Searchable abstracts of presentations at key conferences in endocrinology

ea0014p117 | (1) | ECE2007

A novel activating germline mutation in the RET gene (Y606C) in a patient with medullary thyroid carcinoma

Luconi Michaela , Ercolino Tonino , Lombardi Adriana , Becherini Lucia , Piscitelli Elisabetta , Sole Gaglianò Maria , Serio Mario , Mannelli Massimo

Germline mutations in the RET gene cause MEN2, an inherited cancer syndrome associated with medullary thyroid carcinoma (MTC). We performed genetic analysis on DNA from whole blood of a 58 yr old female affected by a multifocal MTC. Exons 10, 11, 13, 15 and 16 of RET gene were amplified by PCR using specific primers and characterised by direct automatic sequencing. Here, we report a new RET point mutation: a heterozigous missense mutation Y606C, a G to A nucleotide substitutio...

ea0014p13 | (1) | ECE2007

Rosiglitazone interferes with the inflammatory response induced in human endothelial cells by TNFalpha and IFNgamma through a new mechanism involving extracellular signal-regulated kinases (ERKs)

Lombardi Adriana , Piscitelli Elisabetta , Francalanci Michela , Mello Tommaso , Santarlasci Veronica , Gelmini Stefania , Cantini Giulia , Galli Andrea , Cosmi Lorenzo , Annunziato Francesco , Forti Gianni , Serio Mario , Luconi Michaela

Microvascular endothelium is one of the main character and target involved in the inflammatory response. Upon specific activation, endothelial cells massively recruit Th1 IFNgamma secreting lymphocytes at the inflammatory site. In the present study, we investigate the intracellular signalling mediating TNFalpha (T) and IFNgamma (I) inflammatory response in a human endothelial cell line (HMEC-1) in vitro and the interfering effects of rosiglitazone (RGZ), a peroxisome pr...