Searchable abstracts of presentations at key conferences in endocrinology

ea0015p202 | Growth and development | SFEBES2008

Attempted detection of recombinant glycosylphosphatidylinositol-anchored proteins using Fluorescently Labelled Aerolysin, FLAER

Kaabi Yahia , Wilkinson Ian , Richards Gareth , Skerry Tim , Ross Richard

Introduction: Glycosylphosphatidylinositol (GPI) anchored proteins are common on the surfaces of eukaryotic cells. An example is alkaline phosphatase. Fluorescently Labelled Aerolysin (FLAER) is a bacterial toxin produced by the gram-negative bacterium Aeromonas hydrophila that binds naturally to GPI anchored proteins and utilizes them as receptors to cause cytotoxicity. FLAER has been used to discriminate the GPI deficient red blood cells in cases of paroxysmal nocturn...