Searchable abstracts of presentations at key conferences in endocrinology

ea0014p376 | (1) | ECE2007

Increase of L-thyroxine requirement during pregnancy

Russo Laura , Tonacchera Massimo , Gianetti Elena , Vitti Paolo , Pinchera Aldo

In pregnant women with a thyroid disease an increased amount of LT4 may be required for the correction of hypothyroidism or treatment of nodular goiter.Aim of this study was to assess the amount of the variations of LT4 requirement in pregnant women with thyroid diseases.To address this issue, we retrospectively evaluated a cohort of 138 women treated with LT4 divided in two groups: 47 euthyroid (E) (affected by nodular goiter (NG)...

ea0014p377 | (1) | ECE2007

Safety of pharmacological treatment of thyroid diseases &br;during pregnancy

Gianetti Elena , Tonacchera Massimo , Russo Laura , Vitti Paolo , Pinchera Aldo

Pregnant women may require treatment of hypo- or hyperthyroidism, L-thyroxine (LT4), propylthiouralcil (PTU) and methimazole (MMI) being the most frequently used drugs. Aim of this study was to verify the consequences of pharmacological treatment during pregnancy. We retrospectively evaluated 379 pregnancies: 124 patients under MMI treatment, 35 of whom still hyperthyroid in spite of treatment (H-MMI) and 89 euthyroid (E-MMI); 52 GD patients under PTU, 20 of whom still hyperth...

ea0022p103 | Bone/Calcium | ECE2010

Tumoral calcinosis likely due to end-organ resistance to FGF23

Cianferotti Luisella , Vignali Edda , Cetani Filomena , Galli Giulia , Giacomelli Tamara , Pinchera Aldo , Marcocci Claudio

Tumoral calcinosis is a rare autosomal recessive disease characterized by hyperphosphatemia due to increased renal phosphate reabsorption leading to soft tissue calcifications. The levels of fibroblast growth factor 23 (FGF23), a hormone required for normal renal phosphate reabsorption, are typically low in the classic form of the disease due to homozygous missense mutations in FGF23 or in the UDP-Nacetyl-alpha-D galactosamine:polypeptide N-acetylgalactos...

ea0020oc1.1 | Endocrine Tumours | ECE2009

Optimizing time of prophylactic surgery in ret gene carriers on the basis of serum calcitonin

Elisei Rossella , Romei Cristina , Bottici Valeria , Cosci Barbara , Renzini Guilia , Molinaro Eleonora , Agate Laura , Pinchera Aldo

Multiple endocrine neoplasia type 2 (MEN 2) is characterized by the presence of medullary thyroid cancer (MTC) and other benign pathologies. RET mutations are responsible of this disease and their screening is a very sensitive tool for the identification of gene carriers (GC).Aim of this study was to verify the relevance of the basal and pentagastrin (Pg) stimulated serum calcitonin (CT) in the decision to perform TT in GC. We reviewed data of 65 GC foun...

ea0014oc5.2 | Thyroid basic | ECE2007

Tyroglobulin (Tg) depletion in receptor associated protein (RAP) KO mice is due to a reduction of Tg aggregates

Lisi Simonetta , Botta Roberta , Pinchera Aldo , Marcocci Claudio , Marinò Michele

RAP KO mice have a reduction of colloidal Tg resulting in subclinical hypothyroidism and histological signs of goiter. The difference in colloidal Tg between RAP KO and WT mice was striking by immunohistochemistry, but could not be detected in thyroid extracts. To explain this discrepancy, we hypothesized that the reduction of Tg reflected a reduction of Tg aggregates discarded during tissue extraction. To investigate this possibility, pellets obtained by thyroid homogenizatio...

ea0014p364 | (1) | ECE2007

Comparison between serum calcitonin (CT) levels following Pentagastrin (Pg) and Calcium (CA) stimulus

Bottici Valeria , Sculli Mariangela , Renzini Giulia , Passannanti Paolo , Lucchetti Fabiana , Grasso Lucia , Pinchera Aldo , Elisei Rossella

Serum CT is the most specific marker of Medullary Thyroid carcinoma (MTC). Although Pg test is the most frequently used to induce CT secretion, the poor availability of Pg makes it necessary to look for different agents. Aim of this work was to compare the induction of CT secretion following 2 different stimuli in the same patient. We studied 25 patients (14 females, 11 males; mean age 50±15 yrs; range 12–77 yrs). All patients were subjected to both tests by injectio...

ea0014p399 | (1) | ECE2007

Prevalence of primary hyperparatiroidism in treated and untreated breast cancer

Giani Claudio , Fiore Emilio , Giustarini Elisa , Muller Ilaria , Vignali Edda , Fierabracci Paola , Pinchera Aldo

Hypercalcemia is a frequent metabolic disorder in metastatic breast cancer (BC). Aim of this study was to evaluate the incidence of hypercalcemia due to PHPT in BC patients. The study group included 271 consecutive BC, mean age±S.D. 57.7±11.96 yrs. 100/271(36.9%) evaluated at different times after mastectomy (A) and 171(63.1%) before surgery (B), with no distant metastases. Age matched control group included 108 healthy women (Co) and 70 women with thy...

ea0014oc5.4 | Thyroid basic | ECE2007

BRAFV600E mutations but not RET/PTC rearrangements are correlated with a lower expression of NIS mRNA expression in papillary thyroid cancer (PTC)

Romei Cristina , Piampiani Pamela , Faviana Pinuccia , Basolo Fulvio , Bottici Valeria , Sculli Mariangela , Berti Piero , Materazzi Gabriele , Pinchera Aldo , Elisei Rossella

Several studies have identified a relationship between oncogene activation and dedifferentiation of PTC. Mutations of RAS, RET/PTC and BRAF modulate the expression of thyroid genes. An impaired NIS expression has been demonstrated in PTCs harboring the BRAFV600E mutation.Aim of this study was to analyze BRAF and RET/PTC1-3 alterations and their influence on the expression of thyroid differentiation genes. Seventy-one PTC samples were studied. Quantitative analysis o...

ea0014p103 | (1) | ECE2007

Prognostic value of anti-thyroperoxidase antibodies in high malignancy degree breast cancer

Fiore Emilio , Giani Claudio , Giustarini Elisa , Muller Ilaria , Mammoli Claudia , Campani Daniela , Roncella Manuela , Fustaino Loredana , Pinchera Aldo

A high incidence of serum anti thyro-peroxidase antibodies (TPOAb) has been found in breast cancer (BC). Aim of this study was to evaluate the predictive value of TPOAb in BC. The study group included 47 women submitted to mastectomy for high malignancy degree BC (tumor size >5 cm and/or n° lymph-nodes >3), followed for 5 years. No patient had distant metastases. All were evaluated for thyroid disorders after breast surgery and before any anti-tumoral adjuvant the...

ea0014p345 | (1) | ECE2007

Characterization of facilitative glucose transporters (GLUT) in human thyroid carcinoma cell lines

Ciampi Raffaele , Vivaldi Agnese , Piampiani Pamela , Agate Laura , Salvadori Piero , Del Guerra Alberto , Pinchera Aldo , Elisei Rossella

18FDG-PET is based on the capability of tumor cells to take up glucose. An increment in expression of the glucose transporter 1 (GLUT1) has been observed in thyroid tumors with poor prognosis but very few data are available about the expression of other glucose transporters in thyroid. Here, we study the expression and function of GLUT isoforms 1, 2, 3, 4, 6, 8, 10, 12 in human thyroid carcinoma cell lines ARO and FRO (anaplastic carcinoma), NPA (poorly-differentiated papillar...