Searchable abstracts of presentations at key conferences in endocrinology

ea0028p312 | Steroids | SFEBES2012

Effects of In-Utero Exposure to Endocrine Disruptors on the Ovine Fetal Adrenal Gland

Buckley Charlotte , Danga Mercy , Hogg Kirsten , Amezaga Maria , Rhind Stewart , Fowler Paul , Rae Michael , Morley Steven

Introduction: The repercussions of long-term exposure to low level mixtures of endocrine disrupting compounds (EDCs) which are present, ubiquitously, in the environment are not well understood. Heat-treated sewage sludge, an EDC-rich product used as fertiliser, has been shown to compromise testicular and ovarian development. However, to date, there has been limited investigation in exposed animals of the adrenal glands, the steroid hormone products of which play key roles in f...

ea0025p281 | Reproduction | SFEBES2011

HRPE773 (ZG16B) expression is elevated in human endometrium during the early secretory phase of the menstrual cycle and in uterine decidua following miscarriage

Ng Bonnie , McDonald Sarah , Ren Xia , Mullins John , Rae Michael , Critchley Hillary , Horne Andrew , Morley Steven

Introduction: Expression of murine CSP-1/Dcpp secretory proteins was first identified in sublingual salivary glands and subsequently in secretory epithelia of several other tissues, including the mouse female reproductive tract where expression is regulated by oestrogen. Preliminary studies indicated that HRPE773, the human CSP-1/Dcpp orthologue, displayed a similar pattern of expression to its murine counterparts. We therefore hypothesized that HRPE773 might be expressed in h...

ea0015oc13 | Reproduction | SFEBES2008

Effect of hypoxia and inflammatory and anti-inflammatory signals on gene expression in sheep ovarian surface epithelium in vitro

Harlow Christopher R , Rae Michael , Price Deborah , McNeilly Alan S , Rhind Stewart M , Hillier Stephen G

The intraperitoneal environment of the ovarian surface epithelium (OSE) has an oxygen (O2) tension of 5–12%. This could impact cellular events leading to ovulation. Furthermore, ovarian cancers arising from the OSE (∼80–90% of ovarian malignancies) are likely to experience increased hypoxia due to the relatively poor vascularisation of solid tumours.Using 1%, 5% or 20% O2, we measured the expression of HSD11B1, COX-2...

ea0059p038 | Adrenal and steroids | SFEBES2018

QRT-PCR analysis of the effect of in utero exposure to sewage sludge on steroidogenic gene expression in ovine foetal adrenal gland

Cooper Erin A , Reddy Sreedath , Allenson Abbie Z , Cooper Duncan P , Fowler Paul A , Rae Michael T , Morley Steven D

Endocrine disruptors are chemicals which in low concentrations can disturb gene expression in a range of endocrine glands and organs including the fetal and adult adrenal glands, potentially resulting in altered steroidogenic flux. With exposure to endocrine disruptors affecting both animals and humans, it is important to assess both the mechanisms and consequences of disruption in steroidogenic pathways, particularly as foetal development may be especially sensitive to endocr...

ea0015p185 | Endocrine tumours and neoplasia | SFEBES2008

Steroid Sulphatase (STS) gene expression and regulation in normal human ovarian surface epithelium (OSE) and epithelial ovarian cancer (EOC)

Harlow Christopher R , Wu Xuan , Ren Xia , Fegan Scott , Rae Michael , Price Deborah , Mason J Ian , Hillier Stephen G

At least 90% of human ovarian cancers originate in the OSE. Most studies indicate that EOC is oestrogen responsive. Paradoxically, ovarian cancer generally occurs after the menopause, which raises the question if oestrogen is involved, where does it come from? We hypothesise that the high circulating concentrations of conjugated (inactive) oestrogens in post-menopausal women are substrates for formation of active oestrogen in the OSE through the hydrolytic enzyme activity of S...