Searchable abstracts of presentations at key conferences in endocrinology

ea0011p345 | Diabetes, metabolism and cardiovascular | ECE2006

Adipose glucocorticoid synthesis: transcription of the 11β-hydroxylase gene in omental but not subcutaneous adipose tissue

MacKenzie SM , Kenyon CJ , Livingstone DEW , Andrew R , Fraser R , Connell JMC , Davies E

The major glucocorticoids (cortisol in man and corticosterone in the rat) play an important role in obesity and its attendant hypertension. However, there is no consistent evidence of glucocorticoid excess in simple obesity, suggesting that its role in the regulation of adipose tissue mass results from a more complex mechanism than adrenal hypersecretion of glucocorticoid. Glucocorticoid biosynthesis is not limited to the adrenal cortex; mRNA and enzymatic activity of 11β...

ea0011p399 | Diabetes, metabolism and cardiovascular | ECE2006

Adrenocortical function in obese Zucker rats

Kenyon CJ , Livingstone DEW , McNeilly AD , Davies E , Andrew R , MacKenzie SM

Adrenal steroids and alterations in steroid metabolism are important factors in the development of obesity in Zucker rats. One might predict, therefore, that the function of the adrenal gland differs between lean and obese Zucker animals. Accordingly, we investigated adrenal gland morphology and function and have considered not only glucocorticoids and HPA activity but also the renin-angiotensin-aldosterone system. Plasma samples and adrenal glands were obtained from young 12 ...

ea0011p752 | Steroids | ECE2006

Alterations in Scavenger Receptor B1 and steroidogenic enzymes within the adrenal gland in response to dietary bile acids

McNeilly AD , Livingstone DEW , MacKenzie SM , Davies E , Kenyon CJ , Walker BR , Andrew R

Bile acids inhibit glucocorticoid and mineralocorticoid metabolism. However bile acids may also influence hormone action by modulating steroidogenesis in the adrenal gland. In rodents adrenal cholesterol supply for steroidogenesis is derived from circulating HDL through the actions of scavenger receptor SR-B1. Since hepatic SR-B1 expression is known to be regulated by bile acids, we have investigated whether adrenal SR-B1 is similarly affected and, if so, whether adrenal lipid...

ea0056oc8.3 | MicroRNAs as biomarkers in endocrine diseases | ECE2018

Circulating levels of microRNAs associate with blood pressure and left ventricular mass in primary hypertensive patients

van Kralingen J.C. , Larsen C.K. , Connell J.M. , Freel E.M. , Zennaro M.C. , MacKenzie S.M. , Davies E.

Introduction: MicroRNA (miRNA) has been shown to post-transcriptionally regulate physiological systems modulating blood pressure, including the adrenal biosynthesis of aldosterone. This raises the possibility that levels of specific miRNAs circulating in plasma might reflect these functional effects and have diagnostic value in the identification of hypertension and its various underlying causes. In a previous study, we measured plasma levels of miRNAs originating from the miR...

ea0011oc44 | Endocrine genetics | ECE2006

Functional impact of polymorphic variation in the gene encoding 11β-hydroxylase (CYP11B1): reduced adrenal 11-hydroxylase efficiency identifies a key intermediate phenotype in hypertension

Barr M , Friel E , MacKenzie SM , Holloway CD , Freel EM , Brain NJR , Wilkinson DM , Ingram M , Fraser R , Dominiczak AF , Connell JMC , Davies E

The regulation of aldosterone secretion is altered in essential hypertension: the phenotype of relative aldosterone excess is present in up to 15% of subjects. The gene encoding aldosterone synthase (CYP11B2) offers an obvious candidate to account for this and a polymorphism in its 5′ untranslated region (UTR; −344C/T) is associated with increased frequency of hypertension and higher aldosterone levels. However, this variation is more closely associated with...