Searchable abstracts of presentations at key conferences in endocrinology

ea0081rc1.6 | Rapid Communications 1: Diabetes, Obesity, Metabolism and Nutrition 1 | ECE2022

Evidence for three superimposed components, via which metformin affects blood glucose in obese mice

Kaplanian Mairam , Philippe Cecile , Beghini Marianna , Metz Matthaeus , Abu Eid Sameer , Kautzky-Willer Alexandra , Scherer Thomas , Furnsinn Clemens

Introduction: Despite a plethora of suggested targets and pathways, the mechanism of anti-hyperglycaemic metformin action is still unknown. The present study critically re-analysed protocols broadly applied in preclinical rodent studies.Methods: Obese male C57BL/6J mice on high fat diet were treated with metformin in the form of a single dose, daily intraperitoneal injections, admixture to drinking water, or continuous infusion via intraperitoneal minipu...

ea0081ep362 | Diabetes, Obesity, Metabolism and Nutrition | ECE2022

Imidazole-osmium reduces elution of lipids from cryofixed rat hepatic tissue for ultrastructural analysis

Durr Sabine , Reipert Siegfried , Krššak Martin , Philippe Cecile , Ehret Viktoria , Luft Matthias , Schintlmeister Arno , Scherer Thomas , Furnsinn Clemens

Introduction: Transmission electron microscopy (TEM) is the main tool for studying the ultrastructural properties of metabolically diseased tissue. For impactful micrographs as well as for interpretation of TEM specimens, the integrity of cellular components is crucial. Elution of substances is a major problem in TEM preparation with no universal solution found so far. Investigating fatty liver disease in a rat model, an important factor is the depiction of undamaged lipid dro...