the treatment of hepatocarcinoma (HCC): effect on cell proliferation in HCC cell lines

Pivonello Claudia , Provvisiero Donatella Paola , Negri Mariarosaria , Di Gennaro Gilda , De Angelis Cristina , Simeoli Chiara , Patalano Roberta , De Martino Maria Cristina , Colao Annamaria , Pivonello Rosario

HCC is one of the most common malignancies worldwide. Local approaches are generally preferred for patients whose disease is restricted to the liver. In patients with extrahepatic disease systemic therapy can be considered. Chemotherapy did not demonstrate convincing survival advantages in several trails for HCC patients. Presently, the kinase inhibitor sorafenib is the only approved systemic target therapy for the treatment of advanced HCC. Mitotane (dichlorodiphenildichloroe...

ea0041ep712 | Male Reproduction | ECE2016

The role of dopamine pathway on human sperm: in vitro effect of dopamine receptor agonists and antagonists on sperm motility, kinetics and viability

de Angelis Cristina , Cariati Federica , Galdiero Giacomo , Coppola Giorgio , Galdiero Mariano , Salzano Ciro , Pivonello Claudia , Patalano Roberta , Alviggi Carlo , De Placido Giuseppe , Colao Annamaria , Pivonello Rosario

Dopamine has been found in both semen and oviductal fluid and dopamine receptors (DRs) have been detected in male genital tract and spermatozoa, suggesting that dopamine system may control important reproductive functions in humans. Dopamine improves sperm motility and viability in animal models, although the underlying molecular mechanisms have not been fully elucidated. The aims of this study were to investigate DRs expression in human spermatozoa and to evaluate the in ...

ea0070aep341 | Diabetes, Obesity, Metabolism and Nutrition | ECE2020

Anti-Lipogenic effects of cabergoline: A molecular study on white adipocytes

Negri Mariarosaria , Pivonello Claudia , Amatrudo Feliciana , Gentile Annalisa , Montò Tatiana , Patalano Roberta , Simeoli Chiara , Ferrigno Rosario , Simona Auriemma Renata , Colao Annamaria , Pivonello Rosario

Obesity is caused by an imbalance between dietary energy intake and expenditure. The expression of dopamine receptors (DRs) in human adipocytes has been already demonstrated. Moreover, the effects of cabergoline, a dopamine agonist, on body weight and glucose homeostasis in obese subjects without hyperprolactinemia has already been reported. The current study investigates the effects of cabergoline on lipid accumulation and on adipogenic factors expression to better define the...

ea0070aep530 | Endocrine-related Cancer | ECE2020

Reversal effect of Vitamin D (VitD) on exosome-mediated everolimus resistance (EveR) in hepatocellular carcinoma (HCC) cell line: A molecular study

Gentile Annalisa , Negri Mariarosaria , Montò Tatiana , Patalano Roberta , de Angelis Cristina , Simeoli Chiara , Ferrigno Rosario , Cristina De Martino Maria , Simona Auriemma Renata , Colao Annamaria , Pivonello Rosario , Pivonello Claudia

Cancer exosomes emerged as important mediators of cancer drug resistance. Drug exposure-mediated EveR in HCC cell lines can be reversed by VitD pre- and co-treatment. The current study aimed to investigate whether cancer exosomes from EveR HCC cell line are able to induce EveR in parental cells and whether VitD may activate reversal effect on exosome-mediated EveR.To this purpose, parental JHH-6 and EveR JHH-6 cell line were used. The uptakeof exosomes, ...

ea0056p674 | Nuclear receptors and Signal transduction | ECE2018

1,25 dihydroxyvitamin D reverses everolimus resistance in hepatocellular carcinoma activating mesenchymal-epithelial transition and miR-375

Pivonello Claudia , Paola Provvisiero Donatella , Negri Mariarosaria , Di Gennaro Gilda , de Angelis Cristina , Patalano Roberta , Galdiero Giacomo , Simeoli Chiara , Simona Auriemma Renata , Cristina De Martino Maria , Colao Annamaria , Pivonello Rosario

HCC is a difficult-to-treat-cancer with poor prognosis. EVOLVE-1 trial demonstrated that EVE did not improve overall survival in molecularly and clinically unselected patients with advanced HCC resistant to sorafenib. In selected patients, the well-established antitumor effect of EVE could make this a potential adjuvant therapy. Unfortunately, EVE acquired resistance due to the tumor adaptation to chronic drug use is a current challenge. VitD was deemed as potential regimen to...

ea0073yi1 | Young Investigator Awards | ECE2021

Circulating myomiRNAs as biomarkers of Cushing’s syndrome

Pivonello Claudia , Patalano Roberta , Negri Mariarosaria , Montò Tatiana , Amatrudo Feliciana , Paola Nicola Di , Iacono Marina , Simeoli Chiara , Ferrigno Rosario , Solari Domenico , Angelis Cristina De , Auriemma Renata Simona , Cappabianca Paolo , Colao Annamaria , Pivonello Rosario

Cushing’s syndrome (CS) is a rare endocrine disease caused by a chronic exposure to endogenous cortisol or exogenous glucocorticoids (GCs). Among multiple comorbidities, impairment of skeletal muscle mass and strength affects 40–70% of patients with active CS and persist even after long-term disease remission. In pathological conditions, GCs excess sustain muscle atrophy and weakness affecting type II muscle fibers. Muscle-specific microRNAs, defined myomiRs, are in...