Searchable abstracts of presentations at key conferences in endocrinology

ea0016p670 | Steroid receptors | ECE2008

Altered androgen metabolism and signalling during tissue remodelling of the prostatic stroma: implications for prostate cancer and benign prostatic hyperplasia

Sampson Natalie , Plas Eugen , Berger Peter

The human prostate is highly susceptible to benign and malignant proliferative changes and is associated with the development of benign prostatic hyperplasia (BPH) and prostate cancer (PCa), two of the most common diseases affecting elderly males. The diseased-associated stroma undergoes significant remodelling, including fibroblast-to-myofibroblast/smooth muscle cell transdifferentiation. Subsequent increased secretion of cytokines and growth factors generates a growth favour...

ea0014p9 | (1) | ECE2007

Regulation of GAGEC1, a cancer-testis associated antigen family member, by sex steroid hormones and TGF-beta: implications for prostatic disease

Sampson Natalie , Schuhfried Gerlinde , Plas Eugen , Berger Peter

Prostate homeostasis and function are regulated by complex interactions between the fibromuscular stroma and secretory epithelium via locally-derived and systemic paracrine- and autocrine-acting growth factors and sex steroid hormones. Stromal tissue remodelling due to alterations in transforming growth factor beta (TGF-β) and sex steroid hormone signalling are associated with benign prostatic hyperplasia (BPH) and prostate cancer (PCa), two of the most common proliferati...

ea0042p3 | (1) | Androgens2016

Diminished response of prostate cancer cells to antiandrogens upon co-culture with cancer-associated fibroblasts as shown in a 3-dimensional prostate cancer epithelial-stromal organoid model

Eder Theresa , Weber Anja , Neuwirt Hannes , Grunbacher Georg , Schafer Georg , Ploner Christian , Klocker Helmut , Sampson Natalie , Eder Iris E

The use of more tissue-mimetic 3-dimensional (3D) cell culture models for in vitro drug screening has significantly increased in the past with the expectation to overcome with over-interpretation of drug effects obtained with conventional 2D cultures. In this study, we characterized 3D prostate cancer (PCa) organoids where PCa epithelial cells (LNCaP, DuCaP, LAPC4) were cultured alone or with PCa-associated fibroblasts (CAFs) in 96 well hanging drop plates in order to...