Searchable abstracts of presentations at key conferences in endocrinology

ea0086p155 | Adrenal and Cardiovascular | SFEBES2022

Glucocorticoid Excess Disrupts the NAD+ Metabolome Within Skeletal Muscle in Male and Female C57BL/6J Mice

Heising Silke , Heaselgrave Samuel , Morgan Stuart , Kabli Ali , Doig Craig , Tsintzas Kostas , Lavery Gareth

Introduction: Glucocorticoid excess (GE) causes severe metabolic dysfunction within skeletal muscle (SM) which includes reduced muscle accrual and increased proteolysis. The NAD+ metabolome is crucial for SM health and metabolic function, however, whether this is disrupted by GE remains unknown.Methods: Male and female C57BL/6J mice (n=12) were treated with a vehicle control or corticosterone (100 mg/l) ad libitum via drinking water for 3 weeks ...

ea0086oc4.6 | Adrenal and Cardiovascular | SFEBES2022

Glucocorticoid excess elevates metabolic rate via a 11β-HSD1 dependent mechanism in C57BL/6J mice

Heaselgrave Samuel , Heising Silke , Morgan Stuart , Kabli Ali , Sagmeister Michael , Hardy Rowan , Doig Craig , Morton Nicholas , Tsintzas Kostas , Lavery Gareth

Introduction: Glucocorticoids are vital metabolic regulators. However, glucocorticoid excess (GE) causes severe metabolic dysfunction, ultimately leading to Cushing’s Syndrome. This dysfunction is often dependent on the presence of the enzyme 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1). Whether GE also alters metabolic rate, and whether this is also dependent on 11β-HSD1, remains unclear.Methods: Male and female wild-type (WT) ...