Searchable abstracts of presentations at key conferences in endocrinology

ea0016p522 | Obesity | ECE2008

Glucose-dependent insulinotropic polypeptide downregulates expression and enzyme activity of 11β-Hydroxysteroid Dehydrogenase type 1

Andres Janin , Gogebakan Ozlem , Mai Knut , Pfeiffer Andreas , Spranger Joachim

Introduction: 11β-hydroxysteroid dehydrogenase type 1 (11 β-HSD1) converts cortisone into its active metabolite, cortisol. Mechanisms regulating the promoter-activity of 11β-HSD1 are of considerable importance to understand the basics of intracellular cortisol, lipid and glucose metabolism. The incretine glucose-dependent insulinotropic polypeptide (GIP) has been suggested to affect insulin sensitivity. However, the mechanisms of this effect are unclear yet....

ea0022p142 | Cardiovascular endocrinology and lipid metabolism | ECE2010

Insulin mediates the glucagon-induced decrease in atrial natriuretic peptide: a randomized controlled trial

Arafat Ayman , Rudovich Natalia , Adamidou Aikaterini , Spranger Joachim , Weickert Martin , Mohlig Matthias , Pfeiffer Andreas

Objectives: Atrial natriuretic peptide (ANP) is a cardiac hormone that is known to play an essential role in regulation of blood pressure and vascular endothelial function. However, the metabolic regulation of ANP is not fully understood. In patients suffering from metabolic syndrome hypertension, hyperinsulinemia and postprandial hyperglucagonemia are common features. It was the aim of the present study to evaluate the impact of glucagon on proANP and the possible mechanisms ...

ea0022p687 | Obesity | ECE2010

Glucagon stimulates cholecystokinin secretion in humans

Arafat Ayman , Otto Barbel , Adamidou Aikaterini , Spranger Joachim , Weickert Martin , Mohlig Matthias , Pfeiffer Andreas

Objectives: Gastrointestinal hormones are known to modulate satiety. The mechanisms underlying the impact of glucagon on satiety are so far unknown. However, it can be postulated that the glucagon-induced reduction in hunger feeling is mediated through changes in Cholecystokinin (CCK), which is known to play a role in meal termination. It was the aim of the present study to evaluate the impact of glucagon on CCK and the modulation of these effects in obesity.<p class="abst...

ea0020p464 | Obesity and Metabolism | ECE2009

Glucose-dependent insulinotropic polypeptide increases glucose-uptake in muscle- and adipocyte cells in vitro

Andres Janin , Biedasek Katrin , Goegebakan Ozlem , Mai Knut , Pfeiffer Andreas , Spranger Joachim

Glucose-dependent insulinotropic polypeptide (GIP) is an incretin hormone secreted by K-cells of proximal small intestine that is secreted postprandial in response to glucose and fat ingestion. Its effect is mediated through a GIP receptor (GIPR) which is widely expressed in different tissues, e.g. pancreatic islets, adipose tissue, skeletal muscle, adrenal cortex, heart, pituitary and in some regions of the brain. However, only the effect of GIP in pancreatic beta-cells is we...

ea0016p215 | Diabetes and cardiovascular diseases | ECE2008

Metabolic changes induced by glucose infusion in persons with Glu23Lys polymorphism

Bobbert Thomas , Mittag Marc , Mai Knut , Fischer Antje , Arafat Ayman , Pfeiffer AFH , Spranger Joachim

The development of diabetes mellitus type 2 (T2DM) is affected by genetic and lifestyle factors. Polymorphisms within the KIR6.2 gene seem to be associated with a higher risk for T2DM. The protein encoded by this gene represents a potassium channel, which plays an essential role in insulin and glucagon secretion by the pancreas.A hyperglycaemic clamp without insulin was performed over 2 h in 10 healthy persons with the Kir6.2 E23K wild type Glu/Glu, 10 a...

ea0016p217 | Diabetes and cardiovascular diseases | ECE2008

GC-tofMS metabolite profiling in the pancreatic beta cell line INS-1 under chronic glucose stress conditions

Gohring Isabel , Catchpole Gareth , Eckardt Anne , Willmitzer Lothar , Pfeiffer Andreas FH , Spranger Joachim

Background: Long term exposure of INS-1 beta cells to elevated glucose concentrations has been observed to impair insulin expression, a phenomenon termed as glucose toxicity. Despite its potential relevance in the progression of diabetes, the underlying mechanisms is not yet fully understood.Aim: Target analysis of single, or a sub-group of metabolites has until now been the method of choice in mammalian cells to investigate the pathological state in ter...

ea0081oc10.2 | Oral Communications 10: Diabetes, Obesity, Metabolism and Nutrition 3 | ECE2022

Success in implementing changes in macronutrients intake in a high-protein and high-unsaturated fatty acids dietary intervention: 36-months results of the NutriAct randomized controlled multi-center trial

Pletsch-Borba Laura , Wernicke Charlotte , Meyer Nina , Nguyen Thu , Pohrt Anne , Gerbracht Christiana , Pfeiffer Andreas , Spranger Joachim , Mai Knut

Background & Aims: NutriAct is a 36-month randomized controlled multi-center trial aiming to analyze the effects of a dietary pattern focusing on a high-protein and high-unsaturated fatty acids (UFA) intake on healthy aging. We aimed to explore changes in intake of macronutrients and determine factors associated with a successful modulation of dietary pattern after 36 months in elderly community dwelling participants.Methods: 502 participants were ra...

ea0016p201 | Diabetes and cardiovascular diseases | ECE2008

No genetic impact of the SOCS3-gene on T2DM

Fischer Antje , Fisher Eva , Moehlig Matthias , Osterhoff Martin , Weickert Martin O , Pfeifer Andreas FH , Boeing Heiner , Spranger Joachim

The diabetic epidemic is increasing worldwide. The suppressor of cytokine signaling 3 (SOCS3) provides a link between cytokine action and their negative consequences on insulin signaling. This potential influence on the development of T2DM makes the gene a potential candidate gene for type 2 diabetes.We investigated the one haplotype tagging single nucleotide polymorphism (htSNP) rs4969168 to examine the impact on T2DM in two independent study population...

ea0016p540 | Obesity | ECE2008

Retinol-binding protein 4 is independently associated with insulin resistance in PCOS women

Mohlig Matthias , Weickert Martin O , Ghadamgahi Elham , Arafat Ayman M , Spranger Joachim , Pfeiffer Andreas FH , Schofl Christof

Objective: Adiposity, insulin resistance, and hyperandrogenism are features of the polycystic ovary syndrome (PCOS). Retinol-binding protein 4 (RBP4) secreted from adipose and liver tissue has been linked to insulin resistance. We here address the impact of RBP4 on insulin resistance in PCOS and its usability to identify women with metabolic syndrome or impaired glucose metabolism (IGT or diabetes).Design: RBP4 was determined in plasma of 115 PCOS...

ea0014p19 | (1) | ECE2007

Insulin decreases IGF-I bioactivity in patients with impaired glucose tolerance and in healthy subjects

Arafat Ayman M , Frystyk Jan , Weickert Martin O , Spranger Joachim , Schöfl Christof , Möhlig Mathias

Objectives: Insulin resistance (IR) is a very common metabolic abnormality in obesity, which is often associated with reduced growth hormone (GH) secretion. GH deficiency is associated with increased in intra-abdominal fat and several parameters of the metabolic syndrome. IGF-I improves IR but the IGF-binding proteins are supposed to regulate its bioactivity although only little information exists. We postulated that the elevated insulin levels due to IR do not only suppress G...