Searchable abstracts of presentations at key conferences in endocrinology

ea0022p679 | Obesity | ECE2010

Differential expression of proteins in omental and subcutaneous adipose tissue

Insenser Maria , Montes Rafael , Simo Rafael , Vendrell Joan , Escobar-Morreale Hector F

Background: Adipose tissue is considered a highly active metabolic and endocrine organ with important physiological functions. The evaluation of protein expression has the potential advantage of providing a global vision of the cellular pathways associated with adipose tissue dysfunction and obesity, permitting the identification of novel candidate molecules that would have not been considered of interest otherwise.Objectives: To identify proteins differ...

ea0056pl4 | The Retina as a Window for Exploring the Brain in Diabetes | ECE2018

The Retina as a Window for Exploring the Brain in Diabetes

Simo Rafael

Evidence is accumulating that type 2 diabetes (T2D) is associated with cognitive impairment and dementia. In fact, numerous epidemiological studies have demonstrated that T2D patients have a significantly higher risk of developing neurodegenerative diseases and, in particular, Alzheimer’s disease (AD). In clinical practice there are no reported phenotypic indicators or specific examinations to identify T2D patients at risk of developing AD. This gap should be urgently bri...

ea0037ep602 | Obesity and cardiovascular endocrinology | ECE2015

SHBG-C57BL/ksJ-db/db: a new mouse model to study the link between SHBG regulation and obesity development

Lopez Cristina Saez , Hernandez Cristina , Simo Rafael , Selva David Martinez

Low plasma SHBG levels in overweight individuals are a biomarker for the metabolic syndrome and are predictive of type 2 diabetes and risk of cardiovascular disease. There are no in vivo models to study SHBG expression and regulation during obesity development. The main reason for this is that the obesity-prone rodent models cannot be used to study this issue since rodents unlike humans do not express the SHBG gene in their livers. We have developed by crossi...

ea0035p177 | Cardiovascular Endocrinology & Lipid Metabolism | ECE2014

Red wine increases SHBG production by HepG2 cells

Lopez Cristina Saez , Simo Rafael , Hernandez Cristina , Selva David Martinez

Low circulating sex hormone-binding globulin (SHBG) is an independent risk factor for cardiovascular disease. Mediterranean diet has been associated with a decreased risk of cardiovascular disease. We have recently shown that olive oil consumption is associated with elevated SHBG serum levels and that PPARγ downregulation induced by oleoyl-CoA is an important underlying mechanism of such regulation. Red wine is also an important component of the Mediterranean diet and its...

ea0032p205 | Cardiovascular Endocrinology & Lipid Metabolism | ECE2013

Oleic vs linoleic effects on hepatic sex hormone-binding globulin production

Lopez Cristina Saez , Hernandez Cristina , Simo Rafael , Selva David M

Low plasma SHBG is an independent risk factor of cardiovascular disease. Our recent work has shown that SHBG gene expression can be regulated indirectly through HNF4α gene expression. In this regard, we have identified that an increase in hepatic palmitate induced by high carbohydrate diets and pro-inflammatory cytokines (TNFα and IL1β) was able to reduce hepatic HNF4α protein levels which, in turn, decreased SHBG production. The Mediterranean diet, in whic...

ea0032p729 | Obesity | ECE2013

New insights on molecular mechanisms regulating hepatic sex hormone-binding globulin production: clinical implications in obesity and type 2 diabetes

Saez-Lopez Cristina , Hernandez Cristina , Simo Rafael , Selva David M

Human sex hormone-binding globulin (SHBG) is produced and secreted by the liver and it binds androgens and estrogens with high affinity. In blood, SHBG acts as a carrier of these sex steroids and regulates their bioavailability. Low plasma SHBG levels are associated with obesity, abdominal adiposity and metabolic syndrome, and predict the development of type 2 diabetes. In addition, an inverse relationship between plasma SHBG levels and risk of cardiovascular disease has been ...

ea0056gp149 | Obesity | ECE2018

Sex hormone-binding globulin is more than a sex steroid carrier: a New therapeutic target against obesity and non-alcoholic fatty liver disease

Saez-Lopez Cristina , Hernandez Cristina , Simo Rafael , Selva David M

Low plasma SHBG levels are present in patients suffering chronic metabolic diseases, including obesity and non-alcoholic fatty liver disease (NAFLD). In overweight individuals, low plasma SHBG levels are a biomarker for the metabolic syndrome and predict a higher risk of suffering type 2 diabetes and cardiovascular disease. Our recent results demonstrate that SHBG is more than sex hormone carrier and its reduction in obese subjects play an active role in obesity and NAFLD deve...

ea0063gp224 | Adrenal and Neuroendocrine - Clinical (1) | ECE2019

Caffeine upregulates hepatic SHBG production by increasing adiponectin in white adipose tissue

Brianso-Llort Laura , Fuertes-Rioja Lidia , Perez Lorena Ramos , Hernandez Cristina , Simo Rafael , Selva David

Epidemiological studies have shown that caffeine increases plasma SHBG levels and also reduce the risk of type 2 diabetes. There are no reports describing any molecular mechanism by which caffeine regulates hepatic SHBG production. The aim of the present study was to explore whether caffeine regulates SHBG production and to determine the associated molecular mechanisms. For this purpose, in vitro and in vivo studies were performed using human HepG2 cells and ...

ea0063p226 | Pituitary and Neuroendocrinology 1 | ECE2019

Metabolic fingerprint of acromegaly and its potential usefulness in clinical practice

Biagetti Betina , Herance JR , Ferrer Roser , Aulinas Anna , Mesa Jordi , Simo Rafael

Background: Acromegaly is a rare chronic and debilitating disease. More than half of patients are not well controlled and most of them require chronic surveillance. Although IGF1 and GH circulating levels are the main targets, they are not in close relationship with the behaviour of the tumour, the clinical course of the disease, the therapy response and the associated comorbidities. Metabolomics is an emergent research tool that we have used to examine in a massive manner the...

ea0049ep753 | Cardiovascular Endocrinology and Lipid Metabolism | ECE2017

Resveratrol increases hepatic SHBG expression through human constitutive androstane receptor: a new Contribution to the French Paradox

Saez-Lopez Cristina , Brianso-Llort Laura , Torres-Torrenteras Javier , Simo Rafael , Hammond Geoffrey , Selva David

Sex hormone-binding globulin (SHBG) carries sex steroids in blood regulating their bioavailability. Red wine consumption increases plasma SHBG levels, and we have discovered that resveratrol, a polyphenol enriched in red wine, acts specifically through the human constitutive androstane receptor (CAR), a drug/xenobiotic detoxification gene regulator, to increase hepatic SHBG production. Chromatin immunoprecipitation and luciferase reporter gene assays show that human CAR binds ...