Searchable abstracts of presentations at key conferences in endocrinology

ea0035p827 | Pituitary Basic (<emphasis role="italic">Generously supported by IPSEN</emphasis>) | ECE2014

Effect of ubiquitin/proteosome inhibition on acth secretion by rat corticotropes

Sesta Antonella , Cassarino Maria Francesca , Cavagnini Francesco , Giraldi Francesca Pecori

ACTH is the product of proteolytic processing of its precursor, proopiomelanocortin (POMC) and, in turn, is subject to proteolytic degradation. Regulation of proteolysis is crucial to several intracellular processes, including protein activation and inactivation, and thus contributes to active peptide concentrations. The proteosome 26S/ubiquitin system (UPS) is the main, non-lisosomial intracellular proteolytic pathway and alterations to this system have been observed in both ...

ea0037ep15 | Adrenal cortex | ECE2015

The effect of retinoic acid on human adrenal corticosteroid secretion in vitro

Sesta Antonella , Tapella Laura , Cassarino Maria Francesca , Castelli Luigi , Cavagnini Francesco , Giraldi Francesca Pecori

Retinoic acid, a derivative of vitamin A, has recently yielded promising results in the treatment of Cushing’s disease (Pecori Giraldi et al. JCEM 2012). Its main site of action appears to be the tumoural corticotrope as retinoic acid inhibits POMC transcription and corticotrope proliferation (Paez-Pereda et al. JCI 2001). Studies on tumoural adrenal cell lines have revealed an additional inhibitory effect on cell proliferation and stimulated co...

ea0037ep657 | Pituitary: basic and neuroendocrinology | ECE2015

Effect of phenols and phthalates on rat and human pituitary cell proliferation

Tapella Laura , Sesta Antonella , Losa Marco , Cavagnini Francesco , Giraldi Francesca Pecori

An increased prevalence of acromegaly and pituitary tumors has recently been observed in high industrial density areas (Cannavò et al. J Endocrinol 2010) or after dioxin exposure (Pesatori et al. Eur J Endocrinol 2008). These data as well as vitro studies on endocrine disruptors (EDs) suggest that environmental pollutants may affect hormonal secretion and pituitary cell proliferation (Blake et al. Proc Soc Exp Biol...

ea0070aep602 | Pituitary and Neuroendocrinology | ECE2020

Effect of silibinin on acth synthesis and secretion in human adenomatous corticotropes in vitro

Francesca Cassarino Maria , Sesta Antonella , Libera Laura , Bardelli Donatella , Losa Marco , Lasio Giovanni , Pecori Giraldi Francesca

Silibinin, a milk thistle extract with known hepatoprotective effects, has recently been shown to act upon tumoral corticotropes and revert the Cushingoid phenotype in an allograft mouse model (Riebold et al. 2015). Silbinin is known to inhibit HSP90 -a chaperone to the glucocorticoid receptor-thereby restoring sensitivity to glucocorticoid negative feedback in tumoral corticotropes. Aim of the present study was to assess the effect of silibinin on ACTH synthesis and ...

ea0073aep15 | Adrenal and Cardiovascular Endocrinology | ECE2021

Effect of retinoic acid on adrenal primary cultures from patients with Cushing’s disease

Giraldi Francesca Pecori , Sesta Antonella , Tapella Laura , Cassarino Maria Francesca , Castelli Luigi

Retinoic acid, a major modulator of adrenal development and differentiation, has also been shown to inhibit ACTH secretion by tumoral corticotropes. Recent clinical trials in patients with Cushing’s disease revealed that retinoic acid exerts beneficial effects in these patients (1, 2). Of note, the decrease in cortisol secretion during retinoic acid administration was more pronounced than the change in ACTH levels (1) suggesting a direct action at adrenal level. Aim of th...