Searchable abstracts of presentations at key conferences in endocrinology

ea0035p1126 | Thyroid Cancer | ECE2014

TERT promoter mutations correlate with a more advanced stage at diagnosis and with a poorer prognosis in differentiated thyroid cancer

Colombo Carla , Muzza Marina , Rossi Stefania , Cirello Valentina , Perrino Michela , Tosi Delfina , Vigo Beatrice , Leo Simone De , Bulfamante Gaetano , Magnani Elisa , Pignatti Elisa , Simoni Manuela , Vicentini Leonardo , Fugazzola Laura

Telomerase is a ribonucleoprotein polymerase that maintains telomere ends and plays a role in cellular senescence, being repressed in postnatal somatic cells. Mutations C228T and C250T of the telomerase reverse transcriptase (TERT) were recently reported in human cancers. In thyroid cancer, TERT mutations are more frequent in aggressive histotypes, but very few data are available about the potential correlations with clinical features in papillary and follicular thyroid cancer...

ea0037ep168 | Reproduction, endocrine disruptors and signalling | ECE2015

Heterogeneity of human chorionic gonadotropin (hCG) in commercial preparations of hCG and human menopausal gonadotropin

Riccetti Laura , Pignatti Elisa , Simoni Manuela

Introduction: Human chorionic gonadotropin (hCG) is a glycoprotein hormone composed by two subunits, an alpha subunit common to all gonadotropins and a specific beta subunit. hCG is produced by trophoblast cells as differently glycosylated isoforms with a wide range of molecular weight, from 30 to 50 KDa (‘regular’ hCG is 37 KDa). hCG is used in the treatment of infertility. The aim of this study is to analyse and compare the composition of different commercial hCG.<...

ea0026p124 | Female reproduction | ECE2011

Two hormone for one receptors: dissecting out LH and hCG activity with an in vitro approach.

Casarini Livio , La Marca Antonio , Pignatti Elisa , Simoni Manuela

Introduction: LH and hCG act on the same receptor (LHCGR), have different half-lives and in vivo biopotency. It is not known whether they elicit the same cellular and molecular response. The aim of this study was to compare the kinetics of cAMP response to recombinant LH and hCG.Design: In COS-7 cells permanently expressing the human LHCGR (COS-7/LHCGR) we evaluated LH and hCG dose-response curves, by measuring total cAMP after 3 h of incubation. ...

ea0022p742 | Steroid metabolism &amp; action | ECE2010

Tissue expression of the CYP19A1 gene and aromatase protein in male cynomolgus monkeys (Macaca fascicularis)

Pignatti Elisa , Lachhab Asmaa , Casarini Livio , Wistuba Joachim , Schlatt Stefan , Carani Cesare , Simoni Manuela

Objective: Aromatase, the key enzyme involved in estrogen synthesis, is expressed in a variety of cells, including peripheral blood leukocytes (PBLs), and tissues. The systematic study of aromatase expression and activity in different tissues in the human and other primates, however, is difficult and still missing. Although is not really known, aromatase expression in PBLs could be used as parameter of aromatase expression/activity making PBLs a potential alternative for other...

ea0020p483 | Obesity and Metabolism | ECE2009

Aromatase expression in peripheral blood leukocytes from adult and elderly female and male subjects

Pignatti Elisa , Rossi Alessandra , Scaltriti Sara , Taliani Erica , Rochira Vincenzo , Simoni Manuela , Carani Cesare

Objective: Aromatase, the key enzyme involved in estrogen synthesis, is expressed in a variety of cells and tissues including human peripheral blood leukocytes (PBLs). The present study was designed to evaluate PBL aromatase gene expression in male and female elderly subjects compared to young male controls.Design: CYP19A1 mRNA and protein were measured in PBLs obtained from young men (n=13) aged < 35 years, postmenopausal women (n</...

ea0037oc4.3 | Diabetes | ECE2015

Chronic, long-term administration of Vardenafil improves endothelial function and corrects hypogonadism in patients with type 2 diabetes mellitus. A longitudinal, prospective, randomized, placebo-controlled, double blind, clinical trial

