Searchable abstracts of presentations at key conferences in endocrinology

ea0065p166 | Metabolism and Obesity | SFEBES2019

Obesity-induced changes in hepatocyte and skeletal myocyte expression of mRNAs encoding islet GPCR peptide ligands

Atanes Patricio , Smith Lorna , Bowe James , Persaud Shanta

Introduction: Insulin-sensitive tissues such as liver and skeletal muscle modify their gene expression under conditions of obesity-induced insulin resistance, and some of these gene products may be released to maintain glucose homeostasis. This study aimed to identify mRNAs encoding liver and skeletal muscle peptides that have the potential to regulate β-cell function by binding to islet GPCRs, and to quantify changes in expression of these liver and muscle mRNAs in obese...

ea0065p200 | Metabolism and Obesity | SFEBES2019

A role for kisspeptin in long-term islet function in females

Smith Lorna , Hill Thomas , Simpson Sian , Jones Peter , Bowe James

Although characterised predominantly for its role in the brain, the neuropeptide kisspeptin has previously been shown to potentiate glucose-stimulated insulin through its receptor, GPR54; expressed abundantly in β-cells. We have previously reported a physiological role for kisspeptin signalling in the islet adaptation to pregnancy, using a β-cell specific GPR54 knockout mouse line (βGPR54ko). The aim of the present study was to examine the effects of impaired &#...

ea0065p346 | Reproductive Endocrinology and Biology | SFEBES2019

Islet adaptations to pregnancy: a role for Urocortin 2

Simpson Sian , Smith Lorna , Jones Peter , Bowe James

Previous studies have shown beneficial effects of the corticotropin releasing hormone (CRH) family, including the urocortins (UCN1, UCN2 and UCN3), on pancreatic islets and subsequent glucose homeostasis. However, the physiological relevance of this interaction is not currently understood. CRH and urocortins are also expressed by placenta, so this study investigated whether signalling through CRH receptor 1 or 2 (CRHR1/CRHR2) plays a role in the islet adaptation to pregnancy. ...

ea0077lb44 | Late Breaking | SFEBES2021

Depression and islet function during pregnancy: Generation of a depressive phenotype using UCMS

Smith Lorna , Fernandes Cathy , Simpson Sian , Liu Bo , Jones Peter , Bowe James

Gestational diabetes (GDM) occurs when beta-cell insulin secretory capacity is insufficient to meet the increased demands required to maintain normoglycemia during pregnancy. Considerable clinical evidence supports a link between depression and GDM, although underlying mechanisms are unclear. We used the unpredictable chronic mild stress (UCMS) rodent model of depression to examine the metabolic effects of depression in pregnant mice. C57BL/6J females were divided at 4-weeks-o...

ea0038p391 | Steroids | SFEBES2015

Involvement of the TORC isoforms in adrenal responsiveness to basal and stress-induced HPA activity

Smith Lorna , Zhao Zidong , Hazell George , Aguilera Greti , Lightman Stafford , Spiga Francesca

ACTH signalling at the adrenal zona fasciculata induces rapid synthesis of glucocorticoids, accompanied by rapid transcription of genes encoding steroidogenic enzymes, including the labile, rate-limiting protein, StAR protein. ACTH induction of StAR transcription has been demonstrated to be predominantly CREB-dependent, and this mechanism is regulated by both CREB phosphorylation and its binding to the CREB co-activator TORC (transducer of regulated CREB activity, also known a...

ea0025p237 | Pituitary | SFEBES2011

Molecular and functional characterisation of an endocannabinoid system in LβT2 gonadotrophs

Smith Lorna , Davies Elizabeth , Gastaldello Annalisa , Thompson Iain , Perello Edward , Korbonits Marta , Campanella Michelangelo , Fowkes Robert

Cannabinoids are known to exert effects throughout the endocrine system, including in the anterior pituitary. Whilst cannabinoid receptors (CB1 in particular) are expressed in numerous tissues, expression and function of an endocannabinoid system within specific anterior cell types has not been investigated. We have used well-characterised gonadotroph (LβT2 and αT3-1) and somatolactotroph (GH3) cell lines to establish the expression and functional role for cannabinoi...