Searchable abstracts of presentations at key conferences in endocrinology

ea0031p186 | Obesity, diabetes, metabolism and cardiovascular | SFEBES2013

C-type natriuretic peptide down regulates interferon γ mediated pro-inflammatory gene expression in human endothelium

Day Amy , Fowkes Robert , Lawson Charlotte

Cardiovascular diseases account for more deaths in the Western world than from any other cause. Atherosclerosis has a chronic inflammatory component involving Th1 pro-inflammatory cytokines such as IFN-γ, which is known to induce endothelial cell inflammatory responses. CNP, acting via its receptors to elevate intracellular cGMP, is produced by endothelium and endocardium and is upregulated in atherosclerosis. It is believed to be protective yet its role in vascular infla...

ea0034p155 | Cytokines and growth factors | SFEBES2014

Modelling hepatic encephalopathy in vitro: molecular and functional consequences of hyperammonaemia in C6 glioma cells

Mirczuk Samantha , Robson Camille , Stacey Emily , Fowkes Robert , Lipscomb Victoria

Hepatic encephalopathy (HE), a syndrome of neurological abnormalities caused by impaired liver function, is induced by many chronic liver diseases, such as liver failure or hepatic portosystemic shunts. Whilst multiple substances reach toxic levels in the systemic circulation of HE patients, hyperammonemia (excessive ammonia) is known to be a major contributing factor to the neurological disturbances. Astrocytes utilise ammonia in generating glutamine leading to brain oedema a...

ea0034p163 | Growth and development | SFEBES2014

C-type natriuretic peptide regulates growth and pituitary development in the zebrafish (Danio rerio)

Lessey Andrew , Mirczuk Samantha , McGonnell Imelda , Fowkes Robert

C-type natriuretic peptide (CNP), the predominant natriuretic peptide in the pituitary, is associated with neuroendocrine development and function. Our previous studies show that genes encoding CNP (nppc2, cnp3, nppc4, and nppcl) are expressed during pituitary development in humans and zebrafish (Danio rerio), however a precise role for CNP in the pituitary has yet to be elucidated. Using the versatile zebrafish, we explored in ...

ea0031p140 | Growth and development | SFEBES2013

Comprehensive spatio-temporal expression profiling reveals a complete natriuretic peptide system in the developing Zebrafish (Danio rerio)

Lessey Andrew , Mirczuk Samantha , McGonnell Imelda , Fowkes Robert

C-type natriuretic peptide (CNP) is the ancestral and most highly conserved member of the mammalian natriuretic peptide family. Although broadly expressed throughout the periphery (bone, gonads, kidneys), CNP is the major natriuretic peptide of the brain and CNS and is suspected to influence neuroendocrine function. Our recent studies using fetal human pituitary samples has shown that the gene encoding CNP, NPPC, is expressed early on in pituitary development but a co...

ea0031p248 | Pituitary | SFEBES2013

Transcriptional regulation of C-type natriuretic peptide (CNP/Nppc) and its receptor guanylyl cyclase-B (GC-B/Npr2) in gonadotroph and somatolactotroph cell lines

Mirczuk Samantha , Jones Alexander , McGonnell Imelda , Fowkes Robert

C-type natriuretic peptide (CNP) has recently been implicated as a key meiotic arrest factor in oocytes, and mechanistic studies suggest that the transcriptional regulation of the CNP gene (Nppc) and of its receptor, GC-B (Npr2) is sensitive to gonadotrophin-dependent cAMP accumulation. We have shown CNP to be a major regulator of gonadotrophs in the pituitary, but have yet to establish how either Nppc or Npr2 are transcriptionally controlled locally. In the current study, the...

ea0021p140 | Diabetes and metabolism | SFEBES2009

The impact of thiazolidinediones on microalbuminuria in adults with type 2 diabetes mellitus: a systematic review

Patel Mayur , Fowkes Robert , Sampson Mike , Holland Richard

Background: Microalbuminuria is an early marker of diabetic nephropathy in type 2 diabetes mellitus (T2DM) which predicts the development of later stages of nephropathy. Thiazolidinediones are a class of oral hypoglycaemic drugs used in diabetes glycaemic management that act through PPARγ receptors. Studies have suggested they significantly reduce urinary albumin excretion; however, most trials were small. We therefore performed a systematic review to clarify the effectiv...

ea0021p355 | Steroids | SFEBES2009

Salivary annexin 1 has a diurnal rhythm but does not share an awakening response with cortisol at 30 min

Renshaw Derek , Korbonits Marta , Flower Rod , Perretti Mauro , Fowkes Robert

Several hormones, including cortisol, have a circadian rhythm in plasma and saliva. Salivary cortisol is now extensively used for screening and following patients with various conditions where the regulation of hypothalamo-pituitary–adrenal axis is abnormal, for example in major depression where salivary cortisol levels show a disruption or loss of the circadian rhythm.Annexin 1 is a glucocorticoid-induced protein, which is responsible for mediating...

ea0038p373 | Reproduction | SFEBES2015

Identification of novel transcription factors that may regulate transcription of the equine chorionic gonadotrophin beta subunit

Read Jordan , Cabrera-Sharp Victoria , Mirczuk Samantha , Fowkes Robert , de Mestre Amanda

Equine Chorionic Gonadotrophin (eCG) is a heterodimeric glycoprotein produced by terminally differentiated, bi/multi-nucleate trophoblast cells in the placenta of horses and humans. It is responsible for the maintenance of early pregnancy via rescue of the corpus luteum and subsequently promotion of progesterone production. The beta subunit of eCG is expressed at levels ten-fold lower than that of the alpha subunit and confers the glycoproteins specificity to the placenta. Ver...

ea0034s7.2 | Natriuretic peptides: so much more than salt and water | SFEBES2014

Natriuretic peptides in pituitary development and function

Fowkes Robert , Mirczuk Samantha , Lessey Andrew , Lipscomb Victoria , Niessen Stijn , McArdle Craig , McGonnell Imelda

The cardiovascular properties of the natriuretic peptide family of hormones are well-characterised, and provide valuable prognostic indicators in the treatment of heart failure in humans and companion animals. Of the three main mammalian natriuretic peptides, C-type natriuretic peptide (CNP) remains the most enigmatic, even though CNP represents the ancestral natriuretic peptide from which Atrial- (ANP) and B-type (BNP) natriuretic peptides evolved through gene duplication. We...

ea0034p156 | Cytokines and growth factors | SFEBES2014

Spatial expression profiling reveals tissue-specific sexual dimorphism of the natriuretic peptide system in mice

Mirczuk Samantha , Robson Camille , Robinson Benjamin , Lessey Andrew , Lipscomb Victoria , McGonnell Imelda , Fowkes Robert

The mammalian natriuretic peptide family consists of three members, Atrial-, B-type, and C-type natriuretic peptides (ANP, BNP, and CNP respectively), and exert their effects on blood pressure, sodium homeostasis, growth and reproduction, via their selective guanylyl cyclase receptors, GC-A or GC-B. Previous attempts to map spatial expression profiling have generated conflicting results, due predominantly to the range of techniques employed, and the relative paucity of tools t...