Searchable abstracts of presentations at key conferences in endocrinology

ea0016p176 | Diabetes and cardiovascular diseases | ECE2008

Adiponectin stimulates monocytic release of CCL2, -3, -4 and -5 concomitantly reduces the surface abundance of CCR1, -2 and -5

Weigert Johanna , Neumeier Markus , Bruhl Hilke , Bollheimer Cornelius , Buttner Roland , Schaffler Andreas , Scholmerich Jurgen , Buechler Christa

Background: Systemic adiponectin is reduced in patients with obesity and type 2 diabetes mellitus. Peripheral monocytes of type 2 diabetic patients are activated and show a higher surface abundance of CCR2. Inflammatory markers like CCL2 are elevated in the serum of these patients. Therefore, the influence of adiponectin on the release of CC chemokines and the abundance of the corresponding receptors were investigated in monocytic cells.Methods: Primary ...

ea0016p494 | Obesity | ECE2008

Gene expression profiling in skeletal muscle from diet-induced obesity susceptible rats

Buttner Roland , Schaffler Andreas , Buchler Christa , Wrede Christian , Scholmerich Jurgen , Bollheimer Cornelius

Background: The variablity of the individual susceptibility to diet-induced obesity and insulin resistance is well known, but so far insufficiently explained. As skeletal muscle metabolism and insulin sensitivity are important pathophysiologic features of obesity-asscociated metabolic perturbations, we tried to identify differences in muscular gene expression profiles between high fat fed rats prone to develop obesity (diet-induced obesity, DIO) and those which remained lean (...

ea0016p495 | Obesity | ECE2008

A pharmacologic sympathectomy prevents the development of obesity and insulin resistance in the high fat fed rat

Bollheimer Cornelius , Harle Peter , Schaffler Andreas , Buchler Christa , Wrede Christian , Straub Rainer , Scholmerich Jurgen , Buttner Roland

Background: Obese patients show an increased activity of the sympathetic nervous system, but the causality of the relationship is not known. Here, we examine whether a pharmacologic sympathectomy influences diet-induced obesity and insulin resistance in the high fat fed rat model.Methods: Wistar-Rats were fed ad libitum with a high fat diet (HF, 40 energy% of fat, based on lard) for 12 weeks. A pharmacologic sympathectomy was performed by a) weekl...

ea0016oc3.4 | Diabetes and obesity | ECE2008

Fatty acid-induced induction of TLR-4/NFκB-pathway in adipocytes links nutritional signaling with innate immunity

Schaffler Andreas , Gross Philipp , Buttner Roland , Bollheimer Cornelius , Buchler Christa , Neumeier Markus , Kopp Andrea , Scholmerich Juergen , Falk Werner

Objectives: To study the direct effects of fatty acids (FA) and the involvement of the TLR-4/NFκB pathway with respect to the secretion of adipokines and chemokines from adipocytes.Research design and methods: 3T3-L1 adipocytes were stimulated with increasing doses of FA. The secretion of adiponectin, resistin and MCP-1 was measured by ELISA. NFκB p65 nuclear translocation and TLR-4 expression were investigated by Western blot analysis. NF&#954...

ea0016p490 | Obesity | ECE2008

Adiponectin induces CXCL8 in primary human hepatocytes via the adiponectin receptor 1

Wanninger Josef , Neumeier Markus , Weigert Johanna , Weiss Thomas , Bollheimer Cornelius , Buettner Roland , Schaffler Andreas , Scholmerich Jurgen , Buechler Christa

Background: Adiponectin was suggested to exert hepatoprotective effects in animal models of tetrachlorcarbonate-, endotoxin- and alcohol-induced liver injury. Further, in patients with non-alcoholic fatty liver disease (NAFLD) low adiponectin levels were found to be associated with more severe steatosis and hepatic inflammation.Methods: To investigate the molecular basis of the hepatoprotective effects of adiponectin primary human hepatocytes were incuba...