Endocrine Abstracts

Hormone-responsive reporter plasmids are widely used for the analysis of endocrine responses in target cells. We have investigated the effect of different promoters on reporter gene expression by oestrogen-responsive reporter constructs.Plasmid constructs were transfected into an oestrogen responsive bovine endometrial stromal (BST) cell line. Two reporter gene products were analysed: secreted alkaline phosphatase(SeAP) and beta galactosidase.<p clas...