Santi Daniele , Guidi Alessandro , Granata Antonio , Pignatti Elisa , Bozic Roberto , Zaza Stefano , Roli Laura , Trenti Tommaso , Carani Cesare , Simoni Manuela

Background: Endothelial dysfunction leads to cardiovascular complications in type 2 diabetes mellitus (T2DM), through a reduction of nitric oxide (NO)-mediated relaxation. Phosphodiesterase-5 inhibitors (PDE5i) have hemodynamic effects, improving NO levels.Aim: To investigate if long term, chronic treatment with the PDE5i Vardenafil improves systemic endothelial function in men with T2DM.Methods: A longitudinal, prospective, invest...

ea0070aep880 | Thyroid | ECE2020

Clinical practice survey on BRAF V600E role in the therapeutic decision in indeterminate thyroid cytology

Brigante Giulia , Craparo Andrea , Pignatti Elisa , Marino Marco , Casarini Livio , Sperduti Samantha , Boselli Gisella , Margiotta Gianluca , Rochira Vincenzo , Simoni Manuela

Introduction: The use of multigene panels in thyroid nodule diagnosis is still limited, due to high costs and need for ad hoc sampling. Since BRAF-V600E is the commonest genetic alteration in differentiated thyroid cancer, this is the mostly tested genetic parameter in clinical practice.Aim: To evaluate the use of BRAF mutation analysis in wash-out liquid from fine needle aspiration (FNA) in clinical practice, characterizing th...

ea0032p640 | Male reproduction | ECE2013

Complete aromatase deficiency in four adult men: detection of a novel mutation and two known mutations in the CYP19A1 gene

Pignatti Elisa , Unluhizarci Kursad , Kartal Ermine , Ajlouni Kamel , Khawaja Nahla , Carani Cesare , Marino Marco , Simoni Manuela , Vighi Eleonora , Rochira Vincenzo

Introduction: At present, only eight men with loss-of-function mutations in the CYP19A1 gene have been described. Here we report the genetic study of four adult men with undetectable serum estrogens, unfused epiphyses, eunuchoid skeletal proportions, continuing linear growth, tall stature, genu valgum, osteoporosis, obesity and achantosis nigricans. Patient 1 (26-years/182 cm) and 2 (28-years/187 cm) are from Turkey with a history of consanguinity. Patient 3 (44-years...

ea0032p1088 | Thyroid cancer | ECE2013

Pre-miR146a expression profiling of follicular thyroid carcinoma

Pignatti Elisa , Vighi Eleonora , Roncati Luca , Kara Elda , Porcheddu Eleonora , Magnani Elisa , Rochira Vincenzo , Maiorana Antonino , Carani Cesare , Simoni Manuela

Introduction: Follicular thyroid carcinomas (FTC) are the second most common type of thyroid malignancy. Micro-RNAs (miRNAs) are a new class of small, noncoding RNAs whose expression is deregulated in many types of human cancers and may lend novel clues to FTC genesis. Pre-miR146a represents one of the most up-regulated miRNAs in papillary thyroid carcinomas (PTC) and a SNP (rs2910164), identified in pre-miR146a, contributes to genetic predisposition to PTC, but data on FTC ar...

ea0032p1092 | Thyroid cancer | ECE2013

Mutations and somatic changes in the genotype of rs2910164 in pre-miR146a are frequent in follicular thyroid carcinoma

Vighi Eleonora , Pignatti Elisa , Roncati Luca , Rochira Vincenzo , Kara Elda , Madeo Bruno , Magnani Elisa , Maiorana Antonino , Carani Cesare , Simoni Manuela

Introduction: The mechanism underlying the development of follicular thyroid carcinoma (FTC), the second commonest thyroid tumor, is still unknown. An interesting hypothesis is that changes in the expression of multiple regulatory RNA (miR) genes may be a major mechanism in thyroid carcinogenesis. For example, several studies suggested that SNP rs2910164 in the pre-miR146a, a precursor of miR146a, might be correlated to papillary carcinoma. The aim of this study was the evalua...