Searchable abstracts of presentations at key conferences in endocrinology

ea0038p349 | Reproduction | SFEBES2015

Decoding gonadotrophin receptor signalling via spatial regulation of the LH receptor

Sposini Silvia , Jean-Alphonse Frederic , Hanyaloglu Aylin

The LH receptor (LHR) is essential for mediating multiple functions in reproduction and pregnancy. LHR belongs to the superfamily of G-protein-coupled receptors (GPCRs) that impacts nearly every aspect of human physiology and pathophysiology. Membrane trafficking is a critical mechanism for cells to decode complex signalling networks into specific downstream responses, including the signalling pathways activated by GPCRs. A detailed molecular description of GPCR trafficking is...

ea0031p296 | Pituitary | SFEBES2013

Dissecting the prokineticin receptor dimerization interface: a role in kallmann sindrome?

Sposini Silvia , Hanyaloglu Aylin , Miele Rossella

Prokineticin receptors (PKR1 and PKR2) are GPCRs that belong to neuropeptide Y receptor class. They exert their biological functions binding two structurally related peptides (Bv8 or PK2 and EG-VEGF orr PK1). Intensive research over the past few years has shown that PKs/PKRs signalling modulates neuronal survival and neurogenesis, hypothalamic hormone secretion, nociception, circadian rhythm and complex behaviours, such as feeding and drinking. It also promotes angiogenesis in...

ea0086p124 | Reproductive Endocrinology | SFEBES2022

Divergent G-protein signal control at the very early endosome (VEE) from the dually coupled luteinizing hormone receptor (LHR)

Richardson Rachel , Sposini Silvia , Yildiz Yoldas , Barlow Lucy , Hanyaloglu Aylin

Membrane trafficking of G-protein coupled receptors (GPCRs) is a critical mechanism by which cells mediate complex signaling pathways. Endosomes are increasingly recognized as platforms for GPCR signaling. The VEE was first identified using the LHR, a GPCR that is key in reproduction. We have shown that recycling of the LHR is driven by receptor mediated Gαs cAMP signaling from the VEE and PKA-dependent phosphorylation of the adaptor protein, APPL1, while APPL1 negatively...

ea0034p320 | Reproduction | SFEBES2014

Post-endocytic sorting of the LH receptor is mediated by a novel APPL1 dependent mechanism

Sposini Silvia , Jean-Alphonse Frederic , Newcombe Ashley , Hanyaloglu Aylin

LH receptor (LHR) is a G protein-coupled receptor (GPCR) that plays key roles in reproduction and pregnancy. Endocytic trafficking and sorting of GPCRs to diverse cellular fates represent a key mechanism in defining cellular responses by controlling both the temporal and spatial parameters of cellular signalling. How different processes within the endocytic system are coordinated, however, needs to be mechanistically dissected in more detail. We have recently demonstrated that...

ea0044p211 | Reproduction | SFEBES2016

Insight into the molecular mechanisms underlying alterations in gonadotropin receptor activity in polycystic ovarian syndrome

Owens Lisa , Lerner Avi , Sposini Silvia , Christopoulos George , Khanjani Shiran , Islam Razia , Lavery Stuart , Tsui Vicky , Hardy Kate , Franks Stephen , Hanyaloglu Aylin

Introduction: Polycystic ovary syndrome (PCOS) is a common endocrine disorder, affecting 5–10% of women of reproductive age, and is the major cause of anovulatory infertility. Aberrant secretion and/or action of gonadotropins are implicated but, to date, we have only limited knowledge about the precise mechanisms involved. Recent genome wide association studies have discovered signals at loci close to the genes coding for gonadotropin receptors. The functional significanc...