Searchable abstracts of presentations at key conferences in endocrinology

ea0049gp46 | Bone & Calcium Homeostasis 2 | ECE2017

Vitamin D correction elevates apolipoprotein levels in a sex-specific manner

Al-Daghri Nasser , Garbis Spiros , Chrousos George , Sabico Shaun , Aljohani Naji , Al-Saleh Yousef

Numerous studies have identified several extra-skeletal health outcomes to be associated with vitamin D deficiency, yet a definitive causal link is yet to be discovered. Our recent 3D LC-nESI-FTMS proteomic analysis among normal and overweight but apparently healthy adult Saudis identified apolipoproteins, a known independent cardiovascular risk factor, as one of the serological molecular signatures that modulate vitamin D levels. The present interventional study aims to compa...

ea0020p384 | Diabetes and Cardiovascular | ECE2009

Retinol binding protein-4 is associated with TNF-α and not insulin resistance in subjects with type 2 diabetes mellitus and coronary heart disease

Al-Daghri Nasser , Al-Attas Omar , Alokail Majed , Draz Hossam , Bamakhramah Ahmed , Sabico Shaun

We studied the association between RBP4 and various markers related to insulin resistance and diabetic complications as well as inflammatory markers in Saudi population suffering from type 2 diabetes and coronary heart disease. Patients with type 2 diabetes were divided into 3 groups according to the type of treatment and involvement of coronary artery disease. Serum TNF-α, insulin, CRP, resistin, leptin and adiponectin were analysed in all samples. RBP4 plasma levels inc...

ea0034p223 | Obesity, diabetes, metabolism and cardiovascular | SFEBES2014

A 3-month low fat diet leads to significant lipid profile improvement in obese T2DM Saudi subjects, without substantial weight loss, and the capacity to manage a damaging high-fat meal challenge more appropriately post intervention

Al-Disi Dara , Al-Daghri Nasser , Khan Nasiruddin , Alsaif Mohammad , Alfadda Assim , Sabico Shaun , Tripathi Gyanendra , McTernan Philip

Background and aims: Current evidence highlights that dietary cholesterol, trans-fatty acids and saturated fatty acids (SFAs) are all known to increase the levels of systemic atherogenic lipoproteins and cardiovascular disease. The aim of this study was to observe the direct effect of dietary change, via a calorie-restricted diet on i) cardio-metabolic profile and ii) a high-fat meal challenge pre- and post-3-month intervention.Methods: T2DM subjects (Sa...

ea0031p188 | Obesity, diabetes, metabolism and cardiovascular | SFEBES2013

Human abdominal subcutaneous adipocytes as an active source of LpPLA2, influenced by fat depot and metabolic state, with LpPLA2 converting LDL into more potent atherogenic Ox-LDL, in vitro

Kumsaiyai Warunee , Harte Alison , Al-Naji Fadi , Al-Daghri Nasser , Kyrou Ioannis , Barber Thomas , Sabico Shaun , Tripathi Gyanendra , McTernan Philip

Lipoprotein-associated phospholipase A2 (LpPLA2) is a member of the phospholipaseA2 super family of enzymes, and is upregulated in arterial inflammation, obesity and cardiovascular disease. The other isoforms, iPLA2 and cPLA2, appear to contribute to inflammation through production of lipid mediators. The role of PLA2 in human adipose tissue (AT) is unclear, therefore we sought to i) characterise PLA2 isoforms in lean, obese, T2DM abdominal subcutaneous (AbdSc) and omental (Om...

ea0031p195 | Obesity, diabetes, metabolism and cardiovascular | SFEBES2013

Replication of genome wide association-validated loci for type 2 diabetes mellitus in the Saudi Arabian population

Al-Daghri Nasser , Alkharfy Khalid , Alokail Majed , Alenad Amal , Al-Attas Omar , Mohammad Abdul Khader , Sabico Shaun , Albagha Omar

Background: Previous genome wide association studies in Caucasian and South Asian populations have identified over 35 loci for type 2 diabetes mellitus (T2DM) risk. However, little is known about the contribution of these loci in T2DM from a Saudi Arabian population. In this study we investigated for the first time, the association of 38 previously identified T2DM risk loci (32 loci from Caucasian and six loci from South Asian populations) in 1166 T2DM patients and 1235 health...

ea0031p222 | Obesity, diabetes, metabolism and cardiovascular | SFEBES2013

Vitamin D supplementation as influenced by diabetic therapies

Alkharfy Khalid , Al-Daghri Nasser , Al-Othman Abdulaziz , Moharram Osama , Alokail Majed , Al-Saleh Yousef , Sabico Shaun , McTernan Philip

Objective: Vitamin D deficiency is associated with an increased risk of type two diabetes mellitus (T2DM) and cardiovascular disease reduced through Vitamin D3 supplementation. However to the best of our knowledge, no research has determined the effect of vitamin D3 supplementation in conjunction with existing pharmacological and non-pharmacological approaches to the DMT2 population. Hence, the aim of this study was to determine the effect of vitamin D3 supplementation in a co...

ea0049gp77 | Diabetes & complications 1 | ECE2017

Susceptibility to type 2 diabetes may be modulated by haplotypes in G6PC2, a target of positive selection

Clerici Mario , Al-Daghri Nasser , Pontremolli C , Cagliani R , Forni Diego , Alokail Majed , Al-Attas Omar , Sabico Shaun , Riva S , Sironi M

Introduction: The endoplasmic reticulum enzyme glucose-6-phosphatase catalyzes the common terminal reaction in the gluconeogenic and glycogenolytic pathways and plays a central role in glucose homeostasis. In most mammals, different G6PC subunits are encoded by three paralogous genes (G6PC, G6PC2, and G6PC3). Mutations in G6PC and G6PC3 are responsible for human Mendelian diseases, whereas variants in G6PC2 are associated with fasting glucose (FG) levels.<p class="abstext"...

ea0028oc3.2 | Obesity, thyroid and Addison's disease | SFEBES2012

Post-prandial high fat intake leads to acute exposure to circulating endotoxin in type 2 diabetes mellitus subjects

Harte Alison , Varma Madhusudhan , Tripathi Gyanendra , McGee Kirsty , Al-Daghri Nasser , Al-Attas Omar , Sabico Shaun , O'Hare Joseph , Ceriello Antonio , Saravanan Ponnusamy , Kumar Sudhesh , McTernan Philip

Habitual post-prandial absorption of saturated fatty acids (SFAs) increases systemic atherogenic lipoproteins and a pro-inflammatory response, a condition exacerbated by obesity, insulin resistance (IR) and type 2 diabetes mellitus (T2DM). Also, gut derived bacteria (endotoxin) is absorbed coupled to damaging lipoproteins as it crosses the mucosa, which could directly impact on adipose tissue, as our previous studies suggest. The current studies addressed whether a SFA rich me...

ea0016p225 | Diabetes and cardiovascular diseases | ECE2008

Circulating endotoxin as a potential biomarker and mediator of inflammation: influenced by diabetic therapies

Al-Daghri Nasser , Al-Rubeaan Khalid , Al-Attas Omar , da Silva Nancy , Sabico Shaun , Kumar Sudhesh , Mc Ternan Philip , Harte Alison

Recent studies suggest that endotoxin from gut flora, may be key to development of inflammation by stimulating the secretion of an adverse cytokine profile from adipose tissue. The study investigated the relationship between endotoxin and various metabolic parameters of diabetic patients to determine if anti-diabetic therapies exerted a significant effect on endotoxin levels and adipocytokine profiles of T2DM subjects. Fasting blood samples were collected from consenting Saudi...