Searchable abstracts of presentations at key conferences in endocrinology

ea0081ep362 | Diabetes, Obesity, Metabolism and Nutrition | ECE2022

Imidazole-osmium reduces elution of lipids from cryofixed rat hepatic tissue for ultrastructural analysis

Durr Sabine , Reipert Siegfried , Krššak Martin , Philippe Cecile , Ehret Viktoria , Luft Matthias , Schintlmeister Arno , Scherer Thomas , Furnsinn Clemens

Introduction: Transmission electron microscopy (TEM) is the main tool for studying the ultrastructural properties of metabolically diseased tissue. For impactful micrographs as well as for interpretation of TEM specimens, the integrity of cellular components is crucial. Elution of substances is a major problem in TEM preparation with no universal solution found so far. Investigating fatty liver disease in a rat model, an important factor is the depiction of undamaged lipid dro...