Searchable abstracts of presentations at key conferences in endocrinology

ea0042il9 | Metabolism in Prostate Cancer | Androgens2016

Regulating androgen action by steroid synthesis and metabolism

Poutanen Matti

Recent results from us and others have shown that concentrations of sex steroids in serum/circulation do not always reflect their intra-tissue levels in hormone-dependent tissues. This proposes that sex steroid metabolism plays a key role in determining the final concentration of active sex steroids in their target tissues. According to the current knowledge, members of three enzyme families, namely cytochrome P450 (CYP), aldo-keto reductase (AKR) and short-chain dehydrogenase...

ea0037s9.1 | Steroid hormone action in target tissues | ECE2015

Tissue-specific sex steroid action: role of HSD17B-enzymes

Poutanen Matti

Recent data from us and others have evidently shown that steroid metabolism at the target tissues is a key determinant regulating the availability of high affinity ligands for steroid receptors. Thus, the serum concentrations of the steroids do not necessary mimic their target tissue concentrations in several physiological and pathophysiological settings. Accordingly, among the other steroid metabolizing enzymes, the expression of hydroxysteroid dehydrogenase- (HSD17B) enzymes...

ea0026mte23 | (1) | ECE2011

Transgenic animals in endocrinology

Poutanen Matti

Mice have been used as a model system in biomedicine for several decades. As mammals their relevance to human endocrinology is substantially greater than many other model systems such as flies, worms and yeast. Mice are also easily adapted to various experimental conditions with human relevance. Most importantly, the mouse genome is well annotated, it can be precisely engineered by several powerful ways, and international consortia have recently produced variety novel tools fo...

ea0042p40 | (1) | Androgens2016

Segment-specific enrichment of AP-2 and Runx motifs within caput- and IS-preferred androgen receptor binding sites in the mouse epididymis

Pihlajamaa Paivi , Sahu Biseajyoti , Janne Olli A. , Poutanen Matti , Sipila Petra

Epididymis is an androgen dependent organ with four main segments; initial segment (IS), caput, corpus and cauda. It is an essential site for post-testicular sperm maturation, as spermatozoa acquire fertilizing capacity during epididymal transit. Post-testicular maturation is dependent on the proteins expressed and secreted to the lumen by epididymal cells. Androgens regulate gene expression in epididymis in a segment-specific manner. Caput-specific genes are androgen regulate...

ea0049oc3.4 | Receptors & Signalling | ECE2017

Treatment with thyroid hormone metabolite 3,5-T2 alters cholesterol and sex steroid metabolism in mouse liver

Lietzow Julika , Golchert Janine , Homuth Georg , Jonas Wenke , Ohlsson Claes , Landin Andreas , Poutanen Matti , Kohrle Josef

Recent findings revealed 3,5-diiodo-L-thyronine (3,5-T2) as a metabolically active iodothyronine affecting hepatic energy and lipid metabolism. Therefore, 3,5-T2 has been proposed as a potential hypolipidemic agent for treatment of obesity and its hepatic aftermath. So far only limited data exists relating to the effects of 3,5-T2 on metabolism in euthyroid mammals maintained on high-fat diet from the day of weaning onwards. Therefore, we performed a study in wh...

ea0049oc13.3 | Reproduction & Endocrine Disruption | ECE2017

Sertoli cell expressed hydroxysteroid (17beta) dehydrogenase 1 is required for male fertility

Hakkarainen Janne , Jokela Heli , Zhang Fuping , Kotaja Noora , Sipila Petra , Poutanen Matti

: Hydroxysteroid (17beta) dehydrogenase 1 (Hsd17b1) is a steroidogenic enzyme catalyzing the conversion of estrone (E1) to estradiol (E2), and androstenedione (A-dione) to testosterone (T). We have shown that the deletion of Hsd17b1 gene in mice resulted in the failure of ovarian estrogen production and subfertility. In this study, we clarified the role of Hsd17b1 in male reproduction. The data revealed that in mice Hsd17b1 mRNA is highly expressed in the Sertoli cells at the ...

ea0049ep364 | Cardiovascular Endocrinology and Lipid Metabolism | ECE2017

Deleting genomic region of Hsd17b1 in mice results into a hypomorphic Naglu allele, and consequently to a phenotype mimicking a lysosomal storage disease

Jokela Heli , Hakkarainen Janne , Pakarinen Pirjo , Ruohonen Suvi , Zhang Fuping , Poutanen Matti

Hydroxysteroid 17-beta dehydrogenase 1 (HSD17B1) is an enzyme catalyzing the reduction of estrone (E1) to estradiol (E2), as well as androstenedione (Adione) to testosterone (T). To elucidate the physiological function of HSD17B1, we generated knockout mice with disrupted Hsd17b1 gene using targeted ES cells (clone 10231) obtained from KOMP repository ( In these ES cells the whole coding region of Hsd17b1 was replaced with LacZ/Neo cass...

ea0042oc17 | (1) | Androgens2016

miR-32 promotes replicative changes in prostate epithelium in vivo

Latonen Leena , Gillen Andrew , Valkonen Mira , Ruusuvuori Pekka , Zhang Fuping , Poutanen Matti , Visakorpi Tapio

The androgen receptor (AR) signaling pathway is central to the emergence of castration-resistant prostate cancer (CRPC). miR-32 is an androgen regulated miRNA which is differentially expressed in CRPC compared to benign prostatic hyperplasia (BPH) and able to provide a significant growth advantage to LNCaP cells. To study how increased miR-32 expression contributes to prostate cancer formation and/or progression in vivo, we have established transgenic mice expressing ...

ea0025p271 | Reproduction | SFEBES2011

Increased epididymal Rnase9 expression in Rnase10 (−/−) mice

Sharp Victoria , Krutskikh Anton , Dacheux Jean-Louis , Poutanen Matti , Huhtaniemi Ilpo

Spermatozoa are dependent on the proximal epididymis environment to complete their maturation. However, no single specific factor crucial for this process has been identified. Rnase9 and Rnase10 are expressed specifically in the murine proximal epididymis. Located on chromosome 14C1 only 28 kb apart, they do not show high homology with each other. An orthologue of both genes exist in the rat and human, and ‘Train A’ (porcine orthologue) is the most abun...

ea0022p485 | Female reproduction | ECE2010

The ovulatory capacity and related genes are altered in transgenic mice hypersecreting human chorionic gonadotrophin hormone (hCG)

Ratner Laura , Gonzalez Betina , Poutanen Matti , Huhtaniemi Ilpo , Calandra Ricardo , Rulli Susana

Hypersecretion of hCG in transgenic mice produces profound alterations in the ovary, thus triggering failures in ovulation and affecting fertility. The objective of this study was to analyze the response of immature female mice overexpressing moderated (hCGβ+; 0.1 IU/ml) and elevated (hCGαβ+; 7 IU/ml) levels of hCG to an ovulation induction protocol (PMSG 7.5 IU i.p.; after 48 h, hCG 7.5 IU i.p). Wild-type (WT) females subjected to the same protocol were used as...