Endocrine Abstracts

The Estrogen Receptor (ER) is a well-studied, ligand dependent transcription factor. We have analysed, by kinetic chromatin immunoprecipitation (ChIP) assays, the mechanisms by which the ER modulates the permissiveness and transcriptional activity of the PS2 gene. Sequential ChIP assays were used to define the cyclical association of approximately 50 different proteins to the pS2 promoter. Key results from these studies are (i) that unliganded and liganded ER direct cycling on...