Endocrine Abstracts

Orexin-A and orexin-B orchestrate their diverse central and peripheral effects via two G-protein coupled receptors, orexin-1 receptor (OX1R) and OX2R, which activate multiple G-proteins. In many tissues, orexins activate MAPKs, however, the mechanism by which OX2R alone mediates MAPK activation is not understood. We studied the intracellular signaling mechanisms involved in OX2R-mediated ERK1/2 and p38 MAPK activation. In HEK-293 cells stably over-expressing recombi...

Corticotropin-releasing hormone (CRH) and its related peptides, the urocortins (UCNs) mediate their effects by binding to two types of GPCRs, CRH-R1 and CRH-R2. In most target tissues, the adenylyl cyclase/cAMP/PKA and the mitogen activated protein kinase (MAPK) are the two main pathways mediating the biological effects of CRH-Rs. For most multi-signal G-protein coupled receptors (GPCRs), there is considerable level of ‘cross-talk’ between different signalling cascad...

Corticotropin-releasing hormone (CRH) and urocortins (UCN) bind to two types of GPCRs (CRH-R1 and CRH-R2), activating a plethora of signalling cascades, including the mitogen-activated protein kinases (MAPKs) pathways. Although previous studies have identified that CRH directly regulates adrenal steroidogenesis1, the second messenger systems and downstream effectors remain poorly defined. The adrenal cell line H295R, which express both CRH-Rs1 was used to...