Searchable abstracts of presentations at key conferences in endocrinology

ea0012p95 | Reproduction | SFE2006

Characterisation of 11β-hydroxysteroid dehydrogenase (11βHSD) expression and activity in the pre- and post-pubertal porcine (sus scrofa) testis and male reproductive tract

Sharp V , Fowkes RC , Thurston LM , Michael AE

Cortisol is metabolised by 11β-hydroxysteroid dehydrogenase enzymes (11βHSD1 and 11βHSD2). In immature rat Leydig cells, 11βHSD1 acts as an oxo-reductase whereas, after puberty, the dehydrogenase activity of 11βHSD1 predominates. In adult rats, 11βHSD1 is also highly expressed in caput epididymis, vas deferens and vesicular glands. Recently, 11βHSD1 mRNA and activities have been reported in post-pubertal pig testis. The objective of this stud...

ea0011p717 | Reproduction | ECE2006

11β-hydroxysteroid dehydrogenase (11βHSD) activities in porcine granulosa cells from ovarian follicles and cysts

Sunak N , Sharp V , Abeydeera LR , Thurston LM , Michael AE

In the ovary, glucocorticoids are metabolised by 11βHSD enzymes. The bi-directional 11βHSD1 enzyme usually acts as an NADPH-dependent 11-ketoreductase to regenerate cortisol (F) from circulating cortisone (E), while 11βHSD 2 inactivates F using NAD+ as cofactor. Having isolated endogenous inhibitors of 11βHSD1 from porcine follicular fluid (pFF) and ovarian cysts, the aims of this study were to establish whether 11βHSD enzymes interconvert F...

ea0010p66 | Reproduction | SFE2005

11β-hydroxysteroid dehydrogenase (11βHSD) activities in the testis and reproductive tract of post-pubertal boars

Sharp V , Jonas K , Sunak N , Michael A , Thurston L

The enzymatic inactivation of cortisol is catalysed by isoforms of 11βHSD. 11βHSD1 is a bi-directional, NADP(H)-dependent enzyme whereas11βHSD2 is an NAD+-dependent dehydrogenase that inactivates cortisol. In the male rat, 11βHSD1 is highly expressed in the testis, epididymis, vas deferens and vesicular glands. Although 11βHSD activities have recently been reported in boar testicular tissue, cortisol metabolism in the boar reproductive tract...

ea0010p68 | Reproduction | SFE2005

Intra-follicular cortisol:cortisone (f:e) ratios in porcine antral follicles and ovarian cysts; relationship to ovarian modulators of type 1 11β-hydroxysteroid dehydrogenase (11βHSD1) activity

Sunak N , Sharp V , Wood P , Abeydeera L , Thurston L , Michael A

In the ovary, 11βHSD enzymes inter-convert cortisol (F) with its inert metabolite, cortisone (E). Porcine follicular fluid from antral follicles and spontaneous ovarian cysts contains hydrophobic compounds that inhibit NADP(H)-dependent cortisol metabolism by 11βHSD1. The aim of this study was to measure intra-follicular F:E ratios in porcine antral follicles and ovarian cysts, and determine whether F:E ratios correlate with the inhibition of 11βHSD1 by porcine ...