Searchable abstracts of presentations at key conferences in endocrinology

ea0016oc6.1 | Metabolism and cardiovascular | ECE2008

The signaling profile of recombinant human orexin-2 receptor (OX2R)

Tang Jiyou , Chen Jing , Conner Alex , Punn Anu , Randeva Harpal

Orexin-A and orexin-B orchestrate their diverse central and peripheral effects via two G-protein coupled receptors, orexin-1 receptor (OX1R) and OX2R, which activate multiple G-proteins. In many tissues, orexins activate MAPKs, however, the mechanism by which OX2R alone mediates MAPK activation is not understood. We studied the intracellular signaling mechanisms involved in OX2R-mediated ERK1/2 and p38 MAPK activation. In HEK-293 cells stably over-expressing recombi...

ea0016p664 | Signal transduction | ECE2008

cAMP/PKA and ERK1/2 dependent feedback mechanisms regulating type 2 CRH receptor signalling

Markovic Danijela , Punn Anu , Lehnert Hendrik , Grammatopoulos Dimitris

Corticotropin-releasing hormone (CRH) and its related peptides, the urocortins (UCNs) mediate their effects by binding to two types of GPCRs, CRH-R1 and CRH-R2. In most target tissues, the adenylyl cyclase/cAMP/PKA and the mitogen activated protein kinase (MAPK) are the two main pathways mediating the biological effects of CRH-Rs. For most multi-signal G-protein coupled receptors (GPCRs), there is considerable level of ‘cross-talk’ between different signalling cascad...

ea0016p665 | Signal transduction | ECE2008

Signalling properties of corticotropin-releasing hormone receptor (CRH-R1/2) in adrenal (H295R) cells

Punn Anu , Ramanjaneya Manjunath , Lehnert Hendrik , Grammatopoulos Dimitris

Corticotropin-releasing hormone (CRH) and urocortins (UCN) bind to two types of GPCRs (CRH-R1 and CRH-R2), activating a plethora of signalling cascades, including the mitogen-activated protein kinases (MAPKs) pathways. Although previous studies have identified that CRH directly regulates adrenal steroidogenesis1, the second messenger systems and downstream effectors remain poorly defined. The adrenal cell line H295R, which express both CRH-Rs1 was used to...