Searchable abstracts of presentations at key conferences in endocrinology

ea0086ec1.1 | Early Career Prize Lecture Basic Science | SFEBES2022

The diverse and distinct roles of adipose tissue on metabolic heath

Suchacki Karla

Obesity and its associated cardiometabolic complications place a huge burden on global health. The key feature of obesity is increased white adipose tissue (WAT) mass, however the role of ‘lesser known’ depots such as bone marrow adipose tissue (BMAT) and brown adipose tissue (BAT) is not clear in adult humans. Over the last decade I have undertaken a variety of metabolic studies in both mice and humans to determine the metabolic role and regulation of BMAT, BAT and ...

ea0056oc3.3 | New insights in bone disorders | ECE2018

[18F]-FDG PET/CT imaging: A tool to reveal the metabolic functions of bone marrow adipose tissue

Suchacki Karla , Tavares Adriana , Sinton Matthew , Alcaide Carlos , Morton Nicholas , Cawthorn William

Introduction: White adipose tissue (WAT) and brown adipose tissue (BAT) are key regulators of systemic metabolic function. Bone marrow adipose tissue (BMAT) accounts for 10% of total adipose mass in healthy humans and therefore can be considered a third major adipose subtype. However, it is unclear if BMAT contributes to systemic energy homeostasis.Objectives: Assess [18F]-Fluorodeoxyglucose (FDG) uptake into bone and the marrow cavity (MC) fo...

ea0056gp58 | Bone and Osteoporosis | ECE2018

Investigating glucocorticoids as mediators of increased bone marrow adiposity during caloric restriction

Lovdel Andrea , Suchacki Karla , Sulston Richard J , Wallace Robert J , Macpherson Gavin , Stimson Roland H , Homer Natalie ZM , Chapman Karen E , Cawthorn William P

Background: Bone marrow adipose tissue (BMAT) comprises >10% of total adipose mass in healthy humans and further increases in diverse clinical conditions, including obesity/diabetes, osteoporosis and following caloric restriction (CR). However, why BMAT increases during CR remains unknown. One possibility is that this is mediated by glucocorticoid (GC) excess. GC action on target tissues depends on circulating and intracellular concentrations of t...

ea0094oc6.4 | Metabolism, Obesity and Diabetes | SFEBES2023

Human brown adipose tissue activity is regulated by the parasympathetic nervous system

Choong Kwok T'ng , Ramage Lynne E. , Gray Calum , Wakelin Sonia J. , JR van Beek Edwin , Kelman Alexandra , Semple Robert J. , Suchacki Karla J. , Stimson Roland H

Brown adipose tissue (BAT) is a therapeutic target for obesity and associated metabolic diseases, due to its role in non-shivering thermogenesis. BAT activation is mediated through sympathetic stimulation, but parasympathetic regulation of human BAT has not been demonstrated previously. We undertook RNA sequencing of human white and brown primary adipocytes to identify novel pathways regulating BAT. CHRM2 (encoding the muscarinic acetylcholine receptor 2) was the most differen...

ea0077oc3.1 | Metabolism, Obesity and Diabetes | SFEBES2021

The serotonin transporter SLC6A4 protects human brown adipose tissue from serotonin-mediated suppression of thermogenesis.

Choong Kwok T’ng , Suchacki Karla , Ramage Lynne , Kelman Alexandra , McNeill Ben , Rodney Stewart , Keegan Matthew , Gray Calum , Manning Jonathan , MacNaught Gillian , Fletcher Alison , Simpson Joanna , Carter Roderick , Morton Nicholas , Homer Natalie , van Beek Edwin , Wakelin Sonia , Stimson Roland

The recent discovery of brown adipose tissue (BAT) in adult humans, which generates heat to maintain body temperature in a cold environment, offers an exciting new strategy to treat obesity and metabolic disease, but our knowledge of human BAT activation is limited. To identify novel pathways regulating human BAT, we undertook RNA sequencing of human brown and white adipocytes. The gene SLC6A4 (encoding the serotonin transporter SERT) was one of the most highly differ...

ea0086oc5.1 | Metabolism, Obesity and Diabetes | SFEBES2022

Human brown adipose tissue demonstrates substantial choline uptake for incorporation into phosphatidylcholines

Suchacki Karla , Ramage Lynne , Gray Calum , Rodrguez Blanco Giovanny , Choong Kwok T'ng , Boyle Luke , MacNaught Gillian , Gregoriades Maria-lena , Wakelin Sonia , von Kriegsheim Alex , Finch Andrew , Patel Dilip , van Beek Edwin , Stimson Roland

Background: 18F-fluorodeoxyglucose (18FDG) PET is commonly used to quantify brown adipose tissue (BAT) mass/activity in humans but requires cold exposure. Rodent brown (BAds) but not white adipocytes (WAds) exhibit high choline content, thus we hypothesised that human BAT would demonstrate substantial 18F-fluorocholine (18FCH) uptake in vivo during warm and cold conditions.Methods: (1)...