Searchable abstracts of presentations at key conferences in endocrinology

ea0038p301 | Pituitary | SFEBES2015

Investigation of the invasive phenotype of AIP-mutated pituitary adenomas

Stiles Craig , Barry Sayka , Gadaleta Emanuela , Chelala Claude , Shoulders Carol C , Korbonits Marta

Background: Heterozygous germline AIP mutations can lead to young-onset invasive GH-secreting adenomas. There are no data available to explain the proliferative and invasive nature of AIP-mutation positive somatotrophinomas.Methods: Cell viability (MTS assay), invasion (single cell fluorescence invasion assay) and migration assays (Boyden chambers) were used to further characterise the phenotype of AIP-silenced GH3 cells. Affymetrix gen...

ea0038p307 | Pituitary | SFEBES2015

Silencing of aryl hydrocarbon receptor protein (AIP) up-regulates the small Rho GTPase, CDC42

Mothojakan Nadira B , Stiles Craig E , Barry Sayka , Shoulders Carol C , Korbonits Marta

: AIP mutation-positive familial isolated pituitary adenoma is commonly diagnosed in young patients who have a poor prognosis due to large, treatment-resistant tumours. Microarray analysis carried out on AIP knockdown pituitary (GH3) cells and control cells, identified CDC42 as one of the genes that was up-regulated by loss of AIP protein. This small Rho GTPase activates MAPK signalling, suggesting it may contribute to the proliferative phenotype of AIP knock...