Searchable abstracts of presentations at key conferences in endocrinology

ea0022s16.1 | Hot topics in pituitary disease | ECE2010

Pros and cons of large scale screening of Cushing's syndrome

Tabarin A , Perez P

Data from the literature suggest that endogenous Cushing’s syndrome (CS) is an unusual disorder in the general population with a reported incidence of 2–3 cases per 1 million inhabitants per year. Patients who are not cured from CS have a poor prognosis with an increased mortality due to cardiovascular disease and significant morbidity that impairs quality of life. Recently published screening studies performed in obese patients with type 2 diabetes or patients with ...

ea0037gp.09.08 | Nuclear receptors and signalling | ECE2015

Study of a possible interaction between reactive oxygen species and the mTORC1 pathway in the regulation of energy balance

Haissaguerre Magalie , Clark Samantha , Tabarin Antoine , Cota Daniela

Obesity and its consequent metabolic disorders are severe health problems. The mechanistic target of rapamycin complex 1 (mTORC1) pathway is an important hypothalamic integrator of the actions of nutrients and hormones on food intake (FI). Nutrient availability also affects the formation of reactive oxygen species (ROS) in the hypothalamus and regulates neuronal activity. In the present study, we hypothesise that modulation of mTORC1 activity mediates ROS effects on FI. To thi...

ea0070aep589 | Pituitary and Neuroendocrinology | ECE2020

The cushing’s collaborative patient survey results

Valassi Elena , Tabarin Antoine , Chiodini Iacopo , Feelders Richard , Andela Cornelie

Background: Early diagnosis of Cushing’s syndrome and initiation of effective treatment are essential to limit long-term morbidity and early mortality. However, diagnosis is often delayed due to the non-specificity of symptoms, or because symptoms are not recognised by physicians, leading to more complex treatment needs and a worsening of patient quality of life. A survey was conducted to better understand the patient experience of Cushing’s syndrome and the true b...

ea0063gp158 | Cushing's | ECE2019

Cortisol and cortisone assays in hair by mass spectrometry for the diagnosis of Cushing’s syndrome

Brossaud Julie , De Angeli Delia , Corcuff Jean-Benoit , Charret Lea , Tabarin Antoine

Background: According to the Endocrine Society’s guidelines, the screening and diagnosis of Cushing’s syndrome (CS) are based on the results of the overnight dexamethasone suppression test (DST), midnight salivary cortisol and 24-hour urinary free cortisol (UFC) measurements. These 3 tests reflect cortisol levels at the time of sampling without providing retrospective information. Hair cortisol concentration is a non-invasive way to measure cortisol exposure over lon...

ea0032p36 | Adrenal cortex | ECE2013

More than two successive measurements of late-night salivary cortisol are needed to accurately diagnose early-stage post-surgical recurrence of Cushing’s disease

Danet Marie , Asselineau Julien , Perez Paul , Vivot Alexandre , Laure Nunes Marie , Corcuff Jean-Benoit , Tabarin Antoine

The performance of late-night salivary cortisol (LNSC) and optimal number of samples to accurately diagnose post-operative recurrence of Cushing’s disease (CD) at an early-stage are unknown. We performed a retrospective analysis in a single tertiary-care center to compare the accuracy of multiple salivary sampling strategies to diagnose early-stage recurrences of CD. 36 patients in remission of CD after surgery were followed up for 69.2±10.6 mo with multiple successi...

ea0029p55 | Adrenal cortex | ICEECE2012

Urinary cortisol metabolites in corticotroph and adrenal tumours

Brossaud J. , Ducint D. , Gatta B. , Molimard M. , Tabarin A. , Corcuff J.

We investigated whether urinary cortisol metabolites could help to diagnose patients with cortisol oversecretion either with benign adrenal or corticotroph tumours.Liquid chromatography tandem mass spectrometric assay (LC-MSMS) of cortisol (F)m, cortisone, tetra-hydrocortisol (THF), allo-THF, tetra-hydrocortisone (THE), α-cortol, β-cortol, α-cortolone, α-cortolone and internal standard 6-α-methylprednisolone were assayed after de...

ea0026p334 | Obesity | ECE2011

Synergistic post-prandial deregulation of orexigenic and anorexigenic systems in obesity

Gatta-Cherifi B , Mattias I , Vallee M , Tabarin A , Marsicano G , Piazza P V , Cota D

Introduction: Obese humans have increased plasma levels of endocannabinoids like anandamide (AEA) and 2-arachydonoyl-glycerol (2-AG). However, it is unknown whether these orexigenic signals have a role in human eating behavior.Design: We measured plasma AEA and 2-AG levels in 12 normal weight (10 F, age: 39.1±3.7 years, body mass index (BMI): 21.0±0.6 kg/m2, waist circumference (WC): 73.8±2.9 cm) and 12 insulin-resistant obese s...

ea0022p36 | Adrenal | ECE2010

Free cortisol in plasma and saliva samples after synacthen testing in a routine exploration of patients in endocrinology

Brossaud Julie , Dolomie Laurence , Gatta Blandine , Tabarin Antoine , Corcuff Jean-Benoit

Cortisol ([C]) biological activity depends on its free fraction diffusion into the intracellular compartment. The diagnosis of adrenal insufficiency requires investigations such as the synacthen test and studies in intensive care units suggest that free plasma [C] could provide a better indicator of efficient [C] than total plasma [C]. We undertook this study to compare various determinations of free [C] levels with regard to adrenal status evaluated on total plasma [C] after ...

ea0014oc10.5 | Obesity & metabolism | ECE2007

Mice lacking CRF receptor type 1 (CRFR1) have reduced vulnerability to diet-induced obesity

Diz-Chaves Yolanda , Monsaingeon Maud , Penicaud Luc , Ricquier Daniel , Contarino Angelo , Tabarin Antoine

Evidence has accumulated about the involvement of the CRF system in the regulation of energy balance. The effects of CRF are mediated by two receptors: CRFR1 and CRFR2. The role of the CRFR1 in the regulation of energy balance is not well defined. To address this issue, adult male CRFR1 KO mice and WT littermates were given low fat (LFD) or high-fat (HFD) diets for 4 months. Under LFD no differences between genotypes were seen on body weight (BW) and caloric intake. KO mice ha...

ea0056p870 | Pituitary - Clinical | ECE2018

Safety and efficacy of long-acting Pasireotide monotherapy in acromegalic patients treated with a combination of first-generation somatostatin analogs and cabergoline or pegvisomant

Lasolle Helene , Ferriere Amandine , Nunes Marie-Laure , Haissaguerre Magalie , Tabarin Antoine

Introduction: Combination therapy using pegvisomant and cabergoline with first-generation long-acting somatostatin analogs (1GSSA) is a common procedure in acromegalic patients that are not fully controlled by surgery and 1GSSA. Pasireotide-LAR is a new multireceptor-targeted somatostatin receptor ligand that has superior efficacy over octreotide LAR to control GH and IGF1 levels. Little data is available about the efficacy and safety of pasireotide monotherapy in patients tre...