Searchable abstracts of presentations at key conferences in endocrinology

ea0041ep137 | Calcium and Vitamin D metabolism | ECE2016

Mass Spectrometry coupled with liquid chromatography (HPLC-MS-MS) levels of 25-hydroxy vitamin D (25OHVitD) are associated with prognostic markers of Heart Failure

Saponaro Federica , Saba Alessandro , Frascarelli Sabina , Marcocci Claudio , Cetani Filomena , Passino Claudio , Zucchi Riccardo

Introduction and aims: Heart failure (HF) is a health problem with poor prognosis, despite many treatments available. Vitamin D is the pre-hormone of the active calcitriol (1,25(OH)2D3). It is involved in bone homeostasis, but recent studies suggest extraskeletal functions, including pleiotropic effects on cardiovascular system and a relationship between low levels of 25OHvitamin D and worse HF prognosis. The aims of the study were: 1) to define 25OH-D levels in the HF populat...

ea0070ep418 | Thyroid | ECE2020

3-iodothyronamine (T1AM) is taken up and rapidly metabolized in cell culture medium only at low concentration

Saponaro Federica , Borsò Marco , Verlotta Sara , Bandini Lavinia , Saba Alessandro , Ghelardoni Sandra , Zucchi Riccardo

3-iodothyronamine (T1AM) is rapidly catabolized to iodothyroacetic acid after few hours by serum proteins. To avoid a rapid metabolism during any treatment, DMEM devoid of FBS has been used, inducing changes in the cell culture environmental. We evaluated the uptake and the metabolism of different concentrations of T1AM, in presence of supplemented medium, and the presence of 3-iodothyroacetic aldehyde (Ald), a putative intermediate of T1AM catabolism to TA1.<p ...

ea0084ps2-09-85 | Thyroid Hormone ACTION | ETA2022

3-Iodothyroacetic acid release in a mouse model of ischemia-induced synaptic dysfunction

Ricardi Caterina , Bertolini Andrea , Tozzi Francesca , Polini Beatrice , Rutigliano Grazia , Zucchi Riccardo , Chiellini Grazia , Saba Alessandro , Origlia Nicola

The endogenous thyroid hormone derivative 3-iodothyronamine (T1AM), and its metabolite 3-iodothyroacetic acid (TA1) are known to stimulate learning and induce hyperalgesia in mice. Recently, it has been demonstrated that exogenous T1AM is able to rescue synaptic impairment after transient ischemia in the entorhinal cortex (EC), a brain area crucially involved in learning and memory, and early affected during Alzheimer’s disease. However, i...

ea0063gp180 | Benign Thyroid Disorders | ECE2019

Urinary concentration of iodotyrosines correlates with the severity of iodine deficiency in dehal1 knockout mice

Gonzalez-Guerrero Cristian , Borso Marco , Garcia-Gimenez Jorge , Salas-Lucia Federico , Alikhani Pouya , Saba Alessandro , Zucchi Riccardo , Moreno Jose C

Thyroid hormone (TH) synthesis requires iodine, a scarce element whose recycling is mediated by DEHAL1 through deiodination of iodotyrosines MIT and DIT. In humans, DEHAL1 defects lead to severe congenital hypothyroidism (CH) non-detected by neonatal screening programs, which involves the risk of mental retardation in infants. The timing for establishment of this hypothyroidism remains unknown, but environmental iodine deficiency may represent a triggering factor. Whi...

ea0032p991 | Thyroid (non-cancer) | ECE2013

Quantification of tissue T3 and T4 in rat and human heart by a novel HPLC-MS/MS method

Saba Alessandro , Colligiani Daria , Nannipieri Monica , Kusmic Claudia , Dos Remedios Cristobal , Simonides Warner , Iervasi Giorgio , Zucchi Riccardo

Thyroid hormone (T3) is mostly produced in peripheral tissues by thyroxine (T4) deiodination, and its functional effects are directly related to tissue concentration, which is quite difficult to assay by conventional immunological methods. We report a novel technique, based on tandem mass spectrometry (MS/MS) coupled to HPLC, which was used in left ventricular myocardium obtained from rat and human hearts.Samples were homogenized in...

ea0056p234 | Calcium &amp; Vitamin D metabolism | ECE2018

Quantification of serum 25-hydroxyvitamin D: a comparison between competitive chemiluminescence immunoassay and mass spectrometry coupled to high performances liquid chromatography

Saponaro Federica , Frascarelli Sabina , Saba Alessandro , Prontera Concetta , Clerico Aldo , Scalese Marco , Passino Claudio , Marcocci Claudio , Zucchi Riccardo

Serum 25-hydroxy-vitamin D (25OHD) is considered the most reliable marker of vitamin D status. Adequate levels 25OHD of are necessary for pleiotropic effects of vitamin D, either skeletal or extra-skeletal. Traditional assays based on immunoassay often show an unsatisfactory accuracy and sensibility. A valuable alternative is Tandem Mass Spectrometry coupled to High Performances Liquid Chromatography (HPLC-MS-MS), that offers a good quantification accuracy, as the contribution...

ea0073aep802 | Late Breaking | ECE2021

Hypovitaminosis D in patients with SARS-CoV2: Correlation with inflammatory markers and severity of the disease

Saponaro Federica , Franzini Maria , Okoye Chukwuma , Antognoli Rachele , Monzani Fabio , Celi Alessandro , Zucchi Riccardo , Paolicchi Aldo , Saba Alessandro

In March 2020, the infection due to COVID-19 spread as a pandemic emergence showing a mutable phenotype ranging from asymptomatic to lethal severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Among multiple biological and environmental investigated factors, vitamin D status was proposed as a credible candidate, since hypovitaminosis D could be identified as a modifiable risk factor and a potential tool in SARS-CoV-2 ...

ea0084op-08-38 | Oral Session 8: Basic 2 | ETA2022

Brain effects of combined levothyroxine (T4) and 3-iodothyronamine (T1AM) replacement therapy in a murine model of hypothyroidism

Bertolini Andrea , Ricardi Caterina , Maria Grittani Nicoletta , Ippolito Chiara , Moscato Stefania , Mattii Letizia , Frascarelli Sabina , Chiellini Grazia , Saba Alessandro , Zucchi Riccardo , Rutigliano Grazia

Adult-onset hypothyroidism is associated with cognitive dysfunction, and a significant fraction of hypothyroid patients still shows persistent disturbances despite standard T4 replacement therapy. Experimental results showed that tissue levels of T4 metabolites, particularly T1AM, are decreased in hypothyroidism and remain low after T4 replacement. Thyroid hormones are important for the promotion of neurogenesis and neuron migration to different areas of the cerebral cortex, i...