Searchable abstracts of presentations at key conferences in endocrinology

ea0041gp127 | Neuroendocrinology | ECE2016

Hypothalamic GRK2, via GPR54, modulates puberty onset

Avendano Ma Soledad , Ruiz-Pino Francisco , Vazquez Ma Jesus , Velasco Inmaculada , Heras Violeta , Torres Encarni , Manuel Castellano Juan , Roa Juan , Pinilla Leonor , Tena-Sempere Manuel

The G protein-coupled receptor (GPCR) kinase 2, GRK2, is a ubiquitous serine/threonine protein kinase that is able to phosphorylate and desensitize the active form of several GPCR. Compelling, as yet limited, evidence from in vitro studies have suggested a potential role of GRK2 in mediating desensitization of Gpr54, the canonical receptor for kisspeptins that is abundantly expressed in GnRH neurons. Yet, although kisspeptins have been universally recognized as essent...

ea0041ep789 | Obesity | ECE2016

Intergenerational influence of paternal obesity on metabolic and reproductive health of the offspring: male-preferential impact and potential involvement of Kiss1-mediated pathways

Sanchez-Garrido Miguel Angel , Ruiz-Pino Francisco , Barroso Alexia , Velasco Inmaculada , Heras Violeta , Jesus Vazquez Maria , Castellano Juan Manuel , Roa Juan , Pinilla Leonor , Tena-Sempere Manuel

Obesity and its comorbidities are reaching epidemic proportions. Maternal obesity is known to predispose the offspring to metabolic disorders, independently of genetic inheritance. This intergenerational transmission has also been suggested for paternal obesity during the pre-conception stage, as it appears to have a negative impact on the metabolic and reproductive health of the offspring, likely via epigenetic changes in spermatozoa. However, whether paternal obesity sensiti...

ea0032p684 | Neuroendocrinology | ECE2013

Roles of kisspeptin partners, NKB and dynorphin, in the control of gonadotropin secretion: revisiting the KNDy paradigm

Ruiz-Pino Francisco , Garcia-Galiano David , Manfredi-Lozano Maria , Leon Silvia , Sanchez-Garrido Miguel A , Roa Juan , Pinilla Leonor , Navarro Victor , Tena-Sempere Manuel

KNDy neurons, which co-express kisspeptins, neurokinin B (NKB) and dynorphin (Dyn), play a key role in the tonic control of gonadotropin secretion by modulating the GnRH release. The assumed KNDy model proposes that NKB and Dyn (as stimulatory and inhibitory signals, respectively) auto-regulate the secretion of kisspeptins onto GnRH neurons. However, some aspects of this paradigm remain poorly defined. In this context, the aims of this work were to characterize i) the putative...

ea0022p592 | Neuroendocrinology and Pituitary (<emphasis role="italic">Generously supported by Novartis</emphasis>) | ECE2010

The anorexigenic neuropeptide, NUCB2/nesfatin-1, is indispensable for normal puberty onset in the female rat

Garcia-Galiano David , Navarro Victor M , Roa Juan , Ruiz-Pino Francisco , Castellano Juan M , Dieguez Carlos , Pinilla Leonor , Tena-Sempere Manuel

The hypothalamic peptide, nesfatin-1, derived from the precursor NEFA/nucleobindin2 (NUCB2), was recently identified as central anorexigenic molecule, acting in a leptin-independent manner. Yet, its potential involvement in the regulation of other biological functions gated by body energy status remains unexplored. We show herein that NUCB2/nesfatin-1 is involved in the control of female puberty. NUCB2/nesfatin mRNA and protein were detected at the hypothalamus of pubertal fem...

ea0022p654 | Neuroendocrinology and Pituitary (<emphasis role="italic">Generously supported by Novartis</emphasis>) | ECE2010

Presence of kiss1/kiss1r system at the pituitary, testis and adipose tissue in rodents: functional actions and regulation by physiological cues

Gutierrez-Pascual Ester , Cordoba-Chacon Jose , Pinilla Leonor , Gracia-Navarro Francisco , Luque Raul M , Malagon Maria M , Tena-Sempere Manuel , Martinez-Fuentes Antonio J , Castano Justo P

It is now widely accepted that the kisspeptins (kps) and their receptor kiss1r play an essential role in the neuroendocrine regulation of the reproductive axis by stimulating hypothalamic GnRH secretion. However, kiss1 and kiss1r expression in tissues distinct from, but related to, the hypothalamus, prompted us to propose that these molecules may exert regulatory functions in additional places of the neuroendocrine system, such as the pituitary and two of its phy...

ea0063oc8.4 | Reproduction 1 | ECE2019

Conditional ablation of AMP-Activated Protein Kinase (AMPK) in GnRH neurons reveals specific roles in reproductive and metabolic homeostasis

Roa Juan , Franssen Delphine , Barroso Alexia , Ruiz-Pino Francisco , Vazquez Maria Jesus , Garcia-Galiano David , Castellano Juan Manuel , Gaytan Francisco , Dieguez Carlos , Pinilla Leonor , Lopez Miguel , Tena-Sempere Manuel

Reproduction is tightly coupled to body energy and metabolic status. GnRH neurons, the final output pathway for the brain control of reproduction, directly or indirectly receive and integrate multiple signals, including metabolic cues regulating reproductive function. Yet, the molecular underpinnings of such phenomenon remain largely unfolded. AMP-activated protein kinase (AMPK), a fundamental cellular sensor that becomes activated in conditions of energy deficit, has a key ro...

ea0037oc7.4 | Neuroendocrinology and pituitary-basic | ECE2015

Gonadotropin-inhibitory hormone signalling displays sexually dimorphic roles in the control of energy homeostasis: studies in the NPFF1 receptor null mouse

Leon Silvia , Vazquez Maria Jesus , Barroso Alexia , Garcia-Galiano David , Ruiz-Pino Francisco , Manfredi-Lozano Maria , Romero-Ruiz Antonio , Sanchez-Garrido Miguel A , Dieguez Carlos , Nogueiras Ruben , Roa Juan , Pinilla Leonor , Tena-Sempere Manuel

RF-amide-related peptide-3 (RFRP-3), the mammalian orthologue of the avian gonadotropin-inhibiting hormone (GnIH), has been proposed as major inhibitory signal for the reproductive axis, acting via the NPFF1 receptor (NPFF1R). In addition, RFRP-3 has been recently suggested to modulate feeding, with reported orexigenic actions; a function that might contribute to the integrative control of energy homeostasis and reproduction. However, characterisation of the metabolic effects ...

ea0037gp.13.04 | Diabetes and obesity – Translational cardiovascular and obesity | ECE2015

Metabolic and gonadotropic impact of sequential obesogenic insults in the female: influence of the loss of ovarian secretion

Sanchez-Garrido Miguel A , Ruiz-Pino Francisco , Manfredi-Lozano Maria , Leon Silvia , Heras Violeta , Castellano Juan M , Vazquez Maria Jesus , Roa Juan , Romero-Ruiz Antonio , Dieguez Carlos , Pinilla Leonor , Tena-Sempere Manuel

While female fertility is known to be sensitive to conditions of low body fuel reserves, the reproductive consequences of persistent energy excess remain ill defined. Yet, the pandemic proportions of obesity, and its potential impact on the gonadotropic axis, call for a better understanding of this phenomenon. Alike, the influence of ovarian hormones on the pathophysiology of obesity and its complications merits further investigation. In this work, we aimed to elucidate the me...

ea0056oc2.4 | Look who is controlling your gonads! | ECE2018

Novel role of central ceramide signaling in mediating obesity-induced precocious puberty

Heras Violeta , Castellano Juan Manuel , Fernandois Daniela , Velasco Inmaculada , Roa Juan , Vazquez Maria J. , Ruiz-Pino Francisco , Pineda Rafael , Torres Encarnacion , Avendano Maria Soledad , Gaytan Francisco , Pinilla Leonor , Lopez Miguel , Casals Nuria , Tena-Sempere Manuel

Childhood obesity has become a major health problem, which is coupled to different adverse outcomes and diseases. The escalating prevalence of child obesity parallels that of alterations in pubertal timing, also linked to higher disease burden later on life. Yet, the mechanisms underlying for this association remain unfolded. Ceramides, ubiquitous signaling molecules involved in numerous biological processes, have emerged as mediators of metabolic disorders and transmitters fo...

ea0056gp141 | Neuroendocrinology | ECE2018

Reproductive and metabolic consequences of AMP-Activated Protein Kinase (AMPK) ablation in GnRH neurons

Barroso Alexia , Franssen Delphine , Ruiz-Pino Francisco , Vazquez Maria Jesus , Garcia-Galiano David , Castellano Juan Manuel , Onieva Rocio , Rodriguez Ana Belen , Gaytan Francisco , Dieguez Carlos , Pinilla Leonor , Lopez Miguel , Roa Juan , Tena-Sempere Manuel

GnRH neurons are the final output of the brain controlling reproduction. These neurons receive information from multiple central and peripheral signals, ranging from gonadal steroids to metabolic cues and brain neuropeptides, to regulate their activity. AMP-activated protein kinase (AMPK) is an intracellular sensor, activated by energy deficiency, involved in the regulation of the cellular and whole-body energy homeostasis. Previous in vitro studies suggested the part